| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84265-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81326-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77309-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94845-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61666-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61660-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26502-50ul | |||
![Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)](http://images.novusbio.com/fullsize/SLC12A3-[p-Thr53]-Antibody-Western-Blot-NBP2-60775-img0001.jpg "Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)")
![Western Blot: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Western blot analysis of Galactosylceramidase/GALC on different lysates with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Rat brain tissue lysate Lane 10: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 77 kDa Observed band size: 60 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32361) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32361_rabbit-galactosylceramidase-galc-mab-psh03-45-278202416285223.jpg "Western Blot: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Western blot analysis of Galactosylceramidase/GALC on different lysates with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Rat brain tissue lysate Lane 10: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 77 kDa Observed band size: 60 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32361) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Galactosylceramidase/GALC Antibody (PSH03-45) [NBP3-32361] - Immunocytochemistry analysis of A549 cells labeling Galactosylceramidase/GALC with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Galactosylceramidase/GALC antibody (NBP3-32361) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32361_rabbit-galactosylceramidase-galc-mab-psh03-45-29202410234291.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)
![Immunohistochemistry-Paraffin: WFS1 Antibody [NBP1-84265] - Staining of human Pancreas shows strong granular cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/WFS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84265-img0007.jpg "Immunohistochemistry-Paraffin: WFS1 Antibody [NBP1-84265] - Staining of human Pancreas shows strong granular cytoplasmic positivity in islets of Langerhans.")
![Immunohistochemistry-Paraffin: WFS1 Antibody [NBP1-84265] - Staining of human Heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/WFS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84265-img0005.jpg)
![Immunohistochemistry-Paraffin: WFS1 Antibody [NBP1-84265] - Staining of human Lung shows strong granular cytoplasmic positivity in pneumocytes.](http://images.novusbio.com/fullsize/WFS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84265-img0006.jpg)
![Immunohistochemistry-Paraffin: WFS1 Antibody [NBP1-84265] - Staining of human Testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and leydig cells.](http://images.novusbio.com/fullsize/WFS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84265-img0008.jpg)
![Immunohistochemistry-Paraffin: MAN2B1 Antibody [NBP1-81326] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MAN2B1-Antibody-Immunohistochemistry-Paraffin-NBP1-81326-img0004.jpg "Immunohistochemistry-Paraffin: MAN2B1 Antibody [NBP1-81326] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.")
![Western Blot: APP Antibody (J4H9) [NBP1-04263] - The lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse brain tissue lysate.](http://images.novusbio.com/fullsize/APP-Antibody-J4H9-Western-Blot-NBP1-04263-img0003.jpg "Western Blot: APP Antibody (J4H9) [NBP1-04263] - The lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse brain tissue lysate.")
![Immunocytochemistry/Immunofluorescence: APP Antibody (J4H9) [NBP1-04263] - Analysis of APP/Protease Nexin II in U87MG cells. The cell was stained using a dilution of (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/APP-Antibody-J4H9-Immunocytochemistry-Immunofluorescence-NBP1-04263-img0002.jpg)
![Flow Cytometry: APP Antibody (J4H9) [NBP1-04263] - Flow analysis of APP/Protease Nexin II in 293T cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/APP-Antibody-J4H9-Flow-Cytometry-NBP1-04263-img0001.jpg)
![Western Blot: BACE-2 Antibody [NBP1-77309] - Human (A) and mouse (B) heart tissue lysates with BACE2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/BACE-2-Antibody-Western-Blot-NBP1-77309-img0002.jpg "Western Blot: BACE-2 Antibody [NBP1-77309] - Human (A) and mouse (B) heart tissue lysates with BACE2 antibody at 1 ug/ml.")
