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![Western Blot: VIPR1/VPAC1 Antibody (PD00-96) [NBP3-33097] - Western blot analysis of VIPR1/VPAC1 on different lysates with Rabbit anti-VIPR1/VPAC1 antibody (NBP3-33097) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: MDA-MB-231 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: LNCaP cell lysate (20 ug/Lane) Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33097) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33097_rabbit-vipr1-vpac1-mab-pd00-96-288202414191544.jpg "Western Blot: VIPR1/VPAC1 Antibody (PD00-96) [NBP3-33097] - Western blot analysis of VIPR1/VPAC1 on different lysates with Rabbit anti-VIPR1/VPAC1 antibody (NBP3-33097) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: MDA-MB-231 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: LNCaP cell lysate (20 ug/Lane) Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33097) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: VIPR1/VPAC1 Antibody (PD00-96) [NBP3-33097] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-VIPR1/VPAC1 antibody (NBP3-33097) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33097) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33097_rabbit-vipr1-vpac1-mab-pd00-96-298202414304187.jpg)
![Western Blot: RAPGEF5 Antibody [NBP2-57362] - Analysis in human cell line RT-4, human cell line U-251 MG and human plasma.](http://images.novusbio.com/fullsize/RAPGEF5-Antibody-Western-Blot-NBP2-57362-img0002.jpg "Western Blot: RAPGEF5 Antibody [NBP2-57362] - Analysis in human cell line RT-4, human cell line U-251 MG and human plasma.")
![Immunocytochemistry/Immunofluorescence: RAPGEF5 Antibody [NBP2-57362] - Staining of human cell line PC-3 shows localization to nucleoplasm & nuclear bodies.](http://images.novusbio.com/fullsize/RAPGEF5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57362-img0001.jpg)
![Western Blot: RAPGEF5 Antibody [H00009771-B01P] - Analysis of RAPGEF5 expression in transfected 293T cell line by RAPGEF5 polyclonal antibody. Lane 1: RAPGEF5 transfected lysate(48.95 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RAPGEF5-Antibody-Western-Blot-H00009771-B01P-img0004.jpg "Western Blot: RAPGEF5 Antibody [H00009771-B01P] - Analysis of RAPGEF5 expression in transfected 293T cell line by RAPGEF5 polyclonal antibody. Lane 1: RAPGEF5 transfected lysate(48.95 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RAPGEF5 Antibody [H00009771-B01P] - Analysis of purified antibody to RAPGEF5 on HepG2 cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/RAPGEF5-Antibody-Immunocytochemistry-Immunofluorescence-H00009771-B01P-img0003.jpg)
![ELISA: Human VIPR1/VPAC1 ELISA Kit (Colorimetric) [NBP2-82391] - Samples were spiked with high concentrations of Human VIPR1/VPAC1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82391_human-vipr1-vpac1-elisa-kit-colorimetric-13220241626430.jpg "ELISA: Human VIPR1/VPAC1 ELISA Kit (Colorimetric) [NBP2-82391] - Samples were spiked with high concentrations of Human VIPR1/VPAC1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human VIPR1/VPAC1 ELISA Kit (Colorimetric) [NBP2-82391] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82391_human-vipr1-vpac1-elisa-kit-colorimetric-432024973655.jpg)
![Immunohistochemistry: RAPGEF5 Antibody [NBP2-38835] - Staining of human spleen shows distinct nuclear positivity in cells of the spleen.](http://images.novusbio.com/fullsize/RAPGEF5-Antibody-Immunohistochemistry-NBP2-38835-img0001.jpg "Immunohistochemistry: RAPGEF5 Antibody [NBP2-38835] - Staining of human spleen shows distinct nuclear positivity in cells of the spleen.")
![Western Blot: RAPGEF5 Antibody [NBP1-32220] - Sample(30 ug of whole cell lysate)A:HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RAPGEF5-Antibody-Western-Blot-NBP1-32220-img0002.jpg "Western Blot: RAPGEF5 Antibody [NBP1-32220] - Sample(30 ug of whole cell lysate)A:HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:1000.")
![Western Blot: RAPGEF5 Antibody - BSA Free [NBP3-46797] - Human brain tissue were subjected to SDS PAGE followed by western blot with (RAPGEF5 antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-46797_rabbit-rapgef5-pab-bsa-free-284202512283378.jpg "Western Blot: RAPGEF5 Antibody - BSA Free [NBP3-46797] - Human brain tissue were subjected to SDS PAGE followed by western blot with (RAPGEF5 antibody) at dilution of 1:500")
![Western Blot: VIPR1/VPAC1 Antibody [NBP2-94168] - Analysis of extracts of Raji cells, using VIPR1/VPAC1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/VIPR1-VPAC1-Antibody-Western-Blot-NBP2-94168-img0001.jpg "Western Blot: VIPR1/VPAC1 Antibody [NBP2-94168] - Analysis of extracts of Raji cells, using VIPR1/VPAC1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.")
![Western Blot: RAPGEF5 Overexpression Lysate (Adult Normal) [NBP2-07464] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAPGEF5.](http://images.novusbio.com/fullsize/RAPGEF5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07464-img0001.jpg "Western Blot: RAPGEF5 Overexpression Lysate (Adult Normal) [NBP2-07464] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAPGEF5.")
![Immunohistochemistry-Paraffin: VIPR1/VPAC1 Antibody [NLS1297] - Analysis of anti-VIP Receptor 1 antibody with human adipocytes.](http://images.novusbio.com/fullsize/VIPR1-VPAC1-Antibody-Immunohistochemistry-Paraffin-NLS1297-img0002.jpg "Immunohistochemistry-Paraffin: VIPR1/VPAC1 Antibody [NLS1297] - Analysis of anti-VIP Receptor 1 antibody with human adipocytes.")
![Immunohistochemistry: VIPR1/VPAC1 Antibody - BSA Free [NBP3-45597] - Immunohistochemistry of paraffin-embedded human kidney tissue slide using (VIPR1/VPAC1 Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-45597_rabbit-vipr1-vpac1-pab-bsa-free-284202512582696.jpg "Immunohistochemistry: VIPR1/VPAC1 Antibody - BSA Free [NBP3-45597] - Immunohistochemistry of paraffin-embedded human kidney tissue slide using (VIPR1/VPAC1 Antibody) at dilution of 1:50")
![Western Blot: VIP R1/VPAC1 Antibody [H00007433-B01P] - Analysis of VIPR1 expression in transfected 293T cell line by VIPR1 polyclonal antibody. Lane 1: VIPR1 transfected lysate(50.27 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/VIP-R1-VPAC1-Antibody-Western-Blot-H00007433-B01P-img0003.jpg "Western Blot: VIP R1/VPAC1 Antibody [H00007433-B01P] - Analysis of VIPR1 expression in transfected 293T cell line by VIPR1 polyclonal antibody. Lane 1: VIPR1 transfected lysate(50.27 KDa). Lane 2: Non-transfected lysate.")