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![Immunohistochemistry-Paraffin: GPR50 Antibody [NLS751] - Analysis of anti-GPR50 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/GPR50-Antibody-Immunohistochemistry-Paraffin-NLS751-img0003.jpg "Immunohistochemistry-Paraffin: GPR50 Antibody [NLS751] - Analysis of anti-GPR50 antibody with human brain, glioblastoma.")
![Immunohistochemistry-Paraffin: GPR50 Antibody [NLS751] - Analysis of anti-GPR50 antibody with human brain, hypothalamus at 10 ug/ml.](http://images.novusbio.com/fullsize/GPR50-Antibody-Immunohistochemistry-Paraffin-NLS751-img0002.jpg)
![Western Blot: TR4/NR2C2 Antibody (JE45-03) [NBP3-33040] - Western blot analysis of TR4/NR2C2 on different lysates with Rabbit anti-TR4/NR2C2 antibody (NBP3-33040) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Jurkat cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33040) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33040_rabbit-tr4-nr2c2-mab-je45-03-288202412493418.jpg "Western Blot: TR4/NR2C2 Antibody (JE45-03) [NBP3-33040] - Western blot analysis of TR4/NR2C2 on different lysates with Rabbit anti-TR4/NR2C2 antibody (NBP3-33040) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Jurkat cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33040) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TR4/NR2C2 Antibody (JE45-03) [NBP3-33040] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TR4/NR2C2 antibody (NBP3-33040) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33040) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33040_rabbit-tr4-nr2c2-mab-je45-03-298202414304173.jpg)
![Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP2-82290] - Host: Mouse. Target Name: CHRNB2. Sample Tissue: Mouse Testis. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-Receptor-beta-2-Antibody-Western-Blot-NBP2-82290-img0002.jpg "Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP2-82290] - Host: Mouse. Target Name: CHRNB2. Sample Tissue: Mouse Testis. Antibody Dilution: 1ug/ml")
![Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP2-82290] - WB Suggested Anti-CHRNB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-Receptor-beta-2-Antibody-Western-Blot-NBP2-82290-img0001.jpg)
![Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP2-82290] - Host: Rabbit. Target Name: CHRNB2. Sample Tissue: Human HT1080 Whole Cell. Antibody Dilution: 3ug/ml](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-Receptor-beta-2-Antibody-Western-Blot-NBP2-82290-img0003.jpg)
![Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP2-82291] - WB Suggested Anti-CHRNB2 Antibody. Titration: 1.25 ug/ml. Positive Control: HepG2 Whole Cell](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-Receptor-beta-2-Antibody-Western-Blot-NBP2-82291-img0002.jpg "Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP2-82291] - WB Suggested Anti-CHRNB2 Antibody. Titration: 1.25 ug/ml. Positive Control: HepG2 Whole Cell")
![Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP2-82291] - Host: Mouse. Target Name: CHRNB2. Sample Tissue: Mouse Testis. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-Receptor-beta-2-Antibody-Western-Blot-NBP2-82291-img0001.jpg)
![Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP2-82291] - Host: Rabbit. Target Name: CHRNB2. Sample Tissue: Human HT1080 Whole Cell. Antibody Dilution: 3ug/ml](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-Receptor-beta-2-Antibody-Western-Blot-NBP2-82291-img0003.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (MEM-188) [NBP3-09126] - Analysis (surface staining) of human peripheral blood lymphocytes with anti-CD56 (MEM-188) purified; GAM-APC.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-MEM-188-Flow-Cytometry-NBP3-09126-img0001.jpg "Flow Cytometry: NCAM-1/CD56 Antibody (MEM-188) [NBP3-09126] - Analysis (surface staining) of human peripheral blood lymphocytes with anti-CD56 (MEM-188) purified; GAM-APC.")
