| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93530-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35166-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37513-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12913-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59326-25ug | |||
![Western Blot: VDAC3 Antibody [NBP1-80070] - Human Fetal Muscle, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Western-Blot-NBP1-80070-img0006.jpg "Western Blot: VDAC3 Antibody [NBP1-80070] - Human Fetal Muscle, Antibody Dilution: 1.0 ug/ml.")
![Immunohistochemistry: VDAC3 Antibody [NBP1-80070] - Human Lung cell Cellular data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Immunohistochemistry-NBP1-80070-img0003.jpg)
![Western Blot: VDAC3 Antibody [NBP1-80070] - Human Heart lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Western-Blot-NBP1-80070-img0002.jpg)
![Western Blot: VDAC3 Antibody [NBP1-80070] - Analysis of 721_B cell lysate. Antibody Dilution: 1.0 ug/ml VDAC3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Western-Blot-NBP1-80070-img0004.jpg)
![Western Blot: VDAC3 Antibody [NBP1-80070] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Western-Blot-NBP1-80070-img0005.jpg)
![Western Blot: MYRIP Antibody [NB100-1278] - Staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.3ug/ml)](http://images.novusbio.com/fullsize/MYRIP-Antibody-Western-Blot-NB100-1278-img0001.jpg "Western Blot: MYRIP Antibody [NB100-1278] - Staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.3ug/ml)")
![Immunohistochemistry-Paraffin: MYRIP Antibody [NB100-1278] - staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MYRIP-Antibody-Immunohistochemistry-Paraffin-NB100-1278-img0004.jpg)
![Immunohistochemistry-Paraffin: MYRIP Antibody [NB100-1278] - staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MYRIP-Antibody-Immunohistochemistry-Paraffin-NB100-1278-img0002.jpg)
![Immunohistochemistry-Paraffin: MYRIP Antibody [NB100-1278] - staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MYRIP-Antibody-Immunohistochemistry-Paraffin-NB100-1278-img0003.jpg)
![Western Blot: CEND1 Antibody (1D3Z1) [NBP3-16614] - Western blot analysis of extracts of various cell lines, using CEND1 Rabbit mAb (NBP3-16614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CEND1-Antibody-1D3Z1-Western-Blot-NBP3-16614-img0003.jpg "Western Blot: CEND1 Antibody (1D3Z1) [NBP3-16614] - Western blot analysis of extracts of various cell lines, using CEND1 Rabbit mAb (NBP3-16614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Immunofluorescence analysis of mouse brain cells using CEND1 Rabbit mAb (NBP3-16614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CEND1-Antibody-1D3Z1-Immunocytochemistry-Immunofluorescence-NBP3-16614-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Immunofluorescence analysis of rat brain cells using CEND1 Rabbit mAb (NBP3-16614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CEND1-Antibody-1D3Z1-Immunocytochemistry-Immunofluorescence-NBP3-16614-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Confocal imaging of paraffin-embedded Rat brain tissue using CEND1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16614_rabbit-cend1-mab-1d3z1-5220251242528.jpg)
![Immunocytochemistry/ Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Confocal imaging of paraffin-embedded Mouse brain tissue using CEND1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16614_rabbit-cend1-mab-1d3z1-52202511383719.jpg)
![Western Blot: Phospholipid Scramblase 1/PLSCR1 Antibody (5U6G1) [NBP3-16183] - Western blot analysis of extracts of HT-29 cells, using Phospholipid Phospholipid Scramblase 1/PLSCR1 (PLSCR1) Rabbit mAb (NBP3-16183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Phospholipid-Scramblase-1-PLSCR1-Antibody-5U6G1-Western-Blot-NBP3-16183-img0004.jpg "Western Blot: Phospholipid Scramblase 1/PLSCR1 Antibody (5U6G1) [NBP3-16183] - Western blot analysis of extracts of HT-29 cells, using Phospholipid Phospholipid Scramblase 1/PLSCR1 (PLSCR1) Rabbit mAb (NBP3-16183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: Phospholipid Scramblase 1/PLSCR1 Antibody (5U6G1) [NBP3-16183] - Western blot analysis of extracts of Mouse lung, using Phospholipid Phospholipid Scramblase 1/PLSCR1 (PLSCR1) Rabbit mAb (NBP3-16183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Phospholipid-Scramblase-1-PLSCR1-Antibody-5U6G1-Western-Blot-NBP3-16183-img0005.jpg)
![Immunohistochemistry-Paraffin: Phospholipid Scramblase 1/PLSCR1 Antibody (5U6G1) [NBP3-16183] - Immunohistochemistry of paraffin-embedded human breast cancer using Phospholipid Phospholipid Scramblase 1/PLSCR1 (PLSCR1) Rabbit mAb (NBP3-16183) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Phospholipid-Scramblase-1-PLSCR1-Antibody-5U6G1-Immunohistochemistry-Paraffin-NBP3-16183-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Phospholipid Scramblase 1/PLSCR1 Antibody (5U6G1) [NBP3-16183] - Immunofluorescence analysis of C6 cells using Phospholipid Phospholipid Scramblase 1/PLSCR1 (PLSCR1) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16183_rabbit-phospholipid-scramblase-1-plscr1-mab-5u6g1-522025125479.jpg)
![Western Blot: DISC1 Antibody (4U5N1) [NBP3-16462] - Western blot analysis of extracts of various cell lines, using DISC1 Rabbit mAb (NBP3-16462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/DISC1-Antibody-4U5N1-Western-Blot-NBP3-16462-img0004.jpg "Western Blot: DISC1 Antibody (4U5N1) [NBP3-16462] - Western blot analysis of extracts of various cell lines, using DISC1 Rabbit mAb (NBP3-16462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody (4U5N1) [NBP3-16462] - Immunofluorescence analysis of U-2 OS cells using DISC1 Rabbit mAb (NBP3-16462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DISC1-Antibody-4U5N1-Immunocytochemistry-Immunofluorescence-NBP3-16462-img0003.jpg)
