| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83277-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38258-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57689-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87885-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92713-50uL | |||
![Immunocytochemistry/Immunofluorescence: CNTF Antibody [NBP1-83277] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CNTF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83277-img0006.jpg "Immunocytochemistry/Immunofluorescence: CNTF Antibody [NBP1-83277] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/CNTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83277-img0010.jpg)
![Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/CNTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83277-img0008.jpg)
![Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/CNTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83277-img0009.jpg)
![Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Skin shows very weak cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CNTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83277-img0011.jpg)
![Western Blot: CRMP5 Antibody [NBP1-33419] - Mouse tissue extract (5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CRMP5-Antibody-Western-Blot-NBP1-33419-img0010.jpg "Western Blot: CRMP5 Antibody [NBP1-33419] - Mouse tissue extract (5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CRMP5 Antibody [NBP1-33419] - Paraffin-embedded mouse lung. CRMP5 antibody [N1C1] diluted at 1:500.](http://images.novusbio.com/fullsize/CRMP5-Antibody-Immunohistochemistry-Paraffin-NBP1-33419-img0008.jpg)
![Western Blot: CRMP5 Antibody [NBP1-33419] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CRMP5-Antibody-Western-Blot-NBP1-33419-img0005.jpg)
![Immunohistochemistry-Paraffin: CRMP5 Antibody [NBP1-33419] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CRMP5-Antibody-Immunohistochemistry-Paraffin-NBP1-33419-img0002.jpg)
![CRMP5 antibody [N1C1] detects CRMP5 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: CRMP5 protein stained by CRMP5 antibody [N1C1] (NBP1-33419) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33419_rabbit-polyclonal-crmp5-antibody-immunocytochemistry-immunofluorescence-2611202518411321.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta-III Tubulin antibody [GT1338] detects beta-III Tubulin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta-III Tubulin protein stained by beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Immunocytochemistry-Immunofluorescence-NBP3-13505-img0002.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (NBP3-13505) diluted by 1:1000.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0001.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0003.jpg)
![beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13505_mouse-beta-iii-tubulin-mab-gt1338-immunohistochemistry-paraffin-2611202518393116.jpg)
![Immunohistochemistry: Recoverin Antibody [NBP3-38258] - Immunohistochemistry analysis of paraffin-embedded Mouse retina using Recoverin Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38258_rabbit-recoverin-pab-912202414163065.jpg "Immunohistochemistry: Recoverin Antibody [NBP3-38258] - Immunohistochemistry analysis of paraffin-embedded Mouse retina using Recoverin Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Recoverin Antibody [NBP3-38258] - Immunohistochemistry analysis of paraffin-embedded Rat retina using Recoverin Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38258_rabbit-recoverin-pab-912202415161810.jpg)
![Western Blot: Recoverin Antibody [NBP3-38258] - Western blot analysis of various lysates using Recoverin Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-38258_rabbit-recoverin-pab-9122024159535.jpg)
![Immunocytochemistry/ Immunofluorescence: Recoverin Antibody [NBP3-38258] - Immunofluorescence analysis of paraffin-embedded rat eye using Recoverin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38258_rabbit-recoverin-pab-912202414123816.jpg)
![Immunocytochemistry/ Immunofluorescence: Recoverin Antibody [NBP3-38258] - Immunofluorescence analysis of paraffin-embedded mouse eye using Recoverin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38258_rabbit-recoverin-pab-91220241519545.jpg)
![Western Blot: GAP-43 Antibody (8E7C8) [NBP3-15636] - Western blot analysis of extracts of various cell lines, using GAP-43 antibody (NBP3-15636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/GAP-43-Antibody-8E7C8-Western-Blot-NBP3-15636-img0002.jpg "Western Blot: GAP-43 Antibody (8E7C8) [NBP3-15636] - Western blot analysis of extracts of various cell lines, using GAP-43 antibody (NBP3-15636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody (8E7C8) [GAP-43] - Confocal imaging of paraffin-embedded Rat brain tissue using GAP-43 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15636_rabbit-gap-43-mab-8e7c8-52202517304971.jpg)
![Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody (8E7C8) [GAP-43] - Confocal imaging of paraffin-embedded Mouse brain tissue using GAP-43 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15636_rabbit-gap-43-mab-8e7c8-52202517352710.jpg)
![Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody (8E7C8) [GAP-43] - Confocal imaging of paraffin-embedded Mouse eye tissue using GAP-43 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15636_rabbit-gap-43-mab-8e7c8-52202517315583.jpg)
![Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody (8E7C8) [GAP-43] - Confocal imaging of paraffin-embedded Rat eye tissue using GAP-43 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15636_rabbit-gap-43-mab-8e7c8-52202517333828.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-28820241423313.jpg "Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29820241435223.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29202410484898.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-39202488464.jpg)
![Immunocytochemistry/Immunofluorescence: LINGO-1 Antibody [NBP2-57689] - Staining of human cell line HEK 293 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LINGO-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57689-img0001.jpg "Immunocytochemistry/Immunofluorescence: LINGO-1 Antibody [NBP2-57689] - Staining of human cell line HEK 293 shows localization to plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: LINGO-1 Antibody [NBP2-57689] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/LINGO-1-Antibody-Immunohistochemistry-Paraffin-NBP2-57689-img0005.jpg)
![Immunohistochemistry-Paraffin: LINGO-1 Antibody [NBP2-57689] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/LINGO-1-Antibody-Immunohistochemistry-Paraffin-NBP2-57689-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87885-img0008.jpg "Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
![Western Blot: GAP-43 Antibody (1E3) [NBP1-92713] - Western blot of whole rat spinal cord lysates probed with GAP-43 antibody (1E3) antibody to GAP-43 in lane 16 and another similar antibody in lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. The strong single band running at about 43kDa corresponds to GAP-43.](http://images.novusbio.com/fullsize/GAP-43-Antibody-1E3-Western-Blot-NBP1-92713-img0002.jpg "Western Blot: GAP-43 Antibody (1E3) [NBP1-92713] - Western blot of whole rat spinal cord lysates probed with GAP-43 antibody (1E3) antibody to GAP-43 in lane 16 and another similar antibody in lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. The strong single band running at about 43kDa corresponds to GAP-43.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (1E3) [NBP1-92713] - Mixed neuronal cultures stained with GAP-43 antibody (1E3) (green), a rabbit antibody to microtubule associated protein 2 (red) and DNA (blue). The GAP-43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.](http://images.novusbio.com/fullsize/GAP-43-Antibody-1E3-Immunocytochemistry-Immunofluorescence-NBP1-92713-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RTN1 Antibody (MON162) [NBP1-97677] - Immunostaining of NCI-H82 variant small cell lung cancer](http://images.novusbio.com/fullsize/RTN1-Antibody-MON162-Immunocytochemistry-Immunofluorescence-NBP1-97677-img0001.jpg "Immunocytochemistry/Immunofluorescence: RTN1 Antibody (MON162) [NBP1-97677] - Immunostaining of NCI-H82 variant small cell lung cancer")
![Immunohistochemistry-Frozen: RTN1 Antibody (MON162) [NBP1-97677] - Immunohistochemistry on frozen section of swine brain](http://images.novusbio.com/fullsize/RTN1-Antibody-MON162-Immunohistochemistry-Frozen-NBP1-97677-img0002.jpg)
![Western Blot: Brevican Antibody [NBP2-15616] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Brevican antibody [N1N2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Brevican-Antibody-Western-Blot-NBP2-15616-img0004.jpg "Western Blot: Brevican Antibody [NBP2-15616] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Brevican antibody [N1N2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Brevican Antibody [NBP2-15616] - Human breast cancer, using Brevican antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Brevican-Antibody-Immunohistochemistry-Paraffin-NBP2-15616-img0002.jpg)
![Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: THP-1 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-288202412442993.jpg "Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: THP-1 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32860) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-298202414333682.jpg)
![Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling LEDGF with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32860, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-29820241420980.jpg)
![Immunocytochemistry/ Immunofluorescence: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunocytochemistry analysis of SH-SY5Y cells labeling LEDGF with Mouse anti-LEDGF antibody (NBP3-32860) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-LEDGF antibody (NBP3-32860) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-29202410234271.jpg)