| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87103-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89820-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-89182SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33555-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44099 | |||
| BSA Free | NBP2-59057-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32448-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70857 | |||
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0016.jpg "Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0015.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0007.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human heart muscle shows moderate to strong nuclear positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining in human heart muscle and lymph node tissues.. Corresponding S100A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0012.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human lymph node shows no positivity in non - germinal center cells as expected.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0013.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human skin shows moderate to strong cytoplasmic positivity in melanocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Frequenin Antibody [NBP1-89820] - Staining of human cell line U-2 OS shows localization to plasma membrane and vesicles.](http://images.novusbio.com/fullsize/Frequenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89820-img0013.jpg "Immunocytochemistry/Immunofluorescence: Frequenin Antibody [NBP1-89820] - Staining of human cell line U-2 OS shows localization to plasma membrane and vesicles.")
![Immunohistochemistry-Paraffin: Frequenin Antibody [NBP1-89820] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Frequenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89820-img0010.jpg)
![Immunohistochemistry-Paraffin: Frequenin Antibody [NBP1-89820] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Frequenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89820-img0011.jpg)
![Western Blot: Frequenin Antibody [NBP1-89820] - Analysis in control (vector only transfected HEK293T lysate) and NCS1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Frequenin-Antibody-Western-Blot-NBP1-89820-img0014.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-89820_rabbit-polyclonal-frequenin-antibody-15520258574147.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-89820_rabbit-polyclonal-frequenin-antibody-15520258273033.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249415924.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-212024935136.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249432872.jpg)
![Immunohistochemistry-Frozen: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:300. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249393591.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249415945.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249342788.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249374965.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NBP2-89182-img0003.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-89182-img0002.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of NPC1L1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NPC1L1 antibody generated moderate staining of hepatocyte cytoplasm.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NBP2-89182-img0001.jpg)
![Western Blot: MBP Antibody [NBP2-33555] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NBP2-33555-img0014.jpg "Western Blot: MBP Antibody [NBP2-33555] - Analysis in mouse cerebral cortex tissue.")
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse olfactory bulb shows immunoreactivity in processes in external plexiform layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0011.jpg)
![Western Blot: MBP Antibody [NBP2-33555] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NBP2-33555-img0013.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human cerebral cortex shows positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0005.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human hippocampus shows moderate immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0006.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human cerebellum shows moderate nuclear and cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0015.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse cerebellum shows immunoreactivity in granular cell layer and in white matter.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0007.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse cerebral cortex shows labeling of neuronal processes in motor cortex and in corpus callosum.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0008.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse dorsal tenia tecta shows positivity in neuronal processes.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0009.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse hippocampus shows positivity in neuronal processes in the CA1 layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0010.jpg)
![Western Blot: SOX2 Antibody (CL4716) [NBP2-59057] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SOX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Western-Blot-NBP2-59057-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SOX2 Antibody (CL4716) [NBP2-59057] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SOX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Western Blot: SOX2 Antibody (CL4716) [NBP2-59057] - Analysis in human cell line NTERA-2.](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Western-Blot-NBP2-59057-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SOX2 Antibody (CL4716) [NBP2-59057] - Staining of U-251 cells using the anti-SOX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Immunocytochemistry-Immunofluorescence-NBP2-59057-img0001.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (CL4716) [NBP2-59057] - Staining of human placenta shows no positivity as expected (negative control).](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Immunohistochemistry-Paraffin-NBP2-59057-img0005.jpg)
![Immunohistochemistry: SOX2 Antibody (CL4716) [NBP2-59057] - SOX2 Antibody [NBP2-59057] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing brain, eye and trigeminal ganglion.](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Immunohistochemistry-NBP2-59057-img0007.jpg)
![Immunohistochemistry: SOX2 Antibody (CL4716) [NBP2-59057] - SOX2 Antibody [NBP2-59057] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing neural tube.](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Immunohistochemistry-NBP2-59057-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: DTX4 Antibody [NBP2-32448] - Staining of mouse pons shows strong cytoplasmic positivity in neurons of the motor trigeminal nucleus.](http://images.novusbio.com/fullsize/DTX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32448-img0009.jpg "Immunocytochemistry/Immunofluorescence: DTX4 Antibody [NBP2-32448] - Staining of mouse pons shows strong cytoplasmic positivity in neurons of the motor trigeminal nucleus.")
