| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-22436-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84532-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9355-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22376SS | |||
| Azide and BSA Free | NBP2-80812 | |||
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (1M7.8.1) [NBP2-22436] - Analysis of CD44 (green) showing staining in the membrane of BAF-3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CD44-Antibody-1M7-8-1-Immunocytochemistry-Immunofluorescence-NBP2-22436-img0004.jpg "Immunocytochemistry/Immunofluorescence: CD44 Antibody (1M7.8.1) [NBP2-22436] - Analysis of CD44 (green) showing staining in the membrane of BAF-3 cells (right) compared to a negative control without primary antibody (left).")
![Flow Cytometry: CD44 Antibody (1M7.8.1) [NBP2-22436] - Analysis of CD44 in Hela cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-1M7-8-1-Flow-Cytometry-NBP2-22436-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (1M7.8.1) [NBP2-22436] - Analysis of CD44 (green) showing staining in the membrane of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CD44-Antibody-1M7-8-1-Immunocytochemistry-Immunofluorescence-NBP2-22436-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (1M7.8.1) [NBP2-22436] - Analysis of CD44 (green) showing staining in the membrane and cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CD44-Antibody-1M7-8-1-Immunocytochemistry-Immunofluorescence-NBP2-22436-img0005.jpg)
![Flow Cytometry: CD44 Antibody (1M7.8.1) [NBP2-22436] - Analysis of CD44 in mouse splenocytes (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-1M7-8-1-Flow-Cytometry-NBP2-22436-img0001.jpg)
![Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Staining of liver lysates (A) Human with antibody at 0.1 ug/mL, Mouse (B) and Pig (C) with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Western-Blot-NB100-1448-img0007.jpg "Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Staining of liver lysates (A) Human with antibody at 0.1 ug/mL, Mouse (B) and Pig (C) with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1448-img0009.jpg)
![Flow Cytometry: 58K Golgi Protein Antibody [NB100-1448] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Flow-Cytometry-NB100-1448-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1448-img0008.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of Mouse (A) and Rat (B) Skin lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NB100-2465-img0007.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of Mouse (A) and Rat (B) Skin lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of paraffin embedded human skin. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Image shows strong nuclear staining in selected cells of the basal layer.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NB100-2465-img0004.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - (0.1ug/ml) staining of human skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NB100-2465-img0003.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of human skin lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NB100-2465-img0005.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of A431 (A) and U2OS (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NB100-2465-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC16 Antibody (7Y1Y10) [NBP3-33529] - Immunofluorescence analysis of U-2 OS cells using CDC16 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33529_rabbit-cdc16-mab-7y1y10-1012202411421094.jpg "Immunocytochemistry/ Immunofluorescence: CDC16 Antibody (7Y1Y10) [NBP3-33529] - Immunofluorescence analysis of U-2 OS cells using CDC16 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: CDC16 Antibody (7Y1Y10) [NBP3-33529] - Western blot analysis of lysates from NIH/3T3 cells, using CDC16 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33529_rabbit-cdc16-mab-7y1y10-1012202411364678.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC16 Antibody (7Y1Y10) [NBP3-33529] - Immunofluorescence analysis of C6 cells using CDC16 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33529_rabbit-cdc16-mab-7y1y10-101220241136082.jpg)
![Immunohistochemistry: CDC16 Antibody (7Y1Y10) [NBP3-33529] - Immunohistochemistry analysis of paraffin-embedded Human colon using CDC16 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33529_rabbit-cdc16-mab-7y1y10-1012202411392985.jpg)
![Immunohistochemistry: CDC16 Antibody (7Y1Y10) [NBP3-33529] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using CDC16 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33529_rabbit-cdc16-mab-7y1y10-101220241136072.jpg)
![Immunohistochemistry: CDC16 Antibody (7Y1Y10) [NBP3-33529] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using CDC16 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33529_rabbit-cdc16-mab-7y1y10-1012202411343387.jpg)
![Western Blot: VASP [p Ser157] Antibody [NBP3-05448] - Western blot analysis of extracts of A431 cells, using VASP antibody (NBP3-05448) at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/VASP-[p-Ser155]-Antibody-Western-Blot-NBP3-05448-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: VASP [p Ser157] Antibody [NBP3-05448] - Western blot analysis of extracts of A431 cells, using VASP antibody (NBP3-05448) at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded mouse lung using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/VASP-[p-Ser155]-Antibody-Immunohistochemistry-Paraffin-NBP3-05448-img0004.jpg)
![Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded rat brain using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/VASP-[p-Ser155]-Antibody-Immunohistochemistry-Paraffin-NBP3-05448-img0002.jpg)
![Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded human colon using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/VASP-[p-Ser155]-Antibody-Immunohistochemistry-Paraffin-NBP3-05448-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: VASP [p Ser157] Antibody [NBP3-05448] - Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug VASP pAb (NBP3-05448). Western blot was performed from the immunoprecipitate using VASP pAb (NBP3-05448) at a dilution of 1:1000. A-431 cells were treated by Forskolin (10 uM) at 37C for 30 minutes after serum-starvation overnight.](http://images.novusbio.com/fullsize/VASP-[p-Ser155]-Antibody-Immunoprecipitation-NBP3-05448-img0001.jpg)
![Western Blot: VASP [p Ser157] Antibody - Azide and BSA Free [VASP] - Western blot analysis of lysates from HEL cells using VASP Rabbit pAb at 1:1000 dilution. HEL cells were treated by Forskolin (20 uM) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.8s.](http://images.novusbio.com/fullsize/nbp3-05448_rabbit-polyclonal-vasp-p-ser155-antibody-5220251629390.jpg)
![Western Blot: Cdc2 [p Tyr15] Antibody [NB500-142] - Western Blot. Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation. The labeling by the antibody to phospho Tyr15 cdc2 is specifically blocked by the Ser15 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).](http://images.novusbio.com/fullsize/Cdc2-[p-Tyr15]-Antibody-Western-Blot-NB500-142-img0001.jpg "Western Blot: Cdc2 [p Tyr15] Antibody [NB500-142] - Western Blot. Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation. The labeling by the antibody to phospho Tyr15 cdc2 is specifically blocked by the Ser15 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).")
