| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9355-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82009-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-19541-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91660-25ul | |||
![Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: NBP3-32725 IP in HeLa cell lysate<br/> Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 24 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2682024158269.jpg "Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: NBP3-32725 IP in HeLa cell lysate<br/> Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 24 seconds; ECL")
![Immunohistochemistry: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-PCNA antibody (NBP3-32725) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32725) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2982024149267.jpg)
![Immunocytochemistry/ Immunofluorescence: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunocytochemistry analysis of HeLa cells labeling PCNA with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-29202410484872.jpg)
![Western blot analysis on different lysates with NBP3-32725 at 1/20,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: HCT 116 cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: RAW264.7 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: L6 cell lysate (15 µg/Lane) Lane 8: Mouse spleen tissue lysate (15 µg/Lane) Lane 9: Rat spleen tissue lysate (15 µg/Lane) Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32725 at 1/20,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32725_-western-blot-639204590075059307.jpg)
![Western blot analysis on different lysates with NBP3-32725 at 1/20,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: Raji cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32725 at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32725_-western-blot-639204597792896872.jpg)
![Western Blot: PKC delta Antibody (M242) [NBP3-23307] - Western blot analysis of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-PKC delta (N-terminal region) at 1:125 (lane 1) and 1:500 (lane 2).](http://images.novusbio.com/fullsize/nbp3-23307_mouse-pkc-delta-mab-m242-18920231735527.jpg "Western Blot: PKC delta Antibody (M242) [NBP3-23307] - Western blot analysis of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-PKC delta (N-terminal region) at 1:125 (lane 1) and 1:500 (lane 2).")
![Immunocytochemistry/Immunofluorescence: PKC delta Antibody (M242) [NBP3-23307] - Immunocytochemical labeling of PKC delta in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal PKC delta (N-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23307_mouse-pkc-delta-mab-m242-1192023166534.jpg)
![Western Blot: PKC theta Antibody (M217) [NBP3-23308] - Western blot analysis of PKC theta in human Jurkat cell lysate. The blot was probed with anti-PKC theta at 1:250 (lane 1) and 1:1000 (lane 2).](http://images.novusbio.com/fullsize/nbp3-23308_mouse-pkc-theta-mab-m217-19920231992823.jpg "Western Blot: PKC theta Antibody (M217) [NBP3-23308] - Western blot analysis of PKC theta in human Jurkat cell lysate. The blot was probed with anti-PKC theta at 1:250 (lane 1) and 1:1000 (lane 2).")
![Immunocytochemistry/Immunofluorescence: PKC theta Antibody (M217) [NBP3-23308] - Immunocytochemical labeling of PKC theta in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal PKC theta (N-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23308_mouse-pkc-theta-mab-m217-19920231410124.jpg)
![Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Western-Blot-MAB9355-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions")
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - An intracellular stain was performed on MCF7 cells with HSP27 [p Ser78] Antibody MAB9355 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Flow-Cytometry-MAB9355-img0003.jpg)
![Simple Western: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Lane vies shows a specific band for HSP27 using 0.2 mg/mL MCF-7 -/+ UV cell lysates and antibody at 5 ug/mL. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 32 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Simple-Western-MAB9355-img0004.jpg)
![Western Blot: MCM2 [p Ser41] Antibody [NB100-2569] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Western-Blot-NB100-2569-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM2 [p Ser41] Antibody [NB100-2569] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM2 [p Ser41] Antibody [NB100-2569] - Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) used at a dilution of 1:200 (1 ug/ml).](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Immunohistochemistry-NB100-2569-img0032.jpg)
![Flow Cytometry: MCM2 [p Ser41] Antibody [NB100-2569] - Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10e6 cells were stained with 0.1 mcg/ml anti-phospho- MCM2 (pS40/41) in a 150 mcl volume. DNA content was simultaneously analyzed via PI stain](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Flow-Cytometry-NB100-2569-img0002.jpg)
![Western Blot: FOXO3 [p Ser253] Antibody [NB100-81929] - Analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (Lane 3 and 4).](http://images.novusbio.com/fullsize/FOXO3-[p-Ser253]-Antibody-Western-Blot-NB100-81929-img0007.jpg "Western Blot: FOXO3 [p Ser253] Antibody [NB100-81929] - Analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (Lane 3 and 4).")
