| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37446-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47611-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59322-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52431-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61885-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89196-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27174SS | |||
![Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Western-Blot-NBP2-37446-img0003.jpg "Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Immunofluorescence analysis of HeLa cells using HAS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Immunocytochemistry-Immunofluorescence-NBP2-37446-img0004.jpg)
![Immunohistochemistry-Paraffin: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Analysis of ovarian cancer tissues using HAS2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Immunohistochemistry-NBP2-37446-img0005.jpg)
![Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mAb against human HAS2 recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Western-Blot-NBP2-37446-img0002.jpg)
![ELISA: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-ELISA-NBP2-37446-img0001.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of colon cancer tissues using FOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Immunohistochemistry-NBP2-37492-img0005.jpg "Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of colon cancer tissues using FOS mouse mAb with DAB staining.")
![Western Blot: c-Fos Antibody (2G2) [NBP2-37492] - Western blot analysis using FOS mouse mAb against HeLa (1), and HeLa (2) cell lysate.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Western-Blot-NBP2-37492-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of cerebellum tissues using FOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Immunohistochemistry-NBP2-37492-img0006.jpg)
![Flow Cytometry: c-Fos Antibody (2G2) [NBP2-37492] - Flow cytometric analysis of HeLa cells using FOS mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Flow-Cytometry-NBP2-37492-img0004.jpg)
![Western Blot: c-Fos Antibody (2G2) [NBP2-37492] - Western blot analysis using FOS mAb against human FOS (AA: 116-298) recombinant protein. (Expected MW is 45.8 kDa)](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Western-Blot-NBP2-37492-img0002.jpg)
![ELISA: c-Fos Antibody (2G2) [NBP2-37492] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-ELISA-NBP2-37492-img0001.jpg)
![Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NMDAR2B protein using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Western-Blot-NBP1-47611-img0008.jpg "Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NMDAR2B protein using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2B/NMDAR2B Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunocytochemistry-Immunofluorescence-NBP1-47611-img0010.jpg)
![Immunohistochemistry: NMDAR2B Antibody (S59) [NBP1-47611] - Immunohistochemistry analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunohistochemistry-NBP1-47611-img0009.jpg)
![Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - analysis of Human HEK-T lysates showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 . Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Western-Blot-NBP1-47611-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunocytochemistry-Immunofluorescence-NBP1-47611-img0005.jpg)
![Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322] - Western Blot analysis of Rat brain membrane lysate showing detection of CACNA1G Calcium Channel protein using Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Primary Antibody: Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody (NBP2-59322) at 1:1000.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Western-Blot-NBP2-59322-img0005.jpg "Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322] - Western Blot analysis of Rat brain membrane lysate showing detection of CACNA1G Calcium Channel protein using Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Primary Antibody: Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody (NBP2-59322) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) CACNA1G Antibody (D) Composite.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) CACNA1G Antibody. (D) Composite.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0007.jpg)
![Western Blot: Mouse Monoclonal CACNA1G Antibody (S178A-9) [NBP2-59322] - Western blot using lysates from human primary astrocytes or IGR39 melanoma cell line. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-59322_mouse-monoclonal-cacna1g-antibody-s178a-9-western-blot-1122023151422..png)
![Western Blot: NMDA R, NR2C Subunit Antibody [NB300-107]](http://images.novusbio.com/fullsize/NMDA-R--NR2C-Subunit-Antibody-Western-Blot-NB300-107-img0005.jpg "Western Blot: NMDA R, NR2C Subunit Antibody [NB300-107]")
![Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mouse mAb against A549 (1), 3T3-L1 (2), A431 (3) cell lysate.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-5E9E2-Western-Blot-NBP2-52431-img0008.jpg "Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mouse mAb against A549 (1), 3T3-L1 (2), A431 (3) cell lysate.")
![Immunohistochemistry-Paraffin: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of paraffin-embedded esophageal cancer tissues using CAV2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-5E9E2-Immunohistochemistry-Paraffin-NBP2-52431-img0006.jpg)
![Flow Cytometry: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of A549 cells using CAV2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-5E9E2-Flow-Cytometry-NBP2-52431-img0007.jpg)
![Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mAb against HEK293 (1) and CAV2 (AA: 1-86)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-5E9E2-Western-Blot-NBP2-52431-img0003.jpg)
![Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mAb against human CAV2 (AA: 1-86) recombinant protein. (Expected MW is 35.9 kDa)](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-5E9E2-Flow-Cytometry-NBP2-52431-img0002.jpg)
![Immunohistochemistry: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of paraffin-embedded esophageal cancer tissues using CAV2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-5E9E2-Immunohistochemistry-NBP2-52431-img0001.jpg)
![ELISA: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-5E9E2-ELISA-NBP2-52431-img0005.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mAb against HEK293 (1) and SOD2 (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Western-Blot-NBP2-61885-img0006.jpg "Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mAb against HEK293 (1) and SOD2 (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis of paraffin-embedded rectum cancer tissues using SOD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Immunohistochemistry-NBP2-61885-img0004.jpg)
![Flow Cytometry: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis of MCF-7 cells using SOD2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Flow-Cytometry-NBP2-61885-img0002.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mAb against human SOD2 (AA: 1-222) recombinant protein. (Expected MW is 50.7 kDa)](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Western-Blot-NBP2-61885-img0005.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mouse mAb against Hela (1), HepG2 (2), and SH-SY5Y (3) cell lysate.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Western-Blot-NBP2-61885-img0007.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis of paraffin-embedded breast cancer tissues using SOD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Immunohistochemistry-NBP2-61885-img0003.jpg)
![ELISA: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-ELISA-NBP2-61885-img0001.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-288202414233110.jpg "Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241435230.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33140, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241492693.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29202410484841.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33140, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-392024884679.jpg)
![Western Blot: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of MTCO2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysateLane 2: THP-1 cell lysate Lane 3: Hela cell lysate Lane 4: human lung tissue lysate Lane 5: human placenta tissue lysate](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Western-Blot-NBP2-67617-img0006.jpg "Western Blot: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of MTCO2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysateLane 2: THP-1 cell lysate Lane 3: Hela cell lysate Lane 4: human lung tissue lysate Lane 5: human placenta tissue lysate")
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunohistochemistry-Paraffin-NBP2-67617-img0005.jpg)
![Flow Cytometry: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Flow-Cytometry-NBP2-67617-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0003.jpg)
![Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-288202414191539.jpg "Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33093) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-29820241433367.jpg)
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33093, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-298202414304144.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-2920241114843.jpg)
![Flow Cytometry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Flow cytometric analysis of Hela cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33093, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-392024884612.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0002.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human testis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0001.jpg)
![Western Blot: HES5 Antibody [NBP2-27174] - analysis of HES5 in mouse liver tissue lysate in the 1) absence and 2) presence of immunizing peptide using HES5 antibody at 7 ug/ml. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HES5-Antibody-Western-Blot-NBP2-27174-img0002.jpg "Western Blot: HES5 Antibody [NBP2-27174] - analysis of HES5 in mouse liver tissue lysate in the 1) absence and 2) presence of immunizing peptide using HES5 antibody at 7 ug/ml. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: HES5 Antibody [NBP2-27174] - analysis of HES5 in formalin-fixed, paraffin-embedded human brain tissue using HES5 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/HES5-Antibody-Immunohistochemistry-Paraffin-NBP2-27174-img0001.jpg)