| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87103-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33555-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-89182SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33529-25ul | |||
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Western-Blot-NBP3-13660-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Immunocytochemistry-Immunofluorescence-NBP3-13660-img0002.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Immunohistochemistry-Paraffin-NBP3-13660-img0003.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Western-Blot-NBP3-13660-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-910202420131225.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Drosophila tissue extract (5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-9102024205691.jpg)
![A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-western-blot-261120251995423.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0016.jpg "Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0015.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0007.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human heart muscle shows moderate to strong nuclear positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining in human heart muscle and lymph node tissues.. Corresponding S100A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0012.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human lymph node shows no positivity in non - germinal center cells as expected.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0013.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human skin shows moderate to strong cytoplasmic positivity in melanocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0014.jpg)
![Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Western-Blot-NBP3-13575-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI/Quaking protein stained by QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Frozen-NBP3-13575-img0005.jpg)
![Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:3000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Western-Blot-NBP3-13575-img0001.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Paraffin-NBP3-13575-img0002.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Paraffin-NBP3-13575-img0003.jpg)
![QKI antibody [GT1589] detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: QKI protein stained by QKI antibody [GT1589] (NBP3-13575) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp3-13575_mouse-qki-quaking-mab-gt1589-immunocytochemistry-immunofluorescence-2611202518235322.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-2120249413328.jpg "Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Genetic Strategies Validation. </b>Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-2120249425474.jpg)
![Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-212024942544.jpg)
![Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-21202494142.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-2120249432896.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-212024938315.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-212024937492.jpg)
![Western Blot: MBP Antibody [NBP2-33555] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NBP2-33555-img0014.jpg "Western Blot: MBP Antibody [NBP2-33555] - Analysis in mouse cerebral cortex tissue.")
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse olfactory bulb shows immunoreactivity in processes in external plexiform layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0011.jpg)
![Western Blot: MBP Antibody [NBP2-33555] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NBP2-33555-img0013.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human cerebral cortex shows positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0005.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human hippocampus shows moderate immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0006.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human cerebellum shows moderate nuclear and cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0015.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse cerebellum shows immunoreactivity in granular cell layer and in white matter.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0007.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse cerebral cortex shows labeling of neuronal processes in motor cortex and in corpus callosum.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0008.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse dorsal tenia tecta shows positivity in neuronal processes.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0009.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse hippocampus shows positivity in neuronal processes in the CA1 layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249413358.jpg "Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-212024941419.jpg)
![Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Unboiled SH-SY5Y whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249413362.jpg)
![Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249383153.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249403855.jpg)
![Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in ice-cold MeOH for 5 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25443_rabbit-dopamine-d2r-drd2-mab-hl1478-azide-and-bsa-free-2120249362783.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NBP2-89182-img0003.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-89182-img0002.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of NPC1L1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NPC1L1 antibody generated moderate staining of hepatocyte cytoplasm.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NBP2-89182-img0001.jpg)
![Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Frozen-NBP3-13014-img0008.jpg "Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein in mouse retina by immunohistochemical analysis. Sample: Paraffin-embedded mouse retina. HCN1 antibody (NBP3-13014) diluted at 1:800. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0002.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HCN1 antibody (NBP3-13014) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0003.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HCN1 antibody (NBP3-13014) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0004.jpg)
![Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Frozen-NBP3-13014-img0005.jpg)
![HCN1 antibody detects HCN1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HCN1 stained by HCN1 antibody (NBP3-13014) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13014_rabbit-hcn1-pab-immunocytochemistry-immunofluorescence-261120251818921.jpg)
![GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-immunohistochemistry-paraffin-14112025183027.jpg "GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-immunohistochemistry-paraffin-1411202518415215.jpg)
![Unboiled human tissue extract was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-western-blot-141120251831213.jpg)
![<b>Orthogonal Strategies Validation. </b>GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-immunohistochemistry-paraffin-141120251844815.jpg)
![Unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-western-blot-1411202518341521.jpg)
![Boiled and unboiled rat tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48818_rabbit-gat-1-slc6a1-mab-hl3475-western-blot-141120251842522.jpg)
![GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25623_rabbit-relb-mab-hl2222-azide-and-bsa-free-immunoprecipitation-14112025185540.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-212024941471.jpg "Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249415939.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-212024942549.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249393563.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249415961.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249371590.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249355523.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Analysis in human parathyroid gland and liver tissues. Corresponding Parvalbumin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Analysis in human parathyroid gland and liver tissues. Corresponding Parvalbumin RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Parvalbumin Antibody [NBP2-33529] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NBP2-33529-img0015.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0014.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0008.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human cerebellum shows strong positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0011.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human cerebral cortex shows strong positivity in a small subset of neurons.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0012.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human kidney shows strong positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0013.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249415924.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-212024935136.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249432872.jpg)
![Immunohistochemistry-Frozen: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:300. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249393591.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249415945.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249342788.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249374965.jpg)