| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61786-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48318-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59329-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13497-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59322-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89196-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54488-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27174SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92692SS | |||
![Western Blot: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis using HTR3A mAb against human HTR3A (AA: extra 24-157) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/5-HT3A-Antibody-4D5E7-Western-Blot-NBP2-61786-img0005.jpg "Western Blot: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis using HTR3A mAb against human HTR3A (AA: extra 24-157) recombinant protein. (Expected MW is 41.9 kDa)")
![Immunocytochemistry/Immunofluorescence: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis of Hela cells using HTR3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-4D5E7-Immunocytochemistry-Immunofluorescence-NBP2-61786-img0003.jpg)
![Immunohistochemistry-Paraffin: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis of cervical cancer tissues using HTR3A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-4D5E7-Immunohistochemistry-NBP2-61786-img0004.jpg)
![Flow Cytometry: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis of Hela cells using HTR3A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/5-HT3A-Antibody-4D5E7-Flow-Cytometry-NBP2-61786-img0002.jpg)
![Western Blot: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis using HTR3A mAb against HEK293 (1) and HTR3A (AA: extra 24-157)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-4D5E7-Western-Blot-NBP2-61786-img0006.jpg)
![ELISA: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/5-HT3A-Antibody-4D5E7-ELISA-NBP2-61786-img0001.jpg)
![Western Blot: TRP7 Antibody (N64A/36) [NBP1-48318] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Western-Blot-NBP1-48318-img0005.jpg "Western Blot: TRP7 Antibody (N64A/36) [NBP1-48318] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: TRP7 Antibody (N64A/36) [NBP1-48318] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC7 Antibody (D) Composite.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunocytochemistry-Immunofluorescence-NBP1-48318-img0007.jpg)
![Immunohistochemistry: TRP7 Antibody (N64A/36) [NBP1-48318] - Immunohistochemistry analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunohistochemistry-NBP1-48318-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TRP7 Antibody (N64A/36) [NBP1-48318] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining .](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunocytochemistry-Immunofluorescence-NBP1-48318-img0004.jpg)
![Immunohistochemistry-Paraffin: TRP7 Antibody (N64A/36) [NBP1-48318] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunohistochemistry-Paraffin-NBP1-48318-img0001.jpg)
![Western Blot: VGLUT1 Antibody (S28-9) [NBP2-59329] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:1000.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-S28-9-Western-Blot-NBP2-59329-img0005.jpg "Western Blot: VGLUT1 Antibody (S28-9) [NBP2-59329] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-S28-9-Immunocytochemistry-Immunofluorescence-NBP2-59329-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) VGLUT1 Antibody. (D) Hoechst (E) Composite.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-S28-9-Immunocytochemistry-Immunofluorescence-NBP2-59329-img0007.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of colon cancer tissues using FOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Immunohistochemistry-NBP2