| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33102 | |||
| IHC-Prediluted | NBP2-44724 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90815-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89762-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05210-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:100 overnight at 4C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0008.jpg "Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:100 overnight at 4C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI.")
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Indirect staining of rat neuronal/glial cell culture.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATP Synthase beta monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ATP Synthase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATP Synthase beta monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ATP Synthase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0007.jpg)
![Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of rat neuronal/glial cell extract.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunoprecipitation-NB600-1171-img0010.jpg)
![Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta was performed on THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of an ATP Synthase beta monoclonal antibody overnight at 4C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted with 6X Laemmli non-reducing sample buffer. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:1000 overnight at 4C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunoprecipitation-NB600-1171-img0009.jpg)
![Western Blot: NF-H Antibody (NF421) [NBP2-29629] - Western Blot Analysis of Human Brain Tissue Lysate using NF-H antibody (NF421).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Western-Blot-NBP2-29629-img0007.jpg "Western Blot: NF-H Antibody (NF421) [NBP2-29629] - Western Blot Analysis of Human Brain Tissue Lysate using NF-H antibody (NF421).")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody (NF421) [NBP2-29629] - Immunofluorescence staining of HEK293 cells using NF-H Antibody (NF421) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunocytochemistry-Immunofluorescence-NBP2-29629-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with NF-H Antibody (NF421).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0006.jpg)
![Flow Cytometry: NF-H Antibody (NF421) [NBP2-29629] - Flow Cytometric Analysis of HEK293 cells using NF-H Antibody (NF421) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Flow-Cytometry-NBP2-29629-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (RT-91).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded human cerebellum stained with neurofilament monoclonal Antibody (RT-97)](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0003.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded human cerebellum stained with Neurofilament NF421 antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0001.jpg)
![Western Blot: ATPB Antibody [NBP1-90815] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90815-img0015.jpg "Western Blot: ATPB Antibody [NBP1-90815] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: ATPB Antibody [NBP1-90815] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90815-img0007.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATPB Antibody [NBP1-90815] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATP5B antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90815-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90815 (A) shows similar protein distribution across tissues to independent antibody NBP1-90816 (B).](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0016.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0018.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0019.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0021.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0016.jpg "Western Blot: ATP5A Antibody [NBP2-15512] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: ATP5A Antibody [NBP2-15512] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:1000.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0004.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted by 1:2000.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0005.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0010.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0015.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Colon carcinoma. ATP5A1 antibody [C2C3], C-term dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse stomach. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse lung. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0007.jpg)
![ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (NBP2-15512) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15512_rabbit-polyclonal-atp5a-antibody-immunohistochemistry-frozen-26112025197457.jpg)
![ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (NBP2-15512) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15512_rabbit-polyclonal-atp5a-antibody-immunocytochemistry-immunofluorescence-2611202518384018.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19320-img0015.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Mouse liver. LDHA stained by LDHA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Immunohistochemistry-Paraffin-NBP2-19320-img0017.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Human lung papillory adenocarcinoma, using LDHA antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-19320-img0002.jpg)
![Western Blot: Glutaminase Antibody (5C4) [H00002744-M01] - Western Blot analysis of GLS expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/Glutaminase-Antibody-5C4-Western-Blot-H00002744-M01-img0001.jpg "Western Blot: Glutaminase Antibody (5C4) [H00002744-M01] - Western Blot analysis of GLS expression in Hela NE ( Cat # L013V3 ).")
![Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01] - Analysis of monoclonal antibody to GLS on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-5C4-Immunocytochemistry-Immunofluorescence-H00002744-M01-img0004.jpg)
![ELISA: Glutaminase Antibody (5C4) [H00002744-M01] - Detection limit for recombinant GST tagged GLS is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-5C4-ELISA-H00002744-M01-img0006.jpg)
![Western Blot: ATP5A Antibody (6M3B8) [NBP3-15355] - Analysis of extracts of various cell lines, using ATP5A Rabbit mAb (NBP3-15355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATP5A-Antibody-6M3B8-Western-Blot-NBP3-15355-img0006.jpg "Western Blot: ATP5A Antibody (6M3B8) [NBP3-15355] - Analysis of extracts of various cell lines, using ATP5A Rabbit mAb (NBP3-15355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Simple Western: ATP5A Antibody (6M3B8) [NBP3-15355] - ATP5A Antibodies (NBP3-15355), ProteinSimple Western Blot on Jess Instrument; 1, 0.5, and 0.2 microgram of human brain tissue lysate was tested with the antibodies diluted 20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-simple-western-204202311391.jpg)
![Immunocytochemistry/Immunofluorescence: ATP5A Antibody (6M3B8) [NBP3-15355] - Confocal imaging of NIH/3T3 cells using ATP5A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-immunocytochemistry-immunofluorescence-412202412213..png)
![Immunohistochemistry-Paraffin: ATP5A Antibody (6M3B8) [NBP3-15355] -Rat brain tissue using ATP5A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-immunohistochemistry-paraffin-412202412333..png)
![Immunohistochemistry-Paraffin: ATP5A Antibody (6M3B8) [NBP3-15355] - Mouse brain tissue using ATP5A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-immunohistochemistry-paraffin-412202412451..png)
![Immunocytochemistry/Immunofluorescence: ATP5A Antibody (6M3B8) [NBP3-15355] - Confocal imaging of HeLa cells using ATP5A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-immunocytochemistry-immunofluorescence-412202412651..png)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517223956.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody (6M3B8) [ATP5A] - Confocal imaging of paraffin-embedded Mouse brain tissue using ATP5A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-5220251718967.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody (6M3B8) [ATP5A] - Confocal imaging of NIH/3T3 cells using ATP5A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517251916.jpg)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517235715.jpg)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-522025172603.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody (6M3B8) [ATP5A] - Confocal imaging of paraffin-embedded Rat brain tissue using ATP5A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517223923.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody (6M3B8) [ATP5A] - Confocal imaging of HeLa cells using ATP5A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517202976.jpg)
![Immunoprecipitation: ATP5A Antibody (6M3B8) [ATP5A] - Immunoprecipitation analysis of 600 ug extracts of Mouse heart using 3 ug ATP5A antibody . Western blot was performed from the immunoprecipitate using ATP5A antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517162373.jpg)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517152767.jpg)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517235771.jpg)
![Immunohistochemistry: ATP5A Antibody (6M3B8) [ATP5A] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using ATP5A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15355_rabbit-atp5a-mab-6m3b8-52202517202985.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0005.jpg "Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-326). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-326-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0002.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of GAPDH in nu/nu Mouse cells. 5% BSA in PBS, 1:5,000. Secondary: Goat anti-Rabbit IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0003.jpg)
![Simple Western: GAPDH Antibody [NB300-326] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-326-img0001.jpg)
![Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Western-Blot-NBP1-05210-img0006.jpg "Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody (9B12) [NBP1-05210] - Mixed neuron/glial cultures stained with NBP1-05210 (red) and rabbit GFAP antibody NB300-141 (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue).](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Immunocytochemistry-Immunofluorescence-NBP1-05210-img0003.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody (9B12) [NBP1-05210] - Analysis of a rat brain coronal section of the third ventricle stained with mouse monoclonal antibody to phosphorylated NF-H, dilution 1:5,000 (Green). Hoechst staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibody. The antibody is a robust marker of the axons of neuronal cells.](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Immunohistochemistry-Free-Floating-NBP1-05210-img0005.jpg)
