| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08936 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77179-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43924 | |||
| BSA Free | NBP2-52867-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44056 | |||
| BSA Free | NBP2-61628-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44057 | |||
| BSA Free | NBP2-61629-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24559-25ul | |||
| Azide and BSA Free | NBP3-43869 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54544-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70511 | |||
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0005.jpg "Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0003.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human A375 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human melanoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0002.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Analysis in EL4 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.Red: ZEB2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202419333477.gif)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, and then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0012.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - The protein levels of EMT-TFs were compared between carcinoma cell lines and non- or less-malignant tumor cell lines by using Western-blot analysis. The soft agar colony transformation or migration assay was referred to distinguish malignant degree of the experimental cancer cell lines (data not shown). Malignant degree: prostate cancer cell lines, P69 < M12; breast cancer cell lines, MCF7 < MD-MBA-231; lung cancer cell lines, A549 < H1299; liver cancer cell lines, HepG2 < SMMC7721. 'kD' (kilo-dalton) means the molecular weight of protein. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0013.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415392525.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415334920.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415395947.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241535644.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Functional domains of Fbxo45 for ubiquitination of Zeb2A. Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, & then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. B. Bacterially expressed GST or GST–Fbxo45, -Fbxo45 delta F-box, -Fbxo45 delta mid, or -Fbxo45 delta SPRY fusion proteins & Glutathione-Sepharose beads were incubated with lysates of HeLa cells transfected with Zeb2, Snai1 or Snai2, respectively. The proteins associated with GST–tagged Fbxo45 forms, bound on the Glutathione-Sepharose beads were washed five times with the RIPA buffer before immunoblotting. C. Lysates from 293T cells transfected Flag-Zeb2 with HA-Fbxo45, -Fbxo45 delta mid, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were immunoprecipitated with anti-Flag or anti-HA antibodies, & immunoprecipitates were resolved by SDS-PAGE for Western-blot analysis. D. U2OS cells transfected with Flag-Zeb2 & HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were stained using the primary antibodies of anti-Flag M2 or anti-HA, & the second antibodies of Alexa Fluor 568 anti-mouse or Alexa Fluor 488 anti-Rabbit, respectively. Scale: 25μm. E-I. Zeb2 ubiquitination assays by using IP expriments under different conditions: Flag-Zeb2 with or without HA-Ub (E); Flag-Zeb2 with HA-Ub WT, K48R or K63R (F); Flag-Zeb2 & HA-Ub K48-only with or without myc-Fbox45 (G); Flag-Zeb2 & HA-Ub K48-only with siRNA control, siRNAs for Fbxo45 or siRNAs for Pam (H); Flag-Zeb2 & HA-Ub with HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY (I). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416163752.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416175234.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241616011.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420285893.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420272496.jpg)
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Knockout-Validated-NBP2-52867-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0010.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human skin shows distinct positivity in fibroblasts.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0005.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa] Lane 2: Human tonsil tissue lysate](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0006.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human cerebellum shows immunoreactivity in the endothelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human kidney shows strong immunoreactivity in renal glomeruli but not tubuli cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human prostate shows strong immunoreactivity in the connective tissue.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0004.jpg)
![Western Blot: beta-Catenin Antibody (CL3689) [NBP2-61628] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Western-Blot-NBP2-61628-img0010.jpg "Western Blot: beta-Catenin Antibody (CL3689) [NBP2-61628] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of A-431 cells using the Anti-CTNNB1 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunocytochemistry-Immunofluorescence-NBP2-61628-img0011.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human skeletal muscle shows absence of immunoreactivity in muscle fibers (negative control).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-Paraffin-NBP2-61628-img0008.jpg)
![Western Blot: beta-Catenin Antibody (CL3689) [NBP2-61628] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Western-Blot-NBP2-61628-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of U-251 cells using the Anti-CTNNB1 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunocytochemistry-Immunofluorescence-NBP2-61628-img0012.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human endometrium shows strong membranous immunoreactivity in glandular epithelium.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-NBP2-61628-img0001.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human kidney shows strong membranous positivity in renal tubules and glomerulus cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-NBP2-61628-img0002.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human duodenum shows strong membranous immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-NBP2-61628-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human cervix shows strong membranous positivity in epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-NBP2-61628-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human breast cancer shows membranous immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-Paraffin-NBP2-61628-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human prostate cancer shows membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-Paraffin-NBP2-61628-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human renal cancer shows membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-Paraffin-NBP2-61628-img0007.jpg)
![Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-GT2169-Western-Blot-NBP3-13530-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.")