![Immunohistochemistry-Paraffin: FOXO3 [p Ser253] Antibody [NB100-81929] - Analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody.](http://images.novusbio.com/fullsize/FOXO3-[p-Ser253]-Antibody-Immunohistochemistry-Paraffin-NB100-81929-img0006.jpg)
![Western Blot: FOXO3 [p Ser253] Antibody [NB100-81929] - Western Blot analysis of HeLa cells using NB100-81929 diluted at 1:2000](http://images.novusbio.com/fullsize/nb100-81929_rabbit-polyclonal-foxo3-p-ser253-antibody-23420241545354.jpg)
![Immunocytochemistry/Immunofluorescence: FOXO3 [p Ser253] Antibody [NB100-81929] - Staining of methanol-fixed HeLa cells using NB100-81929 (red).](http://images.novusbio.com/fullsize/nb100-81929_rabbit-polyclonal-foxo3-p-ser253-antibody-113202515145448.jpg)
![Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Western-Blot-NB100-82009-img0001.jpg "Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).")
![<b>Biological Strategies Validation. </b>Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), using SAPK/JNK (Phospho-Thr183) Antibody](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Western-Blot-NB100-82009-img0003.jpg)
![Immunohistochemistry: JNK1 [p Thr183] Antibody [NB100-82009] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82009](http://images.novusbio.com/fullsize/nb100-82009_rabbit-polyclonal-jnk1-p-thr183-antibody-103202523464547.jpg)
![Western Blot: MCAM/CD146 Antibody (S05-5A2) [NBP3-19541] - Western blot analysis of MCAM/CD146 expression in (1) A375 cell lysate; (2) HUVEC cell lysate](http://images.novusbio.com/fullsize/nbp3-19541_rabbit-mcam-cd146-mab-s05-5a2-234202415232512.jpg "Western Blot: MCAM/CD146 Antibody (S05-5A2) [NBP3-19541] - Western blot analysis of MCAM/CD146 expression in (1) A375 cell lysate; (2) HUVEC cell lysate")
![Immunohistochemistry-Paraffin: MCAM/NBP3-19541 (S05-5A2) [NBP3-19541] - Immunohistochemical analysis of paraffin-embedded human uterus, using NBP3-19541.](http://images.novusbio.com/fullsize/nbp3-19541_rabbit-mcam-cd146-mab-s05-5a2-2342024155516.jpg)
![Western Blot: MCAM/CD146 Antibody (S05-5A2) [NBP3-19541] - All lanes use NBP3-19541 at 1:5000 dilution for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-19541_rabbit-mcam-cd146-mab-s05-5a2-23420241446226.jpg)
![Immunocytochemistry/ Immunofluorescence: MCAM/CD146 Antibody (S05-5A2) [NBP3-19541] - Immunofluorescent analysis of A375 cells, using NBP3-19541.](http://images.novusbio.com/fullsize/nbp3-19541_rabbit-mcam-cd146-mab-s05-5a2-234202415453511.jpg)
![SDS-Page: Recombinant Human STAT3 Protein [H00006774-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006774-P01-1.jpg "SDS-Page: Recombinant Human STAT3 Protein [H00006774-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91660 (A) shows similar protein distribution across tissues to independent antibody NBP1-91659 (B).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91660 (A) shows similar protein distribution across tissues to independent antibody NBP1-91659 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91660-img0004.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human pancreas shows no postivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0015.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0010.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0013.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human liver shows moderate membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0014.jpg)
