| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73930 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2217SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92546-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13497-0.025ml | |||
![Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse liver tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-JM102-06-Immunohistochemistry-Paraffin-NBP2-67187-img0002.jpg "Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse liver tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse brain tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-JM102-06-Immunohistochemistry-Paraffin-NBP2-67187-img0001.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/5,000 dilution.Lane 1: A431 cell lysate (15 µg/Lane)Lane 2: Human liver tissue lysate (20 µg/Lane)Lane 3: Mouse heart tissue lysate (20 µg/Lane)Lane 4: Rat heart tissue lysate (20 µg/Lane)Lane 5: Mouse kidney tissue lysate (20 µg/Lane)Lane 6: Rat kidney tissue lysate (20 µg/Lane)Predicted band size: 46 kDaObserved band size: 55-100 kDaExposure time: 3 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-western-blot-28620249199..jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] -Analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/1,000 dilution.Lane 1: A431 cell lysate (no heat) (15 µg/Lane)Lane 2: Mouse kidney tissue lysate (no heat) (20 µg/Lane)Lane 3: Mouse heart tissue lysate (no heat) (20 µg/Lane)Lane 4: Rat kidney tissue lysate (no heat) (20 µg/Lane)Lane 5: Zebrafish tissue lysate (20 µg/Lane)Predicted band size: 46 kDaObserved band size: 55-100 kDaExposure time: 3 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-western-blot-286202492022..jpg)
![Flow Cytometry-beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] -Analysis of A431 cells labeling beta 2 Adrenergic Receptor.Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-flow-cytometry-28620249222..jpg)
![ICC/IF-beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of A431 cells labeling beta 2 Adrenergic Receptor with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-immunocytochemistry-immunofluorescence-286202492524..jpg)
![Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926] - Human breast cancer, using DDR2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/DDR2-Antibody-Immunohistochemistry-Paraffin-NBP2-14926-img0001.jpg "Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926] - Human breast cancer, using DDR2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: DDR2 Antibody [NBP2-14926] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/DDR2-Antibody-Western-Blot-NBP2-14926-img0004.jpg)
![Western Blot: DDR2 Antibody [NBP2-14926] - U87-MG whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDR2-Antibody-Western-Blot-NBP2-14926-img0005.jpg)
![Western Blot: DDR2 Antibody [NBP2-14926] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-91020242063328.jpg)
![DDR2 antibody [C2C3], C-term detects DDR2 protein at cell membrane by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DDR2 stained by DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:250.Red: Tau, an Axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-immunocytochemistry-immunofluorescence-2611202518261315.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and DDR2 (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-western-blot-2611202518384019.jpg)
![Western Blot: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROR beta.](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Western-Blot-NBP2-45610-img0013.jpg "Western Blot: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROR beta.")
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0012.jpg)
![Immunohistochemistry: ROR beta Antibody (OTI1G1) [NBP2-45610] - ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0001.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Adenocarcinoma of Human colon. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0002.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0003.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0004.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0005.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0006.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0007.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Human pancreas. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0008.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0009.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0010.jpg)
![Immunohistochemistry: ROR beta Antibody (1G1) [NBP2-45610] - Analysis of Carcinoma of Human prostate tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ROR-beta-Antibody-1G1-Immunohistochemistry-NBP2-45610-img0011.jpg)
![Western Blot: ATP5A Antibody [NBP2-38525] - Analysis using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar pattern to independent antibody NBP2-38470 (B).](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-38525-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ATP5A Antibody [NBP2-38525] - Analysis using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar pattern to independent antibody NBP2-38470 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human kidney.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0007.jpg)
![Western Blot: ATP5A Antibody [NBP2-38525] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-38525-img0002.jpg)
![Immunohistochemistry: ATP5A Antibody [NBP2-38525] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-NBP2-38525-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human colon, heart muscle, kidney and liver using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar protein distribution across tissues to independent antibody NBP2-38470 (B).](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human liver.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0005.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human colon.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38525-img0008.jpg)
![Western Blot: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis using PTH1R mAb against PTH1R (AA: 27-188)-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-4D2-Western-Blot-NBP1-51640-img0010.jpg "Western Blot: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis using PTH1R mAb against PTH1R (AA: 27-188)-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Generation of functional kidney spheroids using immortalized mouse primary cells. Representative images of mKidS immunostained for p-GP, OCT2, and PTHR. All data are shown as the means?+-?s.e.m. *p?less than ?0.05 compared with 2D or 3D control by unpaired Student's t-test. Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-53855-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-4D2-Immunocytochemistry-Immunofluorescence-NBP1-51640-img0011.jpg)
![Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis of human kidney tissues using PTH1R mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-4D2-Immunohistochemistry-Paraffin-NBP1-51640-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis of SK-BR-3 cells using PTH1R mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-4D2-Immunocytochemistry-Immunofluorescence-NBP1-51640-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) - BSA Free [NBP1-51640] - Generation of functional kidney spheroids using immortalized human primary cells. (a) Quantitative RT-PCR analysis of kidney-specific segment markers in hKidS compared with 2D controls (2D CTL) & human kidney lysates (hKidney). n = 8 for 2D CTL & hKidS & n = 3 for hKidney. (b) Western blotting analysis for renal multi-lineage marker in mouse kidney spheroids compared to 2D CTL & hKidney. The grouping of gels/blots cropped from different parts of the same gel. (c) Representative images for cell polarity of Zo-1, an apical membrane, & Na/K ATPase, a basolateral membrane marker in hKidS. Scale bar, 10 μm. (d) Representative images of human kidney spheroids (hKidS) at day 5 of maturation stained for the kidney-specific markers LTL, PNA, AQP2, & Nephrin. Human kidney was used as positive control for immunostaining of hKidS. (e) Responses to parathyroid hormone (PTH) of hKidS compared with 3D control spheroids (without maturation factors, CTL) & 2D cells. (f) GGT activity in hKidS compared with 3D CTL. (g) Temperature-dependent albumin-fluorescein uptake of hKidS compared to 3D CTL. n = 10 for 3D CTL & hKidS. (h,i) Relative mRNA expression of apical (h) & basolateral (i) transporters in hKidS compared to 2D cultured cells, hPTECs spheroids generated using the same protocol for hKidS (hPTECs-3D), & hKidney. n = 9 for 2D CTL & hKidS & n = 3 for hPTECs-3D & hKidney. (j) Representative images of hKidS immunostained for p-GP, OCT2, & PTHR. All data are shown as the means ± s.e.m. *p < 0.05 compared with 2D or 3D control by unpaired Student’s t-test. Scale bar, 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31772214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51640_mouse-monoclonal-pth1r-pthr1-antibody-4d2-310202416171477.jpg)
![Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - Western blot analysis of ApoER2 on different lysates using anti-ApoER2 antibody at 1/1,000 dilution.Positive control:Lane1: Mouse placentaLane2: SH-SY-5Y](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Western-Blot-NBP2-76911-img0001.jpg "Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - Western blot analysis of ApoER2 on different lysates using anti-ApoER2 antibody at 1/1,000 dilution.Positive control:Lane1: Mouse placentaLane2: SH-SY-5Y")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - ICC staining ApoER2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76911-img0004.jpg)
![Immunohistochemistry: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ApoER2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunohistochemistry-NBP2-76911-img0005.jpg)
![Flow Cytometry: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - Flow cytometric analysis of SH-SY-5Y cells with ApoER2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Flow-Cytometry-NBP2-76911-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - ICC staining ApoER2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76911-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - ICC staining ApoER2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76911-img0003.jpg)
![Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2217] - Detection of ApoER2 in mouse brain membrane lysate using NB 100-2217. ECL exposure, 1 min.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Western-Blot-NB100-2217-img0005.jpg "Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2217] - Detection of ApoER2 in mouse brain membrane lysate using NB 100-2217. ECL exposure, 1 min.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2217] - Antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2217-img0006.jpg)
![Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DDR2-Antibody-HL1107-Western-Blot-NBP3-13724-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DDR2-Antibody-HL1107-Western-Blot-NBP3-13724-img0002.jpg)
![Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-91020242011494.jpg)
![<b>Orthogonal Strategies Validation. </b> Mouse tissues.DDR2 stained by DDR2 antibody [HL1107] diluted at 1:16000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-immunohistochemistry-paraffin-317202591535..png)
![DDR2 antibody [HL1107] detects DDR2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.DDR2 stained by DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:16000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-immunohistochemistry-paraffin-2611202518404824.jpg)
![U87-MG whole cell and membrane extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-western-blot-2611202518475722.jpg)
![DDR2 antibody [HL1107] detects DDR2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.DDR2 stained by DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:16000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-immunohistochemistry-paraffin-261120251843459.jpg)
![Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-2120249351339.jpg "Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-2120249362767.jpg)
![Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-2120249422655.jpg)
![Immunohistochemistry-Paraffin: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - HEY1 antibody [HL2367] detects HEY1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. HEY1 stained by HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-212024943557.jpg)
![Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-2120249371561.jpg)
![Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-212024943284.jpg)
![Western Blot: DSPP Antibody - Azide and BSA Free [NBP2-92546] - Western blot analysis of various lysates using DSPP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92546_rabbit-polyclonal-dspp-antibody-4220251430494.jpg%3Ft=1711070358 "Western Blot: DSPP Antibody - Azide and BSA Free [NBP2-92546] - Western blot analysis of various lysates using DSPP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0009.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0002.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0003.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-288202414233110.jpg "Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241435230.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33140, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241492693.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29202410484841.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33140, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-392024884679.jpg)