| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59379-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97814-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48294 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44977 | |||
| Azide and BSA Free | NBP2-34817-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59628 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48410 | |||
| Azide and BSA Free | NBP3-11546 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47970-0.1mg | |||
| IHC-Prediluted | NBP2-48294 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44980 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85462-25ul | |||
![Western Blot: CLCN3 Antibody (S258-5) [NBP2-59379] - Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5 (NBP2-59379). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody (NBP2-59379) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.](http://images.novusbio.com/fullsize/CLCN3-Antibody-S258-5-Western-Blot-NBP2-59379-img0005.jpg "Western Blot: CLCN3 Antibody (S258-5) [NBP2-59379] - Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5 (NBP2-59379). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody (NBP2-59379) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.")
![Immunocytochemistry/Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Clcn3 Monoclonal Antibody, Clone S258-5 (NBP2-59379). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody (NBP2-59379) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Clcn3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/CLCN3-Antibody-S258-5-Immunocytochemistry-Immunofluorescence-NBP2-59379-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/CLCN3-Antibody-S258-5-Immunocytochemistry-Immunofluorescence-NBP2-59379-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Clcn3 Monoclonal Antibody, Clone S258-5 (NBP2-59379). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody (NBP2-59379) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Clcn3 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/CLCN3-Antibody-S258-5-Immunocytochemistry-Immunofluorescence-NBP2-59379-img0007.jpg)
![Western Blot: WWOX Antibody [NBP2-97814] - Anti-WWOX rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lane B: Mouse brain tissue lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-97814-img0003.jpg "Western Blot: WWOX Antibody [NBP2-97814] - Anti-WWOX rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lane B: Mouse brain tissue lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa")
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-97814] - Immunochemical staining of human WWOX in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-97814-img0004.jpg)
![Immunoprecipitation: WWOX Antibody [NBP2-97814] - WWOX was immunoprecipitated using: Lane A: 0.5 mg U251MG Whole Cell Lysate 4 ul anti-WWOX rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-WWOX rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunoprecipitation-NBP2-97814-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP2-97814] - Immunofluorescence staining of WWOX in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97814 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97814_rabbit-polyclonal-wwox-antibody-235202521451771.png)
![Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-278202415212420.jpg "Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32650) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-29820241420994.jpg)
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32650, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-298202414262675.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PS02-10) [NBP3-32650] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-2920241192341.jpg)
![Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-278202415212422.jpg "Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32649, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29820241420992.jpg)
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32649) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32649) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-298202414333659.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29202410234263.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47970-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).")
![Flow Cytometry: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - HEK293 cells using Neurofilament Monoclonal Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47970-img0002.jpg)
![Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Western-Blot-NBP2-32891-img0004.jpg "Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM589) [NBP2-32891] - Formalin-fixed paraffin-embedded human bladder carcinoma stained with p53 monoclonal antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Immunohistochemistry-Paraffin-NBP2-32891-img0003.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Knockout-Validated-NBP2-59626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Protein Array: p53 Antibody (TRP/816) [NBP2-59626] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Protein-Array-NBP2-59626-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Western-Blot-NBP2-59626-img0006.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) [NBP2-59626] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Mouse Monoclonal Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Immunohistochemistry-Paraffin-NBP2-59626-img0002.jpg)
![Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Knockout-Validated-NBP2-44982-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TRP/817).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Western-Blot-NBP2-44982-img0005.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/817) [NBP2-44982] - Human Colon Carcinoma stained with p53 Monoclonal Antibody (TRP/817)](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Immunohistochemistry-Paraffin-NBP2-44982-img0001.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) [NBP2-44893] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Immunohistochemistry-Paraffin-NBP2-44893-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) [NBP2-44893] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).")
![Flow Cytometry: NF-L Antibody (NFL/736) [NBP2-44893] - Flow Cytometric Analysis of HEK293 cells using NF-L Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Flow-Cytometry-NBP2-44893-img0002.jpg)
![Western Blot: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody (M01), clone 1A7.Lane 1: MDM2 transfected lysate(55.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Western-Blot-H00004193-M01-img0018.jpg "Western Blot: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody (M01), clone 1A7.Lane 1: MDM2 transfected lysate(55.2 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of monoclonal antibody to MDM2 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Immunohistochemistry-Paraffin-H00004193-M01-img0015.jpg)
![Western Blot: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of MDM2 over-expressed 293 cell line, cotransfected with MDM2 Validated Chimera RNAi ( Cat # H00004193-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MDM2 monoclonal antibody (M01), clone 1A7 (Cat # H00004193-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Western-Blot-H00004193-M01-img0016.jpg)
![Proximity Ligation Assay: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of protein-protein interactions between TP53 and MDM2. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-MDM2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Proximity-Ligation-Assay-H00004193-M01-img0013.jpg)
![ELISA: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Detection limit for recombinant GST tagged MDM2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-ELISA-H00004193-M01-img0019.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) - Azide and BSA Free [NBP2-34563] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-+-DO-7-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34563-img0001.jpg "Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) - Azide and BSA Free [NBP2-34563] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)")
![Immunocytochemistry/Immunofluorescence: Raf-1 Antibody [NBP1-85462] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody stainig is shown in green.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85462-img0006.jpg "Immunocytochemistry/Immunofluorescence: Raf-1 Antibody [NBP1-85462] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody stainig is shown in green.")
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human testis shows weak positivity in nuclear speckles in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0011.jpg)
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human cerebellum shows moderate positivity in nuclear speckles in Purkinje cells.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0008.jpg)
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human cervix shows moderate positivity in nuclear speckles in squamous epithelial cells.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0009.jpg)
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human rectum shows moderate positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0010.jpg)