| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43408-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03477-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37937-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03894-20ul | |||
![Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408] - Staining of rat splenocytes with 0.5 ug of Mouse IgG2b K Isotype Control FITC (blue histogram) or 0.5 ug of Anti-Rat CD45R (B220) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis (left). Immunohistochemistry of frozen rat spleen using 10 ug/mL Anti-Rat CD45R (B220) FITC (green), 10 ug/mL Anti-Rat Marginal Zone B Cells eFluor 660 (white), and 10 ug/mL Anti-Rat CD3 Biotin followed by 1 ug/mL Streptavidin eFluor (R) 570 (red). Nuclei are stained with DAPI (blue)(right).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-HIS24-Immunohistochemistry-NBP1-43408-img0004.jpg "Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408] - Staining of rat splenocytes with 0.5 ug of Mouse IgG2b K Isotype Control FITC (blue histogram) or 0.5 ug of Anti-Rat CD45R (B220) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis (left). Immunohistochemistry of frozen rat spleen using 10 ug/mL Anti-Rat CD45R (B220) FITC (green), 10 ug/mL Anti-Rat Marginal Zone B Cells eFluor 660 (white), and 10 ug/mL Anti-Rat CD3 Biotin followed by 1 ug/mL Streptavidin eFluor (R) 570 (red). Nuclei are stained with DAPI (blue)(right).")
![Flow Cytometry: B220/CD45R Antibody (HIS24) [NBP1-43408] - Analysis using the FITC conjugate of NBP1-43408. Staining of rat splenocytes with 0.05 ug of Mouse IgG2b K Isotype Control FITC (open histogram) or 0.05 ug of Anti-Rat CD45R (B220) FITC (filled histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-HIS24-Flow-Cytometry-NBP1-43408-img0003.jpg)
![Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB100-61654-img0002.jpg "Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Pax6 Antibody [NB100-61654] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Flow-Cytometry-NB100-61654-img0003.jpg)
![Western Blot: Pax6 Antibody [NB100-61654] - (0.3ug/ml) staining of U251 (A), (0.03ug/ml) nuclear K562 (B) and (1ug/ml) nuclear HeLa (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB100-61654-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: xCT Antibody [NB100-59735] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells,permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/xCT-Antibody-Immunocytochemistry-Immunofluorescence-NB100-59735-img0001.jpg "Immunocytochemistry/Immunofluorescence: xCT Antibody [NB100-59735] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells,permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunocytochemistry/Immunofluorescence: xCT Antibody [NB100-59735] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/xCT-Antibody-Immunocytochemistry-Immunofluorescence-NB100-59735-img0002.jpg)
![Western Blot: GRAF Antibody [NB100-1327] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GRAF-Antibody-Western-Blot-NB100-1327-img0002.jpg "Western Blot: GRAF Antibody [NB100-1327] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: GRAF Antibody [NB100-1327] - staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/GRAF-Antibody-Immunohistochemistry-Paraffin-NB100-1327-img0003.jpg)
![Western Blot: HAP1 Antibody [NB100-55413] - Staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/HAP1-Antibody-Western-Blot-NB100-55413-img0001.jpg "Western Blot: HAP1 Antibody [NB100-55413] - Staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: HAP1 Antibody [NB100-55413] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HAP1-Antibody-Immunohistochemistry-Paraffin-NB100-55413-img0002.jpg)
![Western Blot: Synaptophysin Antibody [NBP1-59660] - Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Transfected 293T](http://images.novusbio.com/fullsize/synaptophysin-Antibody-Western-Blot-NBP1-59660-img0001.jpg "Western Blot: Synaptophysin Antibody [NBP1-59660] - Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Transfected 293T")
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NBP1-59660] - rat and mouse brain, 10 ug/ml. Observed staining in cytoplasmin neurons](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NBP1-59660-img0003.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NBP1-59660] - Positive Control: Mouse Gut Tissue 20 ug/ml](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NBP1-59660-img0002.jpg)
![Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of extracts from normal (control) and PRKAR2A knockout (KO) HeLa cells, using Protein Kinase A Regulatory Subunit II alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Protein-Kinase-A-Regulatory-Subunit-II-alpha-Antibody-Western-Blot-NBP3-03477-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of extracts from normal (control) and PRKAR2A knockout (KO) HeLa cells, using Protein Kinase A Regulatory Subunit II alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of L929 cells using [KO Validated] PRKAR2A Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Protein Kinase A Regulatory Subunit II alpha antibody](http://images.novusbio.com/fullsize/Protein-Kinase-A-Regulatory-Subunit-II-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03477-img0001.jpg)
![Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of extracts of various cell lines, using Protein Kinase A Regulatory Subunit II alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Protein-Kinase-A-Regulatory-Subunit-II-alpha-Antibody-Western-Blot-NBP3-03477-img0003.jpg)
![Immunohistochemistry: IP3R1 Antibody [NBP3-37937] - Perform microwave antigen retrievalwith 10 mM citrate buffer pH 6.0before commencing with IF stainingprotocol.Immunofluorescenceanalysis of paraffin-embedded RatBrain using IP3R Rabbit pAb(A21471) at dilution of 1:200 (40xlens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG(H+L) (AS007) at 1:500 dilution. Blue:DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37937_rabbit-ip3r1-pab-9122024159564.jpg "Immunohistochemistry: IP3R1 Antibody [NBP3-37937] - Perform microwave antigen retrievalwith 10 mM citrate buffer pH 6.0before commencing with IF stainingprotocol.Immunofluorescenceanalysis of paraffin-embedded RatBrain using IP3R Rabbit pAb(A21471) at dilution of 1:200 (40xlens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG(H+L) (AS007) at 1:500 dilution. Blue:DAPI for nuclear staining.")
