| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35082-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05380-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38396-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35791-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-16890-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16137-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05409-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1156-25ul | |||
![Immunocytochemistry/ Immunofluorescence: ATG13 Antibody [NBP3-35082] - Immunofluorescence analysis of NIH/3T3 cells using ATG13 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35082_rabbit-atg13-pab-912202414123814.jpg "Immunocytochemistry/ Immunofluorescence: ATG13 Antibody [NBP3-35082] - Immunofluorescence analysis of NIH/3T3 cells using ATG13 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: ATG13 Antibody [NBP3-35082] - Immunofluorescence analysis of HeLa cells using ATG13 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35082_rabbit-atg13-pab-912202415143670.jpg)
![Western Blot: ATG13 Antibody [NBP3-35082] - Western blot analysis of various lysates, using ATG13 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 25s.](http://images.novusbio.com/fullsize/nbp3-35082_rabbit-atg13-pab-91220241416308.jpg)
![Immunoprecipitation: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916). Western blot was performed from the immunoprecipitate using Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-p-Ser293-Antibody-9S3I4-Immunoprecipitation-NBP3-16916-img0002.jpg "Immunoprecipitation: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916). Western blot was performed from the immunoprecipitate using Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916) at a dilition of 1:1000.")
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] -analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb at1:23000 dilution.NIH/3T3 cells were treated by CIP (20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-western-blot-75202483541..jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] -analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb at 1:23000 dilution.293T cells were treated by lambda -PP mixed solution (1ul) at 30 c for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25uμg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-western-blot-75202483713..jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (6E3J0) [NBP3-16916] - Western blot analysis of lysates from HeLa cells, using Pyruvate Dehydrogenase E1-alpha subunit Rabbit mAb at 1:23000 dilution.293T cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-52202511383736.jpg)
![Western Blot: Elk-1 [p Ser383] Antibody [NBP3-05380] - Western blot analysis of extracts of Jurkat cells, using Elk-1 antibody (NBP3-05380) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Elk-1-[p-Ser383]-Antibody-Western-Blot-NBP3-05380-img0002.jpg "Western Blot: Elk-1 [p Ser383] Antibody [NBP3-05380] - Western blot analysis of extracts of Jurkat cells, using Elk-1 antibody (NBP3-05380) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-52202516283228.jpg)
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-5220251640288.jpg)
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Mouse cerebellum tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-52202516345237.jpg)
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-5220251636368.jpg)
![Western Blot: CDC16 Antibody [NBP3-38396] - Western blot analysis of various lysates using CDC16 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38396_rabbit-cdc16-pab-91220241517115.jpg "Western Blot: CDC16 Antibody [NBP3-38396] - Western blot analysis of various lysates using CDC16 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunohistochemistry: CDC16 Antibody [NBP3-38396] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CDC16 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38396_rabbit-cdc16-pab-9122024158123.jpg)
![Immunohistochemistry: CDC16 Antibody [NBP3-38396] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using CDC16 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38396_rabbit-cdc16-pab-912202414101810.jpg)
![Western Blot: NEK9 Antibody [NBP3-35791] - Western blot analysis of various lysates using NEK9 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-35791_rabbit-nek9-pab-912202414213730.jpg "Western Blot: NEK9 Antibody [NBP3-35791] - Western blot analysis of various lysates using NEK9 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.")
