| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92688SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47509-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49443-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87797-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47697-0.1mg | |||
| IHC-Prediluted | NBP2-48449 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41301-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03651-20ul | |||
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Knockout-Validated-NBP1-92688-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2A52) [NBP1-92688] - Immunocytochemistry/Immunofluorescence: [NBP1-92688] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Immunocytochemistry-Immunofluorescence-NBP1-92688-img0005.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Analysis of cell and whole brain tissue lysates using mouse mAb to vimentin, NBP1-92688, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] C6, and [7] rat brain. The band at about 50kDa mark corresponds to vimentin protein. The antibody does not recognize mouse vimentin (not shown).](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Western-Blot-NBP1-92688-img0006.jpg)
![Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Western-Blot-NBP2-27197-img0001.jpg "Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: SPR Antibody [NBP1-33546] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SPR-Antibody-Western-Blot-NBP1-33546-img0003.jpg "Western Blot: SPR Antibody [NBP1-33546] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SPR Antibody [NBP1-33546] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SPR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33546-img0002.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody [NBP1-33546] - Paraffin-embedded mouse fore brain. SPR antibody [N2C3] dilution: 1:500.](http://images.novusbio.com/fullsize/SPR-Antibody-Immunohistochemistry-Paraffin-NBP1-33546-img0004.jpg)
![Immunoprecipitation: SPR Antibody [NBP1-33546] - A431 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SPR protein by 4 ug SPR antibody [N2C3] 5 % SDS-PAGE The Immunoprecipitated SPR protein was detected by SPR antibody [N2C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent].](http://images.novusbio.com/fullsize/SPR-Antibody-Immunoprecipitation-NBP1-33546-img0006.jpg)
![Western Blot: NEDD4 Antibody [NBP2-27201] - Analysis of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27201. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Western-Blot-NBP2-27201-img0001.jpg "Western Blot: NEDD4 Antibody [NBP2-27201] - Analysis of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27201. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NBP2-47509] - Staining of human adrenal gland, colon, gallbladder and kidney using Anti-STUB1 antibody NBP2-47509 (A) shows similar protein distribution across tissues to independent antibody NBP2-47510 (B).](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Immunohistochemistry-Paraffin-NBP2-47509-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NBP2-47509] - Staining of human adrenal gland, colon, gallbladder and kidney using Anti-STUB1 antibody NBP2-47509 (A) shows similar protein distribution across tissues to independent antibody NBP2-47510 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody [NBP2-47509] - Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47509-img0003.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NBP2-47509] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Immunohistochemistry-Paraffin-NBP2-47509-img0007.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NBP2-47509] - Staining of human adrenal gland.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Immunohistochemistry-Paraffin-NBP2-47509-img0005.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NBP2-47509] - Staining of human colon.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Immunohistochemistry-Paraffin-NBP2-47509-img0006.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NBP2-47509] - Staining of human kidney.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Immunohistochemistry-Paraffin-NBP2-47509-img0008.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining in human skin and pancreas tissues using anti-UBA3 antibody. Corresponding UBA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining in human skin and pancreas tissues using anti-UBA3 antibody. Corresponding UBA3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: UBA3 Antibody [NBP2-49443] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-49443-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: UBA3 Antibody [NBP2-49443] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & centrosome.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49443-img0004.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0006.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0005.jpg)
![Western Blot: UBA3 Antibody [NBP2-48628] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-48628-img0002.jpg "Western Blot: UBA3 Antibody [NBP2-48628] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG")
