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![Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202410334024.jpg "Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Flow cytometric analysis of MCF7 cells labeling AKT1/2/3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32017, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202416453243.jpg)
![Western Blot: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Western blot analysis of AKT1/2/3 on different lysates with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: MCF7 cell lysateLane 3: A549 cell lysateLane 4: U-2 OS cell lysateLane 5: NIH/3T3 cell lysateLysates/proteins at 15 µg/Lane.Predicted band size: 56 kDaObserved band size: 56 kDaExposure time: 30 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-3920248453538.jpg)
![Western Blot: p38 alpha [p Tyr323] Antibody [NBP3-23249] - A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38 alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38 alpha (Tyr-323) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23249_rabbit-p38-alpha-p-tyr323-pab-19920231410135.jpg "Western Blot: p38 alpha [p Tyr323] Antibody [NBP3-23249] - A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38 alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38 alpha (Tyr-323) (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: p38 alpha [p Tyr323] Antibody [NBP3-23249] - Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23249_rabbit-p38-alpha-p-tyr323-pab-19920231911472.jpg)
![Western Blot: SSH1 [p Ser978] Antibody [NBP3-23411] - Western blot of human recombinant SSH1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1 (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1 (Ser-978) (lanes 3, 4, 7, & 8).](http://images.novusbio.com/fullsize/nbp3-23411_rabbit-ssh1-p-ser978-pab-19920231820293.jpg "Western Blot: SSH1 [p Ser978] Antibody [NBP3-23411] - Western blot of human recombinant SSH1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1 (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1 (Ser-978) (lanes 3, 4, 7, & 8).")
![Immunocytochemistry/Immunofluorescence: SSH1 [p Ser978] Antibody [NBP3-23411] - Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-SSH1 (C-term.) and anti-SSH1 (Ser-978) antibodies, then detected using appropriate secondary antibody conjugated to Cy3 (Right panel). Phase image of corresponding PC12 cells (Left panel).](http://images.novusbio.com/fullsize/nbp3-23411_rabbit-ssh1-p-ser978-pab-19920231240373.jpg)
![Immunocytochemistry/Immunofluorescence: CREB [p Ser271] Antibody [NBP3-23250] - Immunocytochemical labeling of phosphorylated CREB in control and calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with rabbit polyclonal CREB (Ser-271). The antibody was detected using goat anti-rabbit DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23250_rabbit-creb-p-ser271-pab-1992023199286.jpg "Immunocytochemistry/Immunofluorescence: CREB [p Ser271] Antibody [NBP3-23250] - Immunocytochemical labeling of phosphorylated CREB in control and calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with rabbit polyclonal CREB (Ser-271). The antibody was detected using goat anti-rabbit DyLight(R) 594.")
![Western Blot: CREB [p Ser271] Antibody [NBP3-23250] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23250_rabbit-creb-p-ser271-pab-189202317355213.jpg)
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody [NBP3-23245] - Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and immunofluorescent images (right).](http://images.novusbio.com/fullsize/nbp3-23245_rabbit-caveolin-1-pab-199202317594410.jpg "Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody [NBP3-23245] - Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and immunofluorescent images (right).")
![Western Blot: Caveolin-1 Antibody [NBP3-23245] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23245_rabbit-caveolin-1-pab-19920231410121.jpg)
![Western Blot: SHC1 Antibody [NBP3-23392] - Western blot analysis of SHC1 expression in A431 cell lysate (lanes 1, 2, 3, & 4). The blots were probed with rabbit polyclonal anti-SHC1 (NBP3-23392) at 1:1000 (lane 1) or 1:4000 (lane 2) and mouse monoclonal SHC1 (C-terminal region) at 1:1000 (lane 3) or 1:4000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23392_rabbit-shc1-pab-19920231410125.jpg "Western Blot: SHC1 Antibody [NBP3-23392] - Western blot analysis of SHC1 expression in A431 cell lysate (lanes 1, 2, 3, & 4). The blots were probed with rabbit polyclonal anti-SHC1 (NBP3-23392) at 1:1000 (lane 1) or 1:4000 (lane 2) and mouse monoclonal SHC1 (C-terminal region) at 1:1000 (lane 3) or 1:4000 (lane 4).")