![Immunohistochemistry: BACE-2 Antibody - BSA Free [NBP1-77309] - Immunohistochemistry of BACE-2 in mouse heart with BACE-2 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77309_rabbit-polyclonal-bace-2-antibody-271120242022943.jpg)
![Western Blot: WFS1 Antibody - BSA Free [NBP2-94845] - Western blot analysis of various lysates, using WFS1 antibody (A1705) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp2-94845_rabbit-polyclonal-wfs1-antibody-5220251931379.jpg "Western Blot: WFS1 Antibody - BSA Free [NBP2-94845] - Western blot analysis of various lysates, using WFS1 antibody (A1705) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.")
![Immunocytochemistry/ Immunofluorescence: WFS1 Antibody - BSA Free [NBP2-94845] - Immunofluorescence analysis of HeLa cells using WFS1 Rabbit pAb (A1705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94845_rabbit-polyclonal-wfs1-antibody-5220251931386.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibody (4E2D1) [NBP2-61666] - Analysis using CHRNA10 mAb against human CHRNA10 (AA: extra 25-237) recombinant protein. (Expected MW is 50 kDa)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-10-CHRNA10-Antibody-4E2D1-Western-Blot-NBP2-61666-img0003.jpg "Western Blot: Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibody (4E2D1) [NBP2-61666] - Analysis using CHRNA10 mAb against human CHRNA10 (AA: extra 25-237) recombinant protein. (Expected MW is 50 kDa)")
![Flow Cytometry: Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibody (4E2D1) [NBP2-61666] - Analysis of Hela cells using CHRNA10 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-10-CHRNA10-Antibody-4E2D1-Flow-Cytometry-NBP2-61666-img0002.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibody (4E2D1) [NBP2-61666] - Analysis using CHRNA10 mAb against HEK293 (1) and CHRNA10 (AA: extra 25-237)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-10-CHRNA10-Antibody-4E2D1-Western-Blot-NBP2-61666-img0004.jpg)
![ELISA: Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibody (4E2D1) [NBP2-61666] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-10-CHRNA10-Antibody-4E2D1-ELISA-NBP2-61666-img0001.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibody (6C10E8) [NBP2-61660] - Analysis using CHRNA10 mAb against human CHRNA10 (AA: extra 25-237) recombinant protein. (Expected MW is 50 kDa)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-10-CHRNA10-Antibody-6C10E8-Western-Blot-NBP2-61660-img0003.jpg "Western Blot: Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibody (6C10E8) [NBP2-61660] - Analysis using CHRNA10 mAb against human CHRNA10 (AA: extra 25-237) recombinant protein. (Expected MW is 50 kDa)")
![Flow Cytometry: Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibody (6C10E8) [NBP2-61660] - Analysis of Hela cells using CHRNA10 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-10-CHRNA10-Antibody-6C10E8-Flow-Cytometry-NBP2-61660-img0002.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibody (6C10E8) [NBP2-61660] - Analysis using CHRNA10 mAb against HEK293 (1) and CHRNA10 (AA: extra 25-237)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-10-CHRNA10-Antibody-6C10E8-Western-Blot-NBP2-61660-img0004.jpg)
![ELISA: Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibody (6C10E8) [NBP2-61660] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-10-CHRNA10-Antibody-6C10E8-ELISA-NBP2-61660-img0001.jpg)
![Western Blot: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33074) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33074) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33074_mouse-uba3-mab-1c10-5-5-r-28820241419157.jpg "Western Blot: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33074) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33074) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-UBA3 antibody (NBP3-33074) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33074) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33074_mouse-uba3-mab-1c10-5-5-r-298202414262662.jpg)
![Western Blot: APP Antibody (6F10) [NBP3-26502] - Positive Western Blot detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: APP Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa Observed band size: 100 kDa](http://images.novusbio.com/fullsize/nbp3-26502_rabbit-app-mab-6f10-282202411413611.jpg "Western Blot: APP Antibody (6F10) [NBP3-26502] - Positive Western Blot detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: APP Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa Observed band size: 100 kDa")
![Immunocytochemistry/Immunofluorescence: APP Antibody (6F10) [NBP3-26502] - Staining of Hela Cells with APP Antibody (6F10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26502_rabbit-app-mab-6f10-28220241312218.jpg)