| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42190-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05122-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-644SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97814-50ul | |||
![Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190] - Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa Lgi1 protein using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Load: 10 ug. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Western-Blot-NBP2-42190-img0003.jpg "Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190] - Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa Lgi1 protein using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Load: 10 ug. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.")
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Lgi1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Lgi1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0005.jpg)
![Western Blot: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB9.](http://images.novusbio.com/fullsize/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Western-Blot-NBP2-72918-img0002.jpg "Western Blot: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB9.")
![Flow Cytometry: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - Analysis of Jurkat cells, using anti-NDUFB9 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72918-img0004.jpg)
![Western Blot: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NDUFB9 monoclonal antibody.](http://images.novusbio.com/fullsize/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Western-Blot-NBP2-72918-img0001.jpg)
![Flow Cytometry: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - Analysis of Hela cells, using anti-NDUFB9 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72918-img0003.jpg)
![Western Blot: ITM2B Antibody (OTI1C11) - Azide and BSA Free [NBP2-71460] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITM2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITM2B.](http://images.novusbio.com/fullsize/ITM2B-Antibody-OTI1C11-Azide-and-BSA-Free-Western-Blot-NBP2-71460-img0001.jpg "Western Blot: ITM2B Antibody (OTI1C11) - Azide and BSA Free [NBP2-71460] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITM2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITM2B.")
![Immunohistochemistry: ITM2B Antibody (OTI1C11) - Azide and BSA Free [NBP2-71460] - Staining of paraffin-embedded Human Kidney tissue using anti-ITM2B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ITM2B-Antibody-OTI1C11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71460-img0004.jpg)
![Flow Cytometry: ITM2B Antibody (OTI1C11) - Azide and BSA Free [NBP2-71460] - Analysis of Jurkat cells, using anti-ITM2B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ITM2B-Antibody-OTI1C11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71460-img0003.jpg)
![Flow Cytometry: ITM2B Antibody (OTI1C11) - Azide and BSA Free [NBP2-71460] - Analysis of Hela cells, using anti-ITM2B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ITM2B-Antibody-OTI1C11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71460-img0002.jpg)
![Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05122-img0011.jpg "Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.")
![Western Blot: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Lane 1: purified C3, lane 2: purifed C3 reduced/DTT, lane 3: purified C3a , lane 4: EDTA-plasma. Lane 5: zymosan-activated serum](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Western-Blot-NBP1-05122-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining of human kidney. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05122-img0007.jpg)
![Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Sandwich-ELISA-Capture-NBP1-05122-img0005.jpg)
![Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB100-644-img0002.jpg "Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.")
![Simple Western: PINK1 Antibody [NB100-644] - Simple Western lane view shows a specific band for PINK1 at a dilution of 1:50 in 1.0 mg/ml of HeLa lysate. Molecular weight ~56 kDa. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PINK1-Antibody-Simple-Western-NB100-644-img0003.jpg)
![Immunohistochemistry: CNPase Antibody [NB100-1935] - A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-NB100-1935-img0001.jpg "Immunohistochemistry: CNPase Antibody [NB100-1935] - A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.")
![CNPase Antibody [NB100-1935] - CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.](http://images.novusbio.com/fullsize/CNPase-Antibody-NB100-1935-img0002.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32388) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-27820241631677.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32388) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32388) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-298202414161846.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-29202410234266.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32388, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-2920241645329.jpg)
![Western Blot: IP3R1 Antibody [NBP3-09066] - Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Western-Blot-NBP3-09066-img0001.jpg "Western Blot: IP3R1 Antibody [NBP3-09066] - Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.")
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP3-09066] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IP3 Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP3-09066-img0003.jpg)
![Flow Cytometry: IP3R1 Antibody [NBP3-09066] - Flow cytometric analysis of SH-SY-5Y cells with IP3 Receptor antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Flow-Cytometry-NBP3-09066-img0004.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP3-09066] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IP3 Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP3-09066-img0002.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32389) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-278202415212487.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32389) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-2982024149266.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-292024119233.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32389, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-29202416372194.jpg)
![Western Blot: WWOX Antibody [NBP2-97814] - Anti-WWOX rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lane B: Mouse brain tissue lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-97814-img0003.jpg "Western Blot: WWOX Antibody [NBP2-97814] - Anti-WWOX rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lane B: Mouse brain tissue lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa")
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-97814] - Immunochemical staining of human WWOX in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-97814-img0004.jpg)
![Immunoprecipitation: WWOX Antibody [NBP2-97814] - WWOX was immunoprecipitated using: Lane A: 0.5 mg U251MG Whole Cell Lysate 4 ul anti-WWOX rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-WWOX rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunoprecipitation-NBP2-97814-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP2-97814] - Immunofluorescence staining of WWOX in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97814 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97814_rabbit-polyclonal-wwox-antibody-235202521451771.png)
![Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: THP-1 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-288202412442993.jpg "Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: THP-1 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32860) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-298202414333682.jpg)
![Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling LEDGF with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32860, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-29820241420980.jpg)
![Immunocytochemistry/ Immunofluorescence: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunocytochemistry analysis of SH-SY5Y cells labeling LEDGF with Mouse anti-LEDGF antibody (NBP3-32860) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-LEDGF antibody (NBP3-32860) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-29202410234271.jpg)