![Immunocytochemistry/Immunofluorescence: SHC1 Antibody [NBP3-23392] - Immunocytochemical labeling of SHC1 in paraformaldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal (top) and rabbit polyclonal (bottom NBP3-23392) SHC1 antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23392_rabbit-shc1-pab-18920231735525.jpg)
![Western Blot: Caveolin-1 Antibody (M281) [NBP3-23193] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23193_mouse-caveolin-1-mab-m281-119202316331613.jpg "Western Blot: Caveolin-1 Antibody (M281) [NBP3-23193] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (M281) [NBP3-23193] - Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and immunofluorescent images (right).](http://images.novusbio.com/fullsize/nbp3-23193_mouse-caveolin-1-mab-m281-19920231856940.jpg)
![Western Blot: CREB Antibody [NBP3-23243] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23243_rabbit-creb-pab-19920231856929.jpg "Western Blot: CREB Antibody [NBP3-23243] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP3-23243] - Immunocytochemical labeling of CREB in A431 that were fixed in paraformaldehyde and permeabilized using NP-40. The cells were labeled with rabbit polyclonal CREB (N-terminal region). The antibody was detected using goat anti-rabbit DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23243_rabbit-creb-pab-199202312523630.jpg)
![Immunocytochemistry/Immunofluorescence: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Immunocytochemical labeling of phosphorylated CREB in calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with mouse monoclonal CREB (Ser-133). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23244_mouse-creb-p-ser133-mab-m415-19920231915415.jpg "Immunocytochemistry/Immunofluorescence: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Immunocytochemical labeling of phosphorylated CREB in calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with mouse monoclonal CREB (Ser-133). The antibody was detected using goat anti-mouse DyLight(R) 594.")
![Western Blot: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23244_mouse-creb-p-ser133-mab-m415-199202312523628.jpg)
![Western Blot: IRS1 [p Ser307] Antibody [NBP1-78078] - Band at ~180 kDa believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate. The pronounced staining of the band at 180 kDa is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction. The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).](http://images.novusbio.com/fullsize/IRS1-[p-Ser307]-Antibody-Western-Blot-NBP1-78078-img0001.jpg "Western Blot: IRS1 [p Ser307] Antibody [NBP1-78078] - Band at ~180 kDa believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate. The pronounced staining of the band at 180 kDa is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction. The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).")
![Western Blot: Histone H3.1 [ac Lys14] Antibody (3H3) [NBP3-26533] - Positive Western Blot detected in Hela whole cell lysate. HepG2 whole cell lysate. SH-SY5Y whole cell lysate. Rat brain tissue. All lanes: Histone H3.1 [ac Lys14] Antibody at 0.75 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa](http://images.novusbio.com/fullsize/nbp3-26533_rabbit-histone-h3-1-ac-lys-14-mab-3h3-28220241152513.jpg "Western Blot: Histone H3.1 [ac Lys14] Antibody (3H3) [NBP3-26533] - Positive Western Blot detected in Hela whole cell lysate. HepG2 whole cell lysate. SH-SY5Y whole cell lysate. Rat brain tissue. All lanes: Histone H3.1 [ac Lys14] Antibody at 0.75 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa")
![Immunocytochemistry/Immunofluorescence: Histone H3.1 [ac Lys14] Antibody (3H3) [NBP3-26533] - Staining of Hela cells(treated by 15mM sodium butyrate for 30min) with Histone H3.1 [ac Lys14] Antibody (3H3) at 1:46, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26533_rabbit-histone-h3-1-ac-lys-14-mab-3h3-28220241249361.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.1 [ac Lys14] Antibody (3H3) [NBP3-26533] - Analysis of Histone H3.1 [ac Lys14] Antibody (3H3) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26533_rabbit-histone-h3-1-ac-lys-14-mab-3h3-282202412493623.jpg)
![Immunocytochemistry/Immunofluorescence: SHP-2/PTPN11 Antibody [NBP2-42920] - Analysis of Denque virus 2-infected BHK-21, using NS1 (Dengue virus) antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42920-img0001.jpg "Immunocytochemistry/Immunofluorescence: SHP-2/PTPN11 Antibody [NBP2-42920] - Analysis of Denque virus 2-infected BHK-21, using NS1 (Dengue virus) antibody at 1:2000 dilution.")
