| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB300-198-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61710-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22397-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41364-0.025mg | |||
![Western Blot: CNPase Antibody [NB110-93362] - Rat brain lysate showing the specific immunolabeling of the ~ 46k CNP protein.](http://images.novusbio.com/fullsize/CNPase-Antibody-Western-Blot-NB110-93362-img0003.jpg "Western Blot: CNPase Antibody [NB110-93362] - Rat brain lysate showing the specific immunolabeling of the ~ 46k CNP protein.")
![Immunohistochemistry-Frozen: CNPase Antibody [NB110-93362] - Staining of saline treated mouse cortex cryosections with CNPase antibody (green). Cell nuclei are visualized with DAPI DNA stain (blue).](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-Frozen-NB110-93362-img0004.jpg)
![Western Blot: APP Antibody [NB300-308]](http://images.novusbio.com/fullsize/APP-Antibody-Western-Blot-NB300-308-img0001.jpg "Western Blot: APP Antibody [NB300-308]")
![Immunocytochemistry/Immunofluorescence: APP Antibody [NB300-308] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) APP. Overlay images indicate the possible co-localization of VDR/APP on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-308-img0002.jpg)
![Western Blot: APP Antibody [NB300-308] - Cell surface staining of VDR (green) on live neurons followed by fixation & immunofluorescence labeling of target proteins (100x): A) ADAM10; B) APP; C) BACE1; D) Nicastrin; E) Presenilin 1; F) Presenilin 2. Overlay images indicate the possible co-localization of VDR/ADAM10 (A) or VDR/APP (B) or VDR/Nicastrin (D) or limited co-localization of VDR/ Presenilin 1 (E) on the neuronal plasma membrane. Overlay images do not indicate co-localization for VDR/BACE1 (C) or VDR/Presenilin 2 (F) on the neuronal plasma membrane. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29176823), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-308_rabbit-polyclonal-app-antibody-31020241533495.jpg)
![Western Blot: GABA-AR alpha 1 Antibody [NB300-207] - Mouse forebrain lysates from wild type (Control) and a1-knockout (a1-K/O) animals showing specific immunolabeling of the ~51k a1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a1-knockout animals.](http://images.novusbio.com/fullsize/GABA-AR-alpha-1-Antibody-Western-Blot-NB300-207-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GABA-AR alpha 1 Antibody [NB300-207] - Mouse forebrain lysates from wild type (Control) and a1-knockout (a1-K/O) animals showing specific immunolabeling of the ~51k a1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a1-knockout animals.")
![Western Blot: GABA-A R beta 2 Antibody [NB300-198] - 7 ug of rat cerebellum (Cb) showing specific immunolabeling of the ~55k beta2-subunit of the GABA-AR.](http://images.novusbio.com/fullsize/GABA-A-R-beta-2-Antibody-Western-Blot-NB300-198-img0004.jpg "Western Blot: GABA-A R beta 2 Antibody [NB300-198] - 7 ug of rat cerebellum (Cb) showing specific immunolabeling of the ~55k beta2-subunit of the GABA-AR.")
