| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05533-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02943-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59603-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21673-50ul | |||
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Western-Blot-NB100-40805-img0004.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Frozen: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of PFA-perfused cryosection of Porcine Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Immunohistochemistry-Frozen-NB100-40805-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Immunohistochemistry-Paraffin-NB100-40805-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Immunohistochemistry-Paraffin-NB100-40805-img0002.jpg)
![Western Blot: LIN-28A Antibody [NBP2-19355] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Western-Blot-NBP2-19355-img0008.jpg "Western Blot: LIN-28A Antibody [NBP2-19355] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE gel, diluted at 1:3000.")
![Immunohistochemistry-Paraffin: LIN-28A Antibody [NBP2-19355] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunohistochemistry-Paraffin-NBP2-19355-img0006.jpg)
![Flow Cytometry: LIN-28A Antibody [NBP2-19355] - Human embryonic stem cells, staining with LIN28 antibody at 1:100 dilution (purple) or rabbit IgG (black).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Flow-Cytometry-NBP2-19355-img0005.jpg)
![Western Blot: LIN-28A Antibody [NBP2-19355] - Sample (20 ug of whole cell lysate) A: hESC 12% SDS PAGE NBP2-19355 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19355_rabbit-polyclonal-lin-28a-antibody-910202420134044.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - Fibrillarin antibody immunoprecipitates FBL protein in IP experiments. IP samples: HeLa nuclear extract. A. 35 ug HeLa nuclear extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of FBL protein by 4 ug Fibrillarin antibody. 10 % SDS-PAGE. The immunoprecipitated FBL protein was detected by Fibrillarin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0005.jpg "Western Blot: Fibrillarin Antibody [NBP2-16492] - Fibrillarin antibody immunoprecipitates FBL protein in IP experiments. IP samples: HeLa nuclear extract. A. 35 ug HeLa nuclear extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of FBL protein by 4 ug Fibrillarin antibody. 10 % SDS-PAGE. The immunoprecipitated FBL protein was detected by Fibrillarin antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-16492] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibrillarin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16492-img0004.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - 30 ug Rat whole cell lysate/extract 12 % SDS-PAGE Fibrillarin antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0001.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0002.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0003.jpg)
![Western Blot: Fibrillarin Antibody [NBP3-05533] - Western blot analysis of different tissue and cell line lysates using Fibrillarin Antibody at a dilution of 1:2,000, in green: [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds to Fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since Fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with rabbit pAb to UCHL1 at a dilution of 1:3,000, in red, revealing the ~25kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP3-05533-img0002.jpg "Western Blot: Fibrillarin Antibody [NBP3-05533] - Western blot analysis of different tissue and cell line lysates using Fibrillarin Antibody at a dilution of 1:2,000, in green: [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds to Fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since Fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with rabbit pAb to UCHL1 at a dilution of 1:3,000, in red, revealing the ~25kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP3-05533] - Immunofluorescent analysis of (A) C6 rat glioma cells and (B) HEK293 human embryonic kidney cells stained with Fibrillarin Antibody at a dilution of 1:1,000 in red, in both cases costained with chicken pAb to vimentin at a dilution of 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The Fibrillarin antibody detects Fibrillarin protein localized in nucleoli while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05533-img0001.jpg)
![Western Blot: MT-ND2 Antibody [NBP3-05589] - Western blot analysis of extracts of various cell lines, using MT-ND2 antibody (NBP3-05589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MT-ND2 Antibody-Western Blot-NBP3-05589-img0003.jpg "Western Blot: MT-ND2 Antibody [NBP3-05589] - Western blot analysis of extracts of various cell lines, using MT-ND2 antibody (NBP3-05589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: MT-ND2 Antibody [NBP3-05589] - Immunofluorescence analysis of HeLa cells using MT-ND2 antibody (NBP3-05589) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MT-ND2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05589-img0001.jpg)
![Immunohistochemistry-Paraffin: MT-ND2 Antibody [NBP3-05589] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MT-ND2 Rabbit pAb (NBP3-05589) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MT-ND2-Antibody-Immunohistochemistry-Paraffin-NBP3-05589-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: MT-ND2 Antibody - Azide and BSA Free [MT-ND2] - Immunofluorescence analysis of HeLa cells using MT-ND2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05589_rabbit-polyclonal-mt-nd2-antibody-5220251718985.jpg)
![Western Blot: HuC Antibody - Azide and BSA Free [HuC] - Western blot analysis of various lysates, using HuC Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.8s.](http://images.novusbio.com/fullsize/nbp3-02943_rabbit-huc-pab-52202516321480.jpg "Western Blot: HuC Antibody - Azide and BSA Free [HuC] - Western blot analysis of various lysates, using HuC Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.8s.")
