| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58256-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58100-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92462-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89210-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86239-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-97515-0.025mg | |||
![Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58256] - Staining of human cerebral cortex, eye, liver and lymph node using Anti-Cytokeratin 12 antibody NBP2-58256 (A) shows similar protein distribution across tissues to independent antibody NBP2-58100 (B).](http://images.novusbio.com/fullsize/Cytokeratin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-58256-img0008.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58256] - Staining of human cerebral cortex, eye, liver and lymph node using Anti-Cytokeratin 12 antibody NBP2-58256 (A) shows similar protein distribution across tissues to independent antibody NBP2-58100 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58256] - Staining of human eye shows strong cytoplasmic positivity in the corneal epithelial cells.](http://images.novusbio.com/fullsize/Cytokeratin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-58256-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58256] - Staining of human liver.](http://images.novusbio.com/fullsize/Cytokeratin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-58256-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58256] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Cytokeratin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-58256-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58256] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Cytokeratin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-58256-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58100] - Staining of human eye.](http://images.novusbio.com/fullsize/Cytokeratin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-58100-img0008.jpg "Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58100] - Staining of human eye.")
![Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58100] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Cytokeratin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-58100-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58100] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Cytokeratin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-58100-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58100] - Staining of human liver.](http://images.novusbio.com/fullsize/Cytokeratin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-58100-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody [NBP2-58100] - Staining of human cerebral cortex, eye, liver and lymph node using Anti-Cytokeratin 12 antibody NBP2-58100 (A) shows similar protein distribution across tissues to independent antibody NBP2-58256 (B).](http://images.novusbio.com/fullsize/Cytokeratin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-58100-img0007.jpg)
![Immunohistochemistry-Paraffin: SV2B Antibody [NBP1-92462] - Staining of human cerebral cortex shows distinct positivity in neuropil.](http://images.novusbio.com/fullsize/SV2B-Antibody-Immunohistochemistry-Paraffin-NBP1-92462-img0001.jpg "Immunohistochemistry-Paraffin: SV2B Antibody [NBP1-92462] - Staining of human cerebral cortex shows distinct positivity in neuropil.")
![Immunohistochemistry: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Immunohistochemistry analysis of paraffin-embedded Rat cornea using Cytokeratin 12(KRT12) (KRT12) Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33197_rabbit-cytokeratin-12-mab-2m1k10-91220241418136.jpg "Immunohistochemistry: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Immunohistochemistry analysis of paraffin-embedded Rat cornea using Cytokeratin 12(KRT12) (KRT12) Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.")
![Immunohistochemistry: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Immunohistochemistry analysis of paraffin-embedded Mouse cornea using Cytokeratin 12(KRT12) (KRT12) Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33197_rabbit-cytokeratin-12-mab-2m1k10-91220241581233.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Confocal imaging of paraffin-embedded Rat eyeball tissue using Cytokeratin 12 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-33197_rabbit-cytokeratin-12-mab-2m1k10-5220251255535.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Confocal imaging of paraffin-embedded Mouse eyeball tissue using Cytokeratin 12 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-33197_rabbit-cytokeratin-12-mab-2m1k10-5220251215484.jpg)
![Western Blot: Pax6 Antibody (GT9412) [NBP3-13636] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/Pax6-Antibody-GT9412-Western-Blot-NBP3-13636-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Pax6 Antibody (GT9412) [NBP3-13636] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pax6-Antibody-GT9412-Immunohistochemistry-Paraffin-NBP3-13636-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunofluorescent analysis.Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-13636_mouse-pax6-mab-gt9412-91020242011450.jpg)
![Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB100-61654-img0002.jpg "Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Pax6 Antibody [NB100-61654] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Flow-Cytometry-NB100-61654-img0003.jpg)
![Western Blot: Pax6 Antibody [NB100-61654] - (0.3ug/ml) staining of U251 (A), (0.03ug/ml) nuclear K562 (B) and (1ug/ml) nuclear HeLa (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB100-61654-img0001.jpg)
![Western Blot: DDR2 Antibody (3B11E4) [NBP1-28883] - Analysis using DDR2 mouse mAb against truncated DDR2 recombinant protein.](http://images.novusbio.com/fullsize/DDR2-Antibody-3B11E4-Western-Blot-NBP1-28883-img0009.jpg "Western Blot: DDR2 Antibody (3B11E4) [NBP1-28883] - Analysis using DDR2 mouse mAb against truncated DDR2 recombinant protein.")
![Immunocytochemistry/Immunofluorescence: DDR2 Antibody (3B11E4) [NBP1-28883] - Analysis of A549 cells using DDR2 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/DDR2-Antibody-3B11E4-Immunocytochemistry-Immunofluorescence-NBP1-28883-img0006.jpg)
![Immunohistochemistry-Paraffin: DDR2 Antibody (3B11E4) [NBP1-28883] - Analysis of human skeletal musle tissues using DDR2 mouse mAb.](http://images.novusbio.com/fullsize/DDR2-Antibody-3B11E4-Immunohistochemistry-Paraffin-NBP1-28883-img0008.jpg)
![Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Western-Blot-NBP2-89210-img0001.jpg "Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa")
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0003.jpg)
![Immunohistochemistry-Paraffin: SV2C Antibody [NBP1-86239] - Staining of human prostate shows moderate cytoplasmic positivity in peripheral nerve.](http://images.novusbio.com/fullsize/SV2C-Antibody-Immunohistochemistry-Paraffin-NBP1-86239-img0005.jpg "Immunohistochemistry-Paraffin: SV2C Antibody [NBP1-86239] - Staining of human prostate shows moderate cytoplasmic positivity in peripheral nerve.")
![Immunohistochemistry-Paraffin: SV2C Antibody [NBP1-86239] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/SV2C-Antibody-Immunohistochemistry-Paraffin-NBP1-86239-img0003.jpg)
![Immunohistochemistry-Paraffin: SV2C Antibody [NBP1-86239] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/SV2C-Antibody-Immunohistochemistry-Paraffin-NBP1-86239-img0004.jpg)
![Immunohistochemistry-Paraffin: SV2C Antibody [NBP1-86239] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SV2C-Antibody-Immunohistochemistry-Paraffin-NBP1-86239-img0006.jpg)
![Western Blot: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71).](http://images.novusbio.com/fullsize/Vimentin-p-Ser71-Antibody-TM71-Western-Blot-NBP1-97515-img0001.jpg "Western Blot: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71).")
![Immunocytochemistry/Immunofluorescence: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) on 4% PFA fixed U251 cells with [pSer71]Vimentin, mAb (TM71).](http://images.novusbio.com/fullsize/Vimentin-p-Ser71-Antibody-TM71-Immunohistochemistry-NBP1-97515-img0002.jpg)
![Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Type: 293T. Antibody Dilution: 1.0ug/mlPITX3 is supported by BioGPS gene expression data to be expressed in HEK293T](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85481-img0002.jpg "Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Type: 293T. Antibody Dilution: 1.0ug/mlPITX3 is supported by BioGPS gene expression data to be expressed in HEK293T")
![Western Blot: PITX3 Antibody [NBP2-85481] - WB Suggested Anti-PITX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysate](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85481-img0001.jpg)
![Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Tissue: Human Hela Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85481-img0003.jpg)
![Western Blot: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32816_rabbit-vimentin-p-ser38-mab-je43-26-288202412442957.jpg "Western Blot: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Immunocytochemistry analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes, then treated with lambda pp for 1 hour labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32816_rabbit-vimentin-p-ser38-mab-je43-26-29202410234248.jpg)