![Wallerian degeneration and mitochondrial degeneration in axon for rat’s distal nerve stump. a, Representative images of toluidine blue staining, immunohistochemistry (NF-H), electron microscopy, before (pre) and 1, 2, and 14 d after axotomy. Immunohistochemistry was obtained the same results for three individuals. Scale bar, * 10 μm, ** 2 μm. Arrows, myelinated axon; arrowheads, demyelinated Schwann cell. b, Quantification of the myelinated axon. c, d, Quantification of the G-ratio [c: cumulative G-ratio per individual, d: scatter plot, Spearman’s rank correlation coefficient and p values showing G-ratio of individual myelinated axons against axon diameter (n = 400)]. The histologic WD was observed significantly from 2 d after axotomy. In axons, before (pre) and 1 and 2 d after axotomy were evaluated, except for after 14 d with completely collapsed axon morphology (n = 4 rat per group). e, Representative images of mitochondrial findings, before (pre) and 1, 2, and 14 d after axotomy. Evaluation was performed in axons and Schwann cells separately. Scale bar, * 200 nm. f, Violin plot of mitochondrial diameter (nm; n = 200). Cumulative mitochondria diameter per individual are also shown (n = 4 rat per group). g, Quantitative analysis of atypical mitochondria (%; n = 4 rat per group). In axons, mitochondria were significantly degenerated from 1 d after axotomy, whereas in Schwann cells, mitochondria were not significantly degenerated until 14 d after axotomy. All histologic evaluations were performed 3 mm distal to the sectional end and corresponding uninjured nerve. Error bars indicate SD; *p < 0.05, ***p < 0.001, one-way ANOVA followed by the Tukey’s post hoc test (for comparison of axons and Schwann cells, respectively). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36894321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05210_mouse-monoclonal-nf-h-antibody-9b12-immunocytochemistry-immunofluorescence-132202617595724.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis. Sample: Paraffin-embedded dog tissues. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249383165.jpg "Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis. Sample: Paraffin-embedded dog tissues. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249351313.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-21202494086.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249362757.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis. Sample: Paraffin-embedded cat brain. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249351325.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-212024943287.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249415919.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Non-transfected (-) and transfected (+) A549 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249425435.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249403899.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-212024941591.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (9A1O2) [NBP3-15354] - Immunohistochemistry of paraffin-embedded human esophageal cancer using ATPB Rabbit mAb (NBP3-15354) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATPB-Antibody-9A1O2-Immunohistochemistry-Paraffin-NBP3-15354-img0002.jpg "Immunohistochemistry-Paraffin: ATPB Antibody (9A1O2) [NBP3-15354] - Immunohistochemistry of paraffin-embedded human esophageal cancer using ATPB Rabbit mAb (NBP3-15354) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: ATPB Antibody (9A1O2) [NBP3-15354] - Immunohistochemistry of paraffin-embedded rat kidney using ATPB Rabbit mAb (NBP3-15354) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATPB-Antibody-9A1O2-Immunohistochemistry-Paraffin-NBP3-15354-img0001.jpg)
![Western Blot: ATPB Antibody (9A1O2) [NBP3-15354] - analysis of extracts of various cell lines, using ATPB antibody at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-9a1o2-western-blot-510202312496..jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (9A1O2) [NBP3-15354] - HepG2 cells using ATPB Rabbit mAb (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-9a1o2-immunocytochemistry-immunofluorescence-5102023125019..jpg)
![Immunohistochemistry: ATPB Antibody (1H2W2) [ATPB] - Immunohistochemistry analysis of ATPB in paraffin-embedded human colon carcinoma tissue using ATPB Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-1h2w2-52202517162363.jpg)
![Immunohistochemistry: ATPB Antibody (1H2W2) [ATPB] - Immunohistochemistry analysis of ATPB in paraffin-embedded Human lung adenocarcinoma tissue using ATPB Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-1h2w2-52202517213571.jpg)
![Immunohistochemistry: ATPB Antibody (1H2W2) [ATPB] - Immunohistochemistry analysis of ATPB in paraffin-embedded mouse kidney tissue using ATPB Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-1h2w2-52202517162317.jpg)
![Immunohistochemistry: ATPB Antibody (1H2W2) [ATPB] - Immunohistochemistry analysis of ATPB in paraffin-embedded human thyroid cancer tissue using ATPB Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-1h2w2-52202517251947.jpg)
![Immunocytochemistry/ Immunofluorescence: ATPB Antibody (1H2W2) [ATPB] - Confocal imaging of NIH/3T3 cells using ATPB Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-1h2w2-52202517273562.jpg)
![Immunohistochemistry: ATPB Antibody (1H2W2) [ATPB] - Immunohistochemistry analysis of ATPB in paraffin-embedded rat kidney tissue using ATPB Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-1h2w2-52202517152737.jpg)
![Immunocytochemistry/ Immunofluorescence: ATPB Antibody (1H2W2) [ATPB] - Confocal imaging of Hep G2 cells using ATPB Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-1h2w2-52202517172867.jpg)
![Immunohistochemistry: ATPB Antibody (1H2W2) [ATPB] - Immunohistochemistry analysis of ATPB in paraffin-embedded human liver cancer tissue using ATPB Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-1h2w2-52202517162337.jpg)
![Immunohistochemistry: ATPB Antibody (1H2W2) [ATPB] - Immunohistochemistry analysis of ATPB in paraffin-embedded human liver tissue using ATPB Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-1h2w2-52202517162327.jpg)