![Western Blot: DISC1 Antibody (4U5N1) [NBP3-16462] - Western blot analysis of extracts of various cell lines, using DISC1 Rabbit mAb (NBP3-16462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/DISC1-Antibody-4U5N1-Western-Blot-NBP3-16462-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody (4U5N1) [NBP3-16462] - Immunofluorescence analysis of C6 cells using DISC1 Rabbit mAb (NBP3-16462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DISC1-Antibody-4U5N1-Immunocytochemistry-Immunofluorescence-NBP3-16462-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody (4U5N1) [NBP3-16462] - Immunofluorescence analysis of NIH-3T3 cells using DISC1 Rabbit mAb (NBP3-16462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DISC1-Antibody-4U5N1-Immunocytochemistry-Immunofluorescence-NBP3-16462-img0002.jpg)
![Western Blot: Nicalin Antibody [NBP2-81860] - Western blot analysis of Nicalin in mouse heart tissue lysate with Nicalin antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Nicalin-Antibody-Western-Blot-NBP2-81860-img0001.jpg "Western Blot: Nicalin Antibody [NBP2-81860] - Western blot analysis of Nicalin in mouse heart tissue lysate with Nicalin antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: Nicalin Antibody [NBP2-81860] - Immunofluorescence of Nicalin in human heart tissue with Nicalin antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Nicalin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81860-img0003.jpg)
![Immunohistochemistry: Nicalin Antibody [NBP2-81860] - Immunohistochemistry of Nicalin in human heart tissue with Nicalin antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/Nicalin-Antibody-Immunohistochemistry-NBP2-81860-img0002.jpg)
![Western Blot: Nicalin Antibody - BSA Free [NBP2-81860] - Western blot analysis of Nicalin in mouse heart tissue lysate with Nicalin antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp2-81860_rabbit-polyclonal-nicalin-antibody-2711202419241381.gif)
![Western Blot: MT-ND4 Antibody [NBP2-93530] - Analysis of extracts of various cell lines, using MT-ND4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Western-Blot-NBP2-93530-img0005.jpg "Western Blot: MT-ND4 Antibody [NBP2-93530] - Analysis of extracts of various cell lines, using MT-ND4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: MT-ND4 Antibody [NBP2-93530] - Analysis of L929 cells using MT-ND4 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93530-img0001.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-93530] -Analysis of rat kidney using MT-ND4 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-93530_rabbit-polyclonal-mt-nd4-antibody-immunohistochemistry-paraffin-247202491744..jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-93530] -Analysis of Rat ovary using MT-ND4 Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp2-93530_rabbit-polyclonal-mt-nd4-antibody-immunohistochemistry-paraffin-247202491923..jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-35166] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using NFkB p105/p50 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202414123826.jpg "Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-35166] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using NFkB p105/p50 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: NFkB p105/p50 Antibody [NBP3-35166] - Western blot analysis of lysates from Raji cells, using NFkB p105/p50 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202414123838.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-35166] - Immunofluorescence analysis of MCF7 cells using NFkB p105/p50 Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202415175235.jpg)
![Immunoprecipitation: NFkB p105/p50 Antibody [NBP3-35166] - Immunoprecipitation analysis of 150 ug extracts of MCF-7 cells using 3 ug NFkB p105/p50 antibody. Western blot was performed from the immunoprecipitate using NFkB p105/p50 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202414201377.jpg)
![Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Western-Blot-NBP2-37513-img0003.jpg "Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.")
![Immunohistochemistry: Raf-1 Antibody (4G4) [NBP2-37513] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Immunohistochemistry-NBP2-37513-img0005.jpg)
![Flow Cytometry: Raf-1 Antibody (4G4) [NBP2-37513] - Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Flow-Cytometry-NBP2-37513-img0004.jpg)
![Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 48.6 kDa)](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Western-Blot-NBP2-37513-img0002.jpg)
![ELISA: Raf-1 Antibody (4G4) [NBP2-37513] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-ELISA-NBP2-37513-img0001.jpg)
![Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Western-Blot-NB300-236-img0001.jpg "Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![Immunohistochemistry: Synaptotagmin-1 [p Ser309] Antibody [NB300-236] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Immunohistochemistry-NB300-236-img0002.jpg)
![Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Western-Blot-NBP2-12913-img0003.jpg "Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK2 (pan) Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Immunocytochemistry-Immunofluorescence-NBP2-12913-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK2 (pan) Antibody. (D) Composite.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Immunocytochemistry-Immunofluorescence-NBP2-12913-img0005.jpg)
![Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Western-Blot-NBP2-59326-img0007.jpg "Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody (NBP2-59326) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Immunocytochemistry-Immunofluorescence-NBP2-59326-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Analysis using Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody, Clone S398A-34. (Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Immunocytochemistry-Immunofluorescence-NBP2-59326-img0004.jpg)