![Immunohistochemistry-Paraffin: DTX4 Antibody [NBP2-32448] - Staining of human hippocampus shows moderate granular immunoreactivity in neuronal cell bodies.](http://images.novusbio.com/fullsize/DTX4-Antibody-Immunohistochemistry-Paraffin-NBP2-32448-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DTX4 Antibody [NBP2-32448] - Staining of mouse hippocampus shows restricted positivity in the CA2/CA3 areas.](http://images.novusbio.com/fullsize/DTX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32448-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DTX4 Antibody [NBP2-32448] - Staining of mouse olfactory bulb shows weak staining in the external plexiform layer.](http://images.novusbio.com/fullsize/DTX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32448-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DTX4 Antibody [NBP2-32448] - Staining of mouse pons shows cytoplasmic staining in the vestibular nucleus.](http://images.novusbio.com/fullsize/DTX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32448-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: DTX4 Antibody [NBP2-32448] - Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.](http://images.novusbio.com/fullsize/DTX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32448-img0010.jpg)
![Immunohistochemistry: DTX4 Antibody [NBP2-32448] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DTX4-Antibody-Immunohistochemistry-NBP2-32448-img0001.jpg)
![Immunohistochemistry-Paraffin: DTX4 Antibody [NBP2-32448] - Staining of human cerebellum shows strong granular cytoplasmic immunoreactivity in Purkinje cells.](http://images.novusbio.com/fullsize/DTX4-Antibody-Immunohistochemistry-Paraffin-NBP2-32448-img0004.jpg)
![Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Western-Blot-NBP3-13575-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI/Quaking protein stained by QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Frozen-NBP3-13575-img0005.jpg)
![Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:3000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Western-Blot-NBP3-13575-img0001.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Paraffin-NBP3-13575-img0002.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Paraffin-NBP3-13575-img0003.jpg)
![QKI antibody [GT1589] detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: QKI protein stained by QKI antibody [GT1589] (NBP3-13575) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp3-13575_mouse-qki-quaking-mab-gt1589-immunocytochemistry-immunofluorescence-2611202518235322.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-2120249413328.jpg "Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Genetic Strategies Validation. </b>Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-2120249425474.jpg)
![Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-212024942544.jpg)
![Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-21202494142.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-2120249432896.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-212024938315.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-212024937492.jpg)
![Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Frozen-NBP3-13014-img0008.jpg "Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein in mouse retina by immunohistochemical analysis. Sample: Paraffin-embedded mouse retina. HCN1 antibody (NBP3-13014) diluted at 1:800. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0002.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HCN1 antibody (NBP3-13014) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0003.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HCN1 antibody (NBP3-13014) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0004.jpg)
![Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Frozen-NBP3-13014-img0005.jpg)
![HCN1 antibody detects HCN1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HCN1 stained by HCN1 antibody (NBP3-13014) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13014_rabbit-hcn1-pab-immunocytochemistry-immunofluorescence-261120251818921.jpg)
![Immunohistochemistry-Paraffin: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-212024940820.jpg "Immunohistochemistry-Paraffin: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:250. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-2120249425467.jpg)
![Western Blot: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-2120249422617.jpg)
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1739] (1 ug/mL)Please notice that NBP3-25728 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637387. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-1411202518443414.jpg)
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human TREM2 protein (1215.95-19.07 pM). Coated protein was probed with TREM2 antibody [HL1738] (NBP3-25728) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-1411202518414124.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-western-blot-14112025184488.jpg)
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1738] (NBP3-25728) (5 ug/mL) Detection: HRP-conjugated TREM2 antibody [HL1739] (1 ug/mL)Please notice that GTX637387 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25728. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-141120251843335.jpg)
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSTP1.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-4B6-Western-Blot-NBP2-45916-img0010.jpg "Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSTP1.")
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-4B6-Immunohistochemistry-NBP2-45916-img0009.jpg)
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-4B6-Immunohistochemistry-NBP2-45916-img0001.jpg)
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-4B6-Immunohistochemistry-NBP2-45916-img0002.jpg)
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-4B6-Immunohistochemistry-NBP2-45916-img0003.jpg)
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-4B6-Immunohistochemistry-NBP2-45916-img0004.jpg)
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-4B6-Immunohistochemistry-NBP2-45916-img0005.jpg)
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-4B6-Immunohistochemistry-NBP2-45916-img0006.jpg)
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-4B6-Immunohistochemistry-NBP2-45916-img0007.jpg)
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (4B6) [NBP2-45916] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-4B6-Immunohistochemistry-NBP2-45916-img0008.jpg)