| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94524-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35166-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03748-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37513-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37281-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97747-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99063-50ul | |||
![Western Blot: Transglutaminase 5 Antibody - BSA Free [Transglutaminase 5] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Transglutaminase 5 using Transglutaminase 5 Rabbit pAb at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp2-94524_rabbit-polyclonal-transglutaminase-5-antibody-52202516345295.jpg "Western Blot: Transglutaminase 5 Antibody - BSA Free [Transglutaminase 5] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Transglutaminase 5 using Transglutaminase 5 Rabbit pAb at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded rat kidney using Transglutaminase 5 .](http://images.novusbio.com/fullsize/Transglutaminase-5-Antibody-Immunohistochemistry-Paraffin-NBP2-94524-img0003.jpg)
![Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded human colon using Transglutaminase 5 .](http://images.novusbio.com/fullsize/Transglutaminase-5-Antibody-Immunohistochemistry-Paraffin-NBP2-94524-img0001.jpg)
![Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded mouse heart using Transglutaminase 5 .](http://images.novusbio.com/fullsize/Transglutaminase-5-Antibody-Immunohistochemistry-Paraffin-NBP2-94524-img0002.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-35166] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using NFkB p105/p50 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202414123826.jpg "Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-35166] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using NFkB p105/p50 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: NFkB p105/p50 Antibody [NBP3-35166] - Western blot analysis of lysates from Raji cells, using NFkB p105/p50 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202414123838.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-35166] - Immunofluorescence analysis of MCF7 cells using NFkB p105/p50 Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202415175235.jpg)
![Immunoprecipitation: NFkB p105/p50 Antibody [NBP3-35166] - Immunoprecipitation analysis of 150 ug extracts of MCF-7 cells using 3 ug NFkB p105/p50 antibody. Western blot was performed from the immunoprecipitate using NFkB p105/p50 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202414201377.jpg)
![Western Blot:Tyrosine Hydroxylase Antibody (OTI1A4)[NBP3-44864]](http://images.novusbio.com/fullsize/nbp3-44864_mouse-tyrosine-hydroxylase-mab-oti1a4-western-blot-181202616191316.jpg "Western Blot:Tyrosine Hydroxylase Antibody (OTI1A4)[NBP3-44864]")
![Immunofluorescence:Tyrosine Hydroxylase Antibody (OTI1A4)[NBP3-44864]](http://images.novusbio.com/fullsize/nbp3-44864_mouse-tyrosine-hydroxylase-mab-oti1a4-immunofluorescence-181202616161910.jpg)
![Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI1A4)[NBP3-44864]](http://images.novusbio.com/fullsize/nbp3-44864_mouse-tyrosine-hydroxylase-mab-oti1a4-immunohistochemistry-181202616194115.jpg)
![Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI1A4)[NBP3-44864]](http://images.novusbio.com/fullsize/nbp3-44864_mouse-tyrosine-hydroxylase-mab-oti1a4-immunohistochemistry-18120261619138.jpg)
![Western Blot: Kv4.2 Antibody - BSA Free [NBP3-03748] - Western blot analysis of Mouse heart, using Kv4.2 antibody (A6203) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-03748_rabbit-polyclonal-kv4-2-antibody-5220251931375.jpg "Western Blot: Kv4.2 Antibody - BSA Free [NBP3-03748] - Western blot analysis of Mouse heart, using Kv4.2 antibody (A6203) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.")
![Western Blot: LRFN2 Antibody [NBP2-81731] - Western blot analysis of LRFN2 expression in mouse brain tissue lysate with LRFN2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/LRFN2-Antibody-Western-Blot-NBP2-81731-img0004.jpg "Western Blot: LRFN2 Antibody [NBP2-81731] - Western blot analysis of LRFN2 expression in mouse brain tissue lysate with LRFN2 antibody at 1 ug/ml.")
