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![Immunoprecipitation: eIF2B epsilon Antibody [NBP3-35116] - Immunoprecipitation analysis of 200 ug extracts of A-549 cells using 3 ug eIF2B epsilon(eIF2B epsilon(EIF2B5)) antibody ( A10263). Western blot was performed from the immunoprecipitate using eIF2B epsilon(eIF2B epsilon(EIF2B5)) antibody ( A10263) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35116_rabbit-eif2b-epsilon-pab-912202414154314.jpg "Immunoprecipitation: eIF2B epsilon Antibody [NBP3-35116] - Immunoprecipitation analysis of 200 ug extracts of A-549 cells using 3 ug eIF2B epsilon(eIF2B epsilon(EIF2B5)) antibody ( A10263). Western blot was performed from the immunoprecipitate using eIF2B epsilon(eIF2B epsilon(EIF2B5)) antibody ( A10263) at a dilution of 1:1000.")
![Immunocytochemistry/ Immunofluorescence: eIF2B epsilon Antibody [NBP3-35116] - Immunofluorescence analysis of L929 cells using eIF2B epsilon(eIF2B epsilon(EIF2B5)) Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35116_rabbit-eif2b-epsilon-pab-912202415161839.jpg)
![Western Blot: eIF2B epsilon Antibody [NBP3-35116] - Western blot analysis of various lysates using eIF2B epsilon(EIF2B5) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35116_rabbit-eif2b-epsilon-pab-91220241519514.jpg)
![Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.](http://images.novusbio.com/fullsize/PLK1-[p-Ser482--p-Ser486--p-Ser490]-Antibody-Western-Blot-NB300-278-img0001.jpg "Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.")
![Western Blot: Dynamin [p Ser778] Antibody [NB300-210] - Analysis of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment.](http://images.novusbio.com/fullsize/Dynamin-[p-Ser778]-Antibody-Western-Blot-NB300-210-img0001.jpg "Western Blot: Dynamin [p Ser778] Antibody [NB300-210] - Analysis of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment.")
![Western Blot: NMDAR2B [p Tyr1336] Antibody [NB300-295] - Analysis of Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is shown in the second lane (lambda-phosphatase: lambda Ptase). The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr1336 NMDA NR2B subunit. The immunolabeling of NR2B is completely eliminated by treatment with lambda Ptase.](http://images.novusbio.com/fullsize/NMDAR2B-[p-Tyr1336]-Antibody-Western-Blot-NB300-295-img0004.jpg "Western Blot: NMDAR2B [p Tyr1336] Antibody [NB300-295] - Analysis of Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is shown in the second lane (lambda-phosphatase: lambda Ptase). The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr1336 NMDA NR2B subunit. The immunolabeling of NR2B is completely eliminated by treatment with lambda Ptase.")
![Immunohistochemistry: NMDAR2B [p Tyr1336] Antibody [NB300-295] - Staining of Mouse dentate gyrus 48 hr post TMT treatment showing NR2B when phosphorylated at Tyr1336 in green and nuclei in blue.](http://images.novusbio.com/fullsize/NMDAR2B-[p-Tyr1336]-Antibody-Immunohistochemistry-NB300-295-img0002.jpg)
![Western Blot: GluR1 [p Ser845] Antibody [NB300-171] - Rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/GluR1-[p-Ser845]-Antibody-Western-Blot-NB300-171-img0003.jpg "Western Blot: GluR1 [p Ser845] Antibody [NB300-171] - Rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salf diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-2G4-Western-Blot-NBP2-59382-img0003.jpg "Western Blot: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salf diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.")
![Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Immunohistochemistry analysis using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4 (NBP2-59382). Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody (NBP2-59382) at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-2G4-Immunohistochemistry-NBP2-59382-img0005.jpg)
![Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Immunohistochemistry analysis using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4 (NBP2-59382). Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody (NBP2-59382) at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-2G4-Immunohistochemistry-NBP2-59382-img0006.jpg)
![Western Blot: MARCKS [p Ser152, p Ser156] Antibody [NB500-140] - Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Ser152,156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser 152,156 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/MARCKS-[p-Ser152-p-Ser156]-Antibody-Western-Blot-NB500-140-img0002.jpg "Western Blot: MARCKS [p Ser152, p Ser156] Antibody [NB500-140] - Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Ser152,156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser 152,156 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: MEK5 Antibody [NBP1-97478] - Analysis of MEK5, pAb: Lane 1: Jurkat, Lane 2: Mouse Testes, Lane 3: Rat Brain](http://images.novusbio.com/fullsize/MEK5-Antibody-Western-Blot-NBP1-97478-img0001.jpg "Western Blot: MEK5 Antibody [NBP1-97478] - Analysis of MEK5, pAb: Lane 1: Jurkat, Lane 2: Mouse Testes, Lane 3: Rat Brain")
![Western Blot: Rad17 [p Ser656] Antibody (JE63-30) [NBP3-32792] - Western blot analysis of Rad17 on different lysates with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 3: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, then the membrane treated with pp for 1 hour Lane 4: NIH/3T3 cell lysate Lane 5: NIH/3T3 treated with 50ng/mL Calyculin A for 45 minutes cell lysate Lane 6: NIH/3T3 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 77 kDa Observed band size: 77 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32792) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32792_rabbit-rad17-p-ser656-mab-je63-30-288202412442977.jpg "Western Blot: Rad17 [p Ser656] Antibody (JE63-30) [NBP3-32792] - Western blot analysis of Rad17 on different lysates with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 3: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, then the membrane treated with pp for 1 hour Lane 4: NIH/3T3 cell lysate Lane 5: NIH/3T3 treated with 50ng/mL Calyculin A for 45 minutes cell lysate Lane 6: NIH/3T3 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 77 kDa Observed band size: 77 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32792) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Rad17 [p Ser656] Antibody (JE63-30) [NBP3-32792] - Immunocytochemistry analysis of HeLa cells treated with or without 100ng/mL Calyculin A for 30 minutes labeling Rad17 with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32792_rabbit-rad17-p-ser656-mab-je63-30-2920241114833.jpg)
![Flow Cytometry: Rad17 [p Ser656] Antibody (JE63-30) [NBP3-32792] - Flow cytometric analysis of HeLa cells treated with or without 100ng/mL Calyculin A for 30 minutes labeling Rad17. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32792, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32792_rabbit-rad17-p-ser656-mab-je63-30-29202416453256.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Detection of phosphorylated CD3 zeta (EM-26; light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. Total CD3 zeta indicated in green, actin cytoskeleton in red.](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr72-Antibody-EM-26-Immunocytochemistry-Immunofluorescence-NBP2-62193-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Detection of phosphorylated CD3 zeta (EM-26; light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. Total CD3 zeta indicated in green, actin cytoskeleton in red.")
![Western Blot: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr72-Antibody-EM-26-Western-Blot-NBP2-62193-img0001.jpg)
![Western Blot: EZH2/KMT6 Antibody (PSH04-14) [NBP3-32509] - Western blot analysis of EZH2/KMT6 on different lysates with Rabbit anti-EZH2/KMT6 antibody (NBP3-32509) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: HeLa cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32509) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32509_rabbit-ezh2-kmt6-mab-psh04-14-278202415295564.jpg "Western Blot: EZH2/KMT6 Antibody (PSH04-14) [NBP3-32509] - Western blot analysis of EZH2/KMT6 on different lysates with Rabbit anti-EZH2/KMT6 antibody (NBP3-32509) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: HeLa cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32509) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: EZH2/KMT6 Antibody (PSH04-14) [NBP3-32509] - Immunocytochemistry analysis of HeLa cells labeling EZH2/KMT6 with Rabbit anti-EZH2/KMT6 antibody (NBP3-32509) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EZH2/KMT6 antibody (NBP3-32509) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32509_rabbit-ezh2-kmt6-mab-psh04-14-29202410234245.jpg)