![Immunoprecipitation: IP3R1 Antibody [NBP3-37937] - Immunoprecipitation of IP3R1 from 500 ug extracts of SH-SY5Y cells was performed using 2 ug of IP3R1 Rabbit pAb was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western Blot analysis of immunoprecipitates was conducted using IP3R1 Rabbit pAb at a dilution of 1:2500.](http://images.novusbio.com/fullsize/nbp3-37937_rabbit-ip3r1-pab-31120251704798.jpg)
![Western Blot: IP3R1 Antibody [NBP3-37937] - Western Blot analysis of various lysates using IP3R1 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): A-431.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-37937_rabbit-ip3r1-pab-311202517310.jpg)
![Western Blot: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Western blot analysis of extracts of various cell lines, using S5a/Angiocidin Rabbit mAb (NBP3-16240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-5G1X6-Western-Blot-NBP3-16240-img0002.jpg "Western Blot: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Western blot analysis of extracts of various cell lines, using S5a/Angiocidin Rabbit mAb (NBP3-16240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Immunofluorescence analysis of NIH-3T3 cells using S5a/Angiocidin Rabbit mAb (NBP3-16240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-5G1X6-Immunocytochemistry-Immunofluorescence-NBP3-16240-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Immunofluorescence analysis of NIH-3T3 cells using S5a/Angiocidin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16240_rabbit-s5a-angiocidin-mab-5g1x6-5220251255539.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Western-Blot-NBP3-16844-img0003.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunofluorescence analysis of C6 cells using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunocytochemistry-Immunofluorescence-NBP3-16844-img0002.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunohistochemistry of paraffin-embedded rat kidney using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunohistochemistry-Paraffin-NBP3-16844-img0001.jpg)
![Western Blot: ELAVL2 Antibody [NBP3-03894] - Analysis of extracts of various cell lines, using ELAVL2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/ELAVL2-Antibody-Western-Blot-NBP3-03894-img0004.jpg "Western Blot: ELAVL2 Antibody [NBP3-03894] - Analysis of extracts of various cell lines, using ELAVL2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP3-03894] - Paraffin-embedded mouse brain using ELAVL2 antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ELAVL2-Antibody-Immunohistochemistry-Paraffin-NBP3-03894-img0003.jpg)
![Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP3-03894] - Paraffin-embedded rat brain using ELAVL2 antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ELAVL2-Antibody-Immunohistochemistry-Paraffin-NBP3-03894-img0002.jpg)
![Western Blot: ZFPM1 Antibody [NBP3-05275] - Analysis of extracts of various cell lines, using ZFPM1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit](http://images.novusbio.com/fullsize/ZFPM1-Antibody-Western-Blot-NBP3-05275-img0001.jpg "Western Blot: ZFPM1 Antibody [NBP3-05275] - Analysis of extracts of various cell lines, using ZFPM1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit")
![Immunocytochemistry/Immunofluorescence: ZFPM1 Antibody [NBP3-05275] - Immunofluorescence analysis of C6 cells using ZFPM1 Rabbit pAb (NBP3-05275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ZFPM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05275-img0002.jpg)
![Western Blot: ZFPM1 Antibody - Azide and BSA Free [ZFPM1] - Western blot analysis of various lysates, using ZFPM1 Rabbit pAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-05275_rabbit-polyclonal-zfpm1-antibody-5220251638082.jpg)