![Immunohistochemistry: NEK9 Antibody [NBP3-35791] - Immunohistochemistry analysis of paraffin-embedded Human colon using NEK9 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35791_rabbit-nek9-pab-912202415161831.jpg)
![Immunohistochemistry: NEK9 Antibody [NBP3-35791] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using NEK9 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35791_rabbit-nek9-pab-91220241418144.jpg)
![Western Blot: VASP [p Ser239] Antibody [NBP3-16890] - Western blot analysis of extracts of C6 cells, using VASP antibody (NBP3-16890) at 1:1000 dilution.C6 cells were treated by Forskolin (10 uM) at 37C for 1 hour after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/VASP-[p-Ser239]-Antibody-Western-Blot-NBP3-16890-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: VASP [p Ser239] Antibody [NBP3-16890] - Western blot analysis of extracts of C6 cells, using VASP antibody (NBP3-16890) at 1:1000 dilution.C6 cells were treated by Forskolin (10 uM) at 37C for 1 hour after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: VASP [p Ser239] Antibody - Azide and BSA Free [NBP3-16890] - Immunohistochemistry analysis of paraffin-embedded Human placenta using VASP Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16890_rabbit-vasp-p-ser-239-pab-52202511383778.jpg)
![Immunohistochemistry: VASP [p Ser239] Antibody - Azide and BSA Free [NBP3-16890] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using VASP Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16890_rabbit-vasp-p-ser-239-pab-522025125493.jpg)
![Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from NIH/3T3 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16137_rabbit-histone-h4-lactyl-lys-5-pab-52202511392278.jpg "Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from NIH/3T3 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from C6 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. C6 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16137_rabbit-histone-h4-lactyl-lys-5-pab-52202511374158.jpg)
![Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from MCF7 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. MCF7 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16137_rabbit-histone-h4-lactyl-lys-5-pab-52202511365166.jpg)
![Dot Blot: Histone H4 [Lactyl Lys5] Antibody [NBP3-16137] - Dot-blot analysis of all sorts of peptides using Histone H4 antibody (NBP3-16137) at 1:4000 dilution.](http://images.novusbio.com/fullsize/Histone-H4-[Lactyl-Lys5]-Antibody-Dot-Blot-NBP3-16137-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-05409] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CDK2 antibody (NBP3-05409).](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Immunohistochemistry-Paraffin-NBP3-05409-img0001.jpg "Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-05409] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CDK2 antibody (NBP3-05409).")
![Western Blot: CDK2 [p Thr160] Antibody [NBP3-05409] - analysis of C6, using Phospho-CDK2-T160 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05409_rabbit-polyclonal-cdk2-p-thr160-antibody-western-blot-159202383355..jpg)
![Western Blot: CDK2 [p Thr160] Antibody - Azide and BSA Free [CDK2] - Western blot analysis of lysates from C6 cells using CDK2 Rabbit pAb at 1:1000 dilution. C6 cells were treated by Nocodazole (10 ng/ml) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-05409_rabbit-polyclonal-cdk2-p-thr160-antibody-52202516345255.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NB100-665-img0001.jpg "Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]")
![Western Blot: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Western blot of mouse heart lysate showing specific immunolabeling of ~25k cTnI protein phosphorylated at Ser150 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-p-Ser150-Antibody-Western-Blot-NBP2-29526-img0002.jpg "Western Blot: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Western blot of mouse heart lysate showing specific immunolabeling of ~25k cTnI protein phosphorylated at Ser150 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.")
![Immunofluorescence: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Fixed adult mouse cardiomyocyte were stained with anti-Pim-1 and anti-cTnI antibodies and then processed for fluorescentstaining analysis. From Zhu et al.: Phosphorylation of cTnI by Pim1 Kinase, Cell Physiol Biochem 2018;45:2174-2186.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-p-Ser150-Antibody-Immunofluorescence-NBP2-29526-img0003.jpg)
![Immunoprecipitation: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Adult mouse heart tissue samples were extracted and immunoprecipitated with either anti-Pim-1 antibody or control IgG and then analyzed by Westernblot. From Zhu et al.: Phosphorylation of cTnI by Pim1 Kinase, Cell Physiol Biochem 2018;45:2174-2186.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-p-Ser150-Antibody-Immunoprecipitation-NBP2-29526-img0004.jpg)
![Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-278202415212460.jpg "Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Immunocytochemistry analysis of 293T cells labeling ERK3/MAPK6 with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-2920241192388.jpg)
![Flow Cytometry: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Flow cytometric analysis of 293T cells labeling ERK3/MAPK6. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32567, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-392024884681.jpg)