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mAb against HEK293 (1) and ADRB2 (AA: 302-413)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Western-Blot-NBP2-61710-img0004.jpg "Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mAb against HEK293 (1) and ADRB2 (AA: 302-413)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis of MCF-7 cells using ADRB2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Flow-Cytometry-NBP2-61710-img0002.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mAb against human ADRB2 (AA: 302-413) recombinant protein. (Expected MW is 38.5 kDa)](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Western-Blot-NBP2-61710-img0003.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mouse mAb against C6 (1) cell lysate.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Western-Blot-NBP2-61710-img0005.jpg)
![ELISA: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-ELISA-NBP2-61710-img0001.jpg)
![Western Blot: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Alpha-2C showing detection of ~50 kDa Alpha-2C Adrenergic Receptor protein using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Alpha-2C. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.](http://images.novusbio.com/fullsize/alpha-2C-Adrenergic-R-ADRA2C-Antibody-S330A-80-Western-Blot-NBP2-22397-img0003.jpg "Western Blot: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Alpha-2C showing detection of ~50 kDa Alpha-2C Adrenergic Receptor protein using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Alpha-2C. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Alpha-2C Adrenergic Receptor Antibody (D) Composite.](http://images.novusbio.com/fullsize/alpha-2C-Adrenergic-R-ADRA2C-Antibody-S330A-80-Immunocytochemistry-Immunofluorescence-NBP2-22397-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/alpha-2C-Adrenergic-R-ADRA2C-Antibody-S330A-80-Immunocytochemistry-Immunofluorescence-NBP2-22397-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha-2C Adrenergic Receptor Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-2C-Adrenergic-R-ADRA2C-Antibody-S330A-80-Immunocytochemistry-Immunofluorescence-NBP2-22397-img0005.jpg)
![Western Blot: DARPP32 Antibody [NB300-304] - Detection of DARPP protein in rat hippocampal lysate using NB 300-304.](http://images.novusbio.com/fullsize/DARPP32-Antibody-Western-Blot-NB300-304-img0001.jpg "Western Blot: DARPP32 Antibody [NB300-304] - Detection of DARPP protein in rat hippocampal lysate using NB 300-304.")
![Immunocytochemistry/Immunofluorescence: DARPP-32 Antibody [NB300-304] - Staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 in green and Tuj1 in red. Cells and photo courtesy of QBMCellScience.](http://images.novusbio.com/fullsize/DARPP-32-Antibody-Immunocytochemistry-Immunofluorescence-NB300-304-img0003.jpg)
![Immunohistochemistry: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364]](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-14-BSA-Free-Immunohistochemistry-NBP2-41364-img0005.jpg "Immunohistochemistry: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364]")
![Sandwich ELISA: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364] - Analysis using the Biotin conjugate of NBP2-41364. The calibration curveusing NBP2-23595 as the biotinylated detection antibody and NBP2-41364 as the capture antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-14-BSA-Free-Sandwich-ELISA-NBP2-41364-img0004.jpg)
![Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-278202415295526.jpg "Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-iNOS antibody (NBP3-32473) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32473) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-298202414304151.jpg)
![Immunocytochemistry/ Immunofluorescence: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunocytochemistry analysis of RAW264.7 cells treated with or without 1μg/mL LPS for 6 hours labeling iNOS with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-29202410334018.jpg)
![Western Blot: CPT1A Antibody (PSH04-00) [NBP3-32213] - Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: SK-OV-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: Human kidney tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32213) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-278202416285228.jpg "Western Blot: CPT1A Antibody (PSH04-00) [NBP3-32213] - Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: SK-OV-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: Human kidney tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32213) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32213) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-29820241435278.jpg)
![Flow Cytometry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Flow cytometric analysis of HepG2 cells labeling CPT1A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32213, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-29202416453278.jpg)
![Immunohistochemistry-Paraffin: mGluR4 Antibody [NLS4713] - Anti-MGLUR4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 19 ug/ml.](http://images.novusbio.com/fullsize/mGluR4-Antibody-Immunohistochemistry-Paraffin-NLS4713-img0002.jpg "Immunohistochemistry-Paraffin: mGluR4 Antibody [NLS4713] - Anti-MGLUR4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 19 ug/ml.")
![Immunohistochemistry-Paraffin: mGluR4 Antibody [NLS4713] - Analysis of anti-MGLUR4 antibody with human brain, cortex, neurons at dilution 19 ug/ml.](http://images.novusbio.com/fullsize/mGluR4-Antibody-Immunohistochemistry-Paraffin-NLS4713-img0001.jpg)
![Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-27820241631618.jpg "Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32584) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-298202414304162.jpg)
![Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunocytochemistry analysis of U-2 OS cells treated with or without 10μM NUTLIN 3A for 24 hours labeling MDM2/HDM2 with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-29202410484835.jpg)