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human testis shows strong nuclear positivity.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0007.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human cerebellum shows moderate nuclear positivity in purkinje cells.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0004.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0005.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human skin shows moderate nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0006.jpg)
![Western Blot: Proteasome 19S S7 Antibody [NBP1-87797] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Proteasome-19S-S7-Antibody-Western-Blot-NBP1-87797-img0008.jpg "Western Blot: Proteasome 19S S7 Antibody [NBP1-87797] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: Proteasome 19S S7 Antibody [NBP1-87797] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/Proteasome-19S-S7-Antibody-Immunohistochemistry-Paraffin-NBP1-87797-img0009.jpg)
![Simple Western: Proteasome 19S S7 Antibody [NBP1-87797] - Mouse whole spleen lysates at the indicated concentrations were probed with a 1:25 dilution of this Proteasome 19S S7 antibody. Burn-out of the major peak is beginning at 0.5 mg/mL. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Proteasome-19S-S7-Antibody-Simple-Western-NBP1-87797-img0010.jpg)
![Immunohistochemistry-Paraffin: SUMO1 Antibody (SUMO1/1188) [NBP2-44460] - Human Testicular Carcinoma stained with SUMO-1 Monoclonal Antibody (SUMO1/1188)](http://images.novusbio.com/fullsize/SUMO1-Antibody-SUMO1-1188-Immunohistochemistry-Paraffin-NBP2-44460-img0002.jpg "Immunohistochemistry-Paraffin: SUMO1 Antibody (SUMO1/1188) [NBP2-44460] - Human Testicular Carcinoma stained with SUMO-1 Monoclonal Antibody (SUMO1/1188)")
![Immunohistochemistry-Paraffin: SUMO1 Antibody (SUMO1/1188) [NBP2-44460] - Human Tonsil stained with SUMO-1 Monoclonal Antibody (SUMO1/1188)](http://images.novusbio.com/fullsize/SUMO1-Antibody-SUMO1-1188-Immunohistochemistry-Paraffin-NBP2-44460-img0003.jpg)
![Immunohistochemistry-Paraffin: SUMO1 Antibody (SUMO1/1188) [NBP2-44460] - Human Tonsil stained with SUMO-1 Monoclonal Antibody (SUMO1/1188)](http://images.novusbio.com/fullsize/SUMO1-Antibody-SUMO1-1188-Immunohistochemistry-Paraffin-NBP2-44460-img0004.jpg)
![Western Blot: KCNK1 Antibody [NBP2-41301] - Western blot analysis of KCNK1 in 3T3 cell lysate with KCNK1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/KCNK1-Antibody-Western-Blot-NBP2-41301-img0001.jpg "Western Blot: KCNK1 Antibody [NBP2-41301] - Western blot analysis of KCNK1 in 3T3 cell lysate with KCNK1 antibody at 1 ug/ml.")
![Immunohistochemistry: KCNK1 Antibody - BSA Free [NBP2-41301] - Immunohistochemistry of KCNK1 in mouse brain tissue with KCNK1 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp2-41301_rabbit-polyclonal-kcnk1-antibody-2711202419541567.jpg)
![Immunocytochemistry/ Immunofluorescence: KCNK1 Antibody - BSA Free [NBP2-41301] - Immunofluorescence of KCNK1 in mouse brain tissue with KCNK1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41301_rabbit-polyclonal-kcnk1-antibody-2711202419313249.jpg)
![Western Blot: FUS Antibody [NBP3-03651] - Analysis of extracts from normal (control) and FUS knockout (KO) 293T cells, using FUS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/FUS-Antibody-Knockout-Validated-NBP3-03651-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody [NBP3-03651] - Analysis of extracts from normal (control) and FUS knockout (KO) 293T cells, using FUS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: FUS Antibody [NBP3-03651] - Analysis of 300ug extracts of Jurkat cells using 3ug FUS antibody. Western blot was performed from the immunoprecipitate using FUS antibody at a dilition of 1:2000.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunoprecipitation-NBP3-03651-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: FUS Antibody - BSA Free [NBP3-03651] - Immunofluorescence analysis of C6 cells using [KO Validated] FUS Rabbit pAb (A5921) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03651_rabbit-polyclonal-fus-antibody-522025193132.jpg)
![Immunocytochemistry/ Immunofluorescence: FUS Antibody - BSA Free [NBP3-03651] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] FUS Rabbit pAb (A5921) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03651_rabbit-polyclonal-fus-antibody-52202519294534.jpg)
![Immunocytochemistry/Immunofluorescence: ATG12 Antibody (166) [NBP2-43781] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG12 protein stained by ATG12 antibody [166]. Red: Phalloidin, a F-actin marker.](http://images.novusbio.com/fullsize/ATG12-Antibody-166-Immunocytochemistry-Immunofluorescence-NBP2-43781-img0003.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATG12 Antibody (166) [NBP2-43781] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG12 protein stained by ATG12 antibody [166]. Red: Phalloidin, a F-actin marker.")
![<b>Biological Strategies Validation. </b>Western Blot: ATG12 Antibody (166) [NBP2-43781] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [166] dilution: 1:1000](http://images.novusbio.com/fullsize/ATG12-Antibody-166-Western-Blot-NBP2-43781-img0001.jpg)
![Western Blot: ATG12 Antibody (166) [NBP2-43781] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [166] dilution: 1:1000](http://images.novusbio.com/fullsize/ATG12-Antibody-166-Western-Blot-NBP2-43781-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT166] (NBP2-43781) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43781_mouse-monoclonal-atg12-antibody-166-western-blot-2611202518411322.jpg)