![Immunohistochemistry-Paraffin: HuC Antibody [NBP3-02943] - Immunohistochemistry of paraffin-embedded mouse brain using HuC antibody (NBP3-02943) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HuC-Antibody-Immunohistochemistry-Paraffin-NBP3-02943-img0006.jpg)
![Immunohistochemistry-Paraffin: HuC Antibody [NBP3-02943] - Paraffin-embedded rat brain using HuC antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/HuC-Antibody-Immunohistochemistry-Paraffin-NBP3-02943-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: HuC Antibody - Azide and BSA Free [HuC] - Immunofluorescence analysis of paraffin-embedded mouse brain using HuC Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02943_rabbit-huc-pab-52202516321493.jpg)
![Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843] - Western blot analysis of extracts of various cell lines, using LIN-28A antibody (NBP3-16843) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-2S1I10-Western-Blot-NBP3-16843-img0004.jpg "Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843] - Western blot analysis of extracts of various cell lines, using LIN-28A antibody (NBP3-16843) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of mouse testis using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-2S1I10-Immunohistochemistry-NBP3-16843-img0003.jpg)
![Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of rat testis using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-2S1I10-Immunohistochemistry-NBP3-16843-img0001.jpg)
![Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of human ovary using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-2S1I10-Immunohistochemistry-NBP3-16843-img0002.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32388) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-27820241631677.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32388) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32388) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-298202414161846.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-29202410234266.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32388, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-2920241645329.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32389) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-278202415212487.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32389) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-2982024149266.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-292024119233.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32389, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-29202416372194.jpg)
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Rat Heart stained withNSE gamma Monoclonal Antibody (ENO2/1462).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Immunohistochemistry-Paraffin-NBP2-59602-img0009.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Rat Heart stained withNSE gamma Monoclonal Antibody (ENO2/1462).")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained withNSE gamma Monoclonal Antibody (ENO2/1462).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Immunohistochemistry-Paraffin-NBP2-59602-img0007.jpg)
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained withNSE gamma Monoclonal Antibody (ENO2/1462)](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Immunohistochemistry-Paraffin-NBP2-59602-img0008.jpg)
![Protein Array: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Protein-Array-NBP2-59602-img0012.jpg)
![Western Blot: Fibrillarin Antibody (SR1545) [NBP3-21673] - Western blot analysis using NBP3-21673 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21673_rabbit-fibrillarin-mab-sr1545-57202317215153.jpg "Western Blot: Fibrillarin Antibody (SR1545) [NBP3-21673] - Western blot analysis using NBP3-21673 in HepG2 cell lysate.")
![Immunohistochemistry: Fibrillarin Antibody (SR1545) [NBP3-21673] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21673.](http://images.novusbio.com/fullsize/nbp3-21673_rabbit-fibrillarin-mab-sr1545-5720231754866.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (SR1545) [NBP3-21673] - Immunofluorescent analysis of Hela cells, using NBP3-21673.](http://images.novusbio.com/fullsize/nbp3-21673_rabbit-fibrillarin-mab-sr1545-113202515145423.jpg)