![Western Blot: MCM2 [p Ser41] Antibody [NB100-2569] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Western-Blot-NB100-2569-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM2 [p Ser41] Antibody [NB100-2569] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM2 [p Ser41] Antibody [NB100-2569] - Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) used at a dilution of 1:200 (1 ug/ml).](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Immunohistochemistry-NB100-2569-img0032.jpg)
![Flow Cytometry: MCM2 [p Ser41] Antibody [NB100-2569] - Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10e6 cells were stained with 0.1 mcg/ml anti-phospho- MCM2 (pS40/41) in a 150 mcl volume. DNA content was simultaneously analyzed via PI stain](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Flow-Cytometry-NB100-2569-img0002.jpg)
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human skeletal muscle shows low positivity in myocytes as expected.](http://images.novusbio.com/fullsize/M-CSFR-CD115-Antibody-Immunohistochemistry-Paraffin-NBP1-84532-img0016.jpg "Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human skeletal muscle shows low positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/M-CSFR-CD115-Antibody-Immunohistochemistry-Paraffin-NBP1-84532-img0012.jpg)
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/M-CSFR-CD115-Antibody-Immunohistochemistry-Paraffin-NBP1-84532-img0014.jpg)
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human rectum shows weak to moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/M-CSFR-CD115-Antibody-Immunohistochemistry-Paraffin-NBP1-84532-img0015.jpg)
![Western Blot: PLA2G4A Antibody [NBP1-31344] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLA2G4A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PLA2G4A-Antibody-Western-Blot-NBP1-31344-img0014.jpg "Western Blot: PLA2G4A Antibody [NBP1-31344] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLA2G4A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PLA2G4A Antibody [NBP1-31344] - Analysis of HeLa, using NBP1-31344 at 1:200 dilution.](http://images.novusbio.com/fullsize/PLA2G4A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31344-img0008.jpg)
![Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344] - PLA2G4A antibody detects PLA2G4A protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PLA2G4A antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PLA2G4A-Antibody-Immunohistochemistry-Paraffin-NBP1-31344-img0013.jpg)
![Western Blot: PLA2G4A Antibody [NBP1-31344] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PLA2G4A-Antibody-Western-Blot-NBP1-31344-img0010.jpg)
![Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344] - U87 xenograft, using PLA2G4A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PLA2G4A-Antibody-Immunohistochemistry-Paraffin-NBP1-31344-img0009.jpg)
![Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-31344] - PLA2G4A antibody detects PLA2G4A protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum.PLA2G4A antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PLA2G4A-Antibody-Immunohistochemistry-Paraffin-NBP1-31344-img0012.jpg)
![Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Western-Blot-MAB9355-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions")
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - An intracellular stain was performed on MCF7 cells with HSP27 [p Ser78] Antibody MAB9355 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Flow-Cytometry-MAB9355-img0003.jpg)
![Simple Western: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Lane vies shows a specific band for HSP27 using 0.2 mg/mL MCF-7 -/+ UV cell lysates and antibody at 5 ug/mL. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 32 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Simple-Western-MAB9355-img0004.jpg)
![Western Blot: IL-7R alpha/CD127 Antibody (22F3D9) [NBP2-22376] - Analysis of CD127 in A) a partial recombinant protein and B) Daudi lysate using CD127 antibody at 0.5 ug/ml and 5 ug/ml respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-22F3D9-Western-Blot-NBP2-22376-img0003.jpg "Western Blot: IL-7R alpha/CD127 Antibody (22F3D9) [NBP2-22376] - Analysis of CD127 in A) a partial recombinant protein and B) Daudi lysate using CD127 antibody at 0.5 ug/ml and 5 ug/ml respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: IL-7R alpha/CD127 Antibody (22F3D9) [NBP2-22376] - Analysis of IL-7 R alpha in human PMBCs using anti-IL-7 R alpha antibody. Image from verified customer review. Image using the Alexa Fluor 405 format of this antibody.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-22F3D9-Flow-Cytometry-NBP2-22376-img0011.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249383145.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-212024943559.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249403847.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249371547.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Human tissue extract (5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249415950.jpg)
![Synaptotagmin 1 antibody [HL1654] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-immunohistochemistry-paraffin-14112025183373.jpg)