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Wild-type (WT) and beta-Catenin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-GT2169-Western-Blot-NBP3-13530-img0004.jpg)
![Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP3-13530) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-91020242091497.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (GT2169) [NBP3-13530] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta-Catenin stained by beta-Catenin antibody (NBP3-13530) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-910202420131215.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin protein stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunocytochemistry-immunofluorescence-2611202518375021.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-261120251843450.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded human pancreas.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-26112025196218.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-26112025184740.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded human appendix.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-261120251829266.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-western-blot-2611202518372424.jpg)
![Western Blot: beta-Catenin Antibody (CL3691) [NBP2-61629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Western-Blot-NBP2-61629-img0011.jpg "Western Blot: beta-Catenin Antibody (CL3691) [NBP2-61629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of A-431 cells using the Anti-CTNNB1 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunocytochemistry-Immunofluorescence-NBP2-61629-img0012.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human skeletal muscle shows absence of immunoreactivity in muscle fibers (negative control).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-Paraffin-NBP2-61629-img0009.jpg)
![Western Blot: beta-Catenin Antibody (CL3691) [NBP2-61629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Western-Blot-NBP2-61629-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of U-251 cells using the Anti-CTNNB1 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunocytochemistry-Immunofluorescence-NBP2-61629-img0013.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human small intestine shows strong membranous immunoreactivity in the epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-NBP2-61629-img0001.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human cervix shows strong membranous positivity in epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-NBP2-61629-img0002.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human prostate shows membranous immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-NBP2-61629-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human fallopian tube shows strong membranous immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-NBP2-61629-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human placenta shows strong membranous positivity in trophoblast.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-Paraffin-NBP2-61629-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human prostate cancer shows membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-Paraffin-NBP2-61629-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human breast cancer shows membranous immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-Paraffin-NBP2-61629-img0007.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3691) [NBP2-61629] - Staining of human renal cancer shows membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3691-Immunohistochemistry-Paraffin-NBP2-61629-img0008.jpg)
![Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (15B8).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Western-Blot-NBP2-53358-img0004.jpg "Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (15B8).")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (15B8) [NBP2-53358] - Confocal immunofluorescence image of HeLa cells using beta-Catenin Antibody (15B8) labeled is Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Immunocytochemistry-Immunofluorescence-NBP2-53358-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (15B8) [NBP2-53358] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin (p120) Monoclonal Antibody (15B8).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Immunohistochemistry-Paraffin-NBP2-53358-img0001.jpg)
![Western Blot: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Western-Blot-NBP2-46401-img0008.jpg "Western Blot: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.")
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0007.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0001.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0002.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (2F10) [NBP2-46401] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-2F10-Immunohistochemistry-NBP2-46401-img0006.jpg)
![Western Blot: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Western-Blot-NBP2-70511-img0001.jpg "Western Blot: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.")
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0007.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0002.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0006.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI2F10) - Azide and BSA Free [NBP2-70511] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI2F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70511-img0008.jpg)
![Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of CTNNB1 expression in transfected 293T cell line by CTNNB1 monoclonal antibody (M02), clone 1C9.Lane 1: CTNNB1 transfected lysate(85.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-1C9-Western-Blot-H00001499-M02-img0009.jpg "Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of CTNNB1 expression in transfected 293T cell line by CTNNB1 monoclonal antibody (M02), clone 1C9.Lane 1: CTNNB1 transfected lysate(85.5 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (1C9) [H00001499-M02] - PC-3 MM2 cells were stained with CTNNB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-1C9-Immunocytochemistry-Immunofluorescence-H00001499-M02-img0006.jpg)
![Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of CTNNB1 over-expressed 293 cell line, cotransfected with CTNNB1 Validated Chimera RNAi ( Cat # H00001499-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CTNNB1 monoclonal antibody (M02), clone 1C9 (Cat # H00001499-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-1C9-Western-Blot-H00001499-M02-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of monoclonal antibody to CTNNB1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-1C9-Immunocytochemistry-Immunofluorescence-H00001499-M02-img0005.jpg)
![Proximity Ligation Assay: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of protein-protein interactions between GSK3B and CTNNB1. HeLa cells were stained with anti-GSK3B rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-1C9-Proximity-Ligation-Assay-H00001499-M02-img0003.jpg)
![Proximity Ligation Assay: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of protein-protein interactions between FLT1 and CTNNB1. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-1C9-Proximity-Ligation-Assay-H00001499-M02-img0004.jpg)
![ELISA: beta-Catenin Antibody (1C9) [H00001499-M02] - Detection limit for recombinant GST tagged CTNNB1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-1C9-ELISA-H00001499-M02-img0008.jpg)