![Immunocytochemistry/Immunofluorescence: CGRP Antibody (4901) [NBP2-88945] - Immunostaining of CGRP (red) in cryopreserved DRG neurons grown on coverslips. 40xmag. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5. Cells were obtained photo was provided courtesy of QBMCellScience.](http://images.novusbio.com/fullsize/CGRP-Antibody-4901-Immunocytochemistry-Immunofluorescence-NBP2-88945-img0001.jpg "Immunocytochemistry/Immunofluorescence: CGRP Antibody (4901) [NBP2-88945] - Immunostaining of CGRP (red) in cryopreserved DRG neurons grown on coverslips. 40xmag. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5. Cells were obtained photo was provided courtesy of QBMCellScience.")
![Western Blot: SIN3A Antibody [NB100-2253] - Detection of Human Sin3A by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody NB100-2253 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Western-Blot-NB100-2253-img0002.jpg "Western Blot: SIN3A Antibody [NB100-2253] - Detection of Human Sin3A by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody NB100-2253 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation: SIN3A Antibody [NB100-2253] - Detection of Human and Mouse Sin3A on HeLa whole cell lysate using NB100-2253. Sin3A was also immunoprecipitated using rabbit anti-Sin3A antibodies NB100-2252, NB100-2254 and another Sin3A antibody.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunoprecipitation-NB100-2253-img0001.jpg)
![Western Blot: USP7 Antibody (6512) [NBP2-43619] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 5 % SDS-PAGE USP7 antibody [6512] dilution: 1:1000](http://images.novusbio.com/fullsize/USP7-Antibody-6512-Western-Blot-NBP2-43619-img0001.jpg "Western Blot: USP7 Antibody (6512) [NBP2-43619] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 5 % SDS-PAGE USP7 antibody [6512] dilution: 1:1000")
![USP7 antibody [GT6512] detects USP7 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: USP7 protein stained by USP7 antibody [GT6512] (NBP2-43619) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43619_mouse-monoclonal-usp7-antibody-6512-immunocytochemistry-immunofluorescence-2611202518342614.jpg)
![Western Blot: Amisyn Antibody [NBP2-15376] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Amisyn-Antibody-Western-Blot-NBP2-15376-img0002.jpg "Western Blot: Amisyn Antibody [NBP2-15376] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Amisyn Antibody [NBP2-15376] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Amisyn-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15376-img0001.jpg)
![Western Blot: Insulin Receptor beta Antibody [NB120-10991] - Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor, b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor, b subunit (~97kDa)](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Western-Blot-NB120-10991-img0005.jpg "Western Blot: Insulin Receptor beta Antibody [NB120-10991] - Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor, b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor, b subunit (~97kDa)")
![Immunoprecipitation: Insulin Receptor beta Antibody [NB120-10991] - Immunoprecipitation 10 ug of this antibody immunoprecipitated a-Insulin Receptor, Beta subunit from 500 micrograms of Hek293 cell lysate using protein A agarose beads. Arrow indicates a-Insulin Receptor, Beta subunit (97 kDa).](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Immunoprecipitation-NB120-10991-img0004.jpg)
![Western Blot: MCT8/SLC16A2 Antibody [NBP2-32109] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-MCT8 antibody NBP2-32109 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCT8-SLC16A2-Antibody-Western-Blot-NBP2-32109-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCT8/SLC16A2 Antibody [NBP2-32109] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-MCT8 antibody NBP2-32109 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: MCT8/SLC16A2 Antibody [NBP2-32109] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells. Antibodies: Affinity purified rabbit anti-MCT8 antibodyNBP2-32109 used for IP at 6 ug per reaction. MCT8 was also immunoprecipitated by rabbit anti-MCT8 antibody BL16310. For blotting immunoprecipitated MCT8,NBP2-32109 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCT8-SLC16A2-Antibody-Western-Blot-NBP2-32109-img0002.jpg)
![Western Blot: Staufen Antibody [NBP2-22308] - Whole cell lysate (50 ug) from 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. NBP2-22308 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP2-22308-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Staufen Antibody [NBP2-22308] - Whole cell lysate (50 ug) from 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. NBP2-22308 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Staufen Antibody [NBP2-22308] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22308 used for IP at 6 ug/mg lysate. Staufen1 was also immunoprecipitated by rabbit anti-Staufen1 antibody. For blotting immunoprecipitated Staufen1, NBP2-22308 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunoprecipitation-NBP2-22308-img0002.jpg)