![Western Blot: LRFN2 Antibody [NBP2-81731] - Western blot analysis of LRFN2 expression in mouse brain tissue lysate with LRFN2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/LRFN2-Antibody-Western-Blot-NBP2-81731-img0001.jpg)
![Immunohistochemistry: LRFN2 Antibody - BSA Free [NBP2-81731] - Immunohistochemistry of LRFN2 in mouse brain tissue with LRFN2 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-81731_rabbit-polyclonal-lrfn2-antibody-2711202420285822.jpg)
![Immunocytochemistry/ Immunofluorescence: LRFN2 Antibody - BSA Free [NBP2-81731] - Immunofluorescence of LRFN2 in mouse brain tissue with LRFN2 antibody at 20 ugmL.](http://images.novusbio.com/fullsize/nbp2-81731_rabbit-polyclonal-lrfn2-antibody-2711202419353857.jpg)
![Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Western-Blot-NBP3-15454-img0002.jpg "Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Immunofluorescence analysis of Hep G2 cells using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Immunocytochemistry-Immunofluorescence-NBP3-15454-img0001.jpg)
![Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Western-Blot-NBP3-15454-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [Sonic Hedgehog/Shh] - Confocal imaging of paraffin-embedded Mouse kidney using Sonic Hedgehog/Shh Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15454_rabbit-sonic-hedgehog-shh-mab-8t4d3-522025171899.jpg)
![Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [Sonic Hedgehog/Shh] - Confocal imaging of Hep G2 cells using Sonic Hedgehog/Shh Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15454_rabbit-sonic-hedgehog-shh-mab-8t4d3-52202517192951.jpg)
![Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Western-Blot-NBP2-37513-img0003.jpg "Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.")
![Immunohistochemistry: Raf-1 Antibody (4G4) [NBP2-37513] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Immunohistochemistry-NBP2-37513-img0005.jpg)
![Flow Cytometry: Raf-1 Antibody (4G4) [NBP2-37513] - Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Flow-Cytometry-NBP2-37513-img0004.jpg)
![Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 48.6 kDa)](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Western-Blot-NBP2-37513-img0002.jpg)
![ELISA: Raf-1 Antibody (4G4) [NBP2-37513] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-ELISA-NBP2-37513-img0001.jpg)
![Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Western-Blot-NBP2-37281-img0003.jpg "Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Immunofluorescence analysis of Hela cells using GABBR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Immunocytochemistry-Immunofluorescence-NBP2-37281-img0004.jpg)
![Immunohistochemistry: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using GABBR2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Immunohistochemistry-NBP2-37281-img0005.jpg)
![Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against human GABBR2 (AA: 319-483) recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Western-Blot-NBP2-37281-img0002.jpg)
![ELISA: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-ELISA-NBP2-37281-img0001.jpg)
![Western Blot: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB9.](http://images.novusbio.com/fullsize/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Western-Blot-NBP2-72918-img0002.jpg "Western Blot: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB9.")
![Flow Cytometry: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - Analysis of Jurkat cells, using anti-NDUFB9 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72918-img0004.jpg)
![Western Blot: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NDUFB9 monoclonal antibody.](http://images.novusbio.com/fullsize/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Western-Blot-NBP2-72918-img0001.jpg)
![Flow Cytometry: NDUFB9 Antibody (OTI8B7) - Azide and BSA Free [NBP2-72918] - Analysis of Hela cells, using anti-NDUFB9 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NDUFB9-Antibody-OTI8B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72918-img0003.jpg)
![Western Blot: IP3R1 Antibody [NBP3-09066] - Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Western-Blot-NBP3-09066-img0001.jpg "Western Blot: IP3R1 Antibody [NBP3-09066] - Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.")
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP3-09066] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IP3 Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP3-09066-img0003.jpg)
![Flow Cytometry: IP3R1 Antibody [NBP3-09066] - Flow cytometric analysis of SH-SY-5Y cells with IP3 Receptor antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Flow-Cytometry-NBP3-09066-img0004.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP3-09066] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IP3 Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP3-09066-img0002.jpg)
![Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/ADNP-Antibody-Western-Blot-NBP2-97747-img0003.jpg "Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![Immunocytochemistry/Immunofluorescence: ADNP Antibody [NBP2-97747] - Immunofluorescence staining of ADNP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ADNP polyclonal antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97747-img0001.jpg)
![Immunoprecipitation: ADNP Antibody [NBP2-97747] - ADNP was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate. 4 ul anti-ADNP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ADNP rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa. Observed band size: 150 kDa.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunoprecipitation-NBP2-97747-img0004.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-99063-img0003.jpg "Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063] - Immunochemical staining of human Calbindin D-28K in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-99063-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063] - Immunofluorescence staining of Calbindin D-28K in 293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-99063 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202514331592.png)
![Immunoprecipitation: Calbindin D-28K Antibody [NBP2-99063] - Calbindin D-28K was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 4 ul NBP2-99063 and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: NBP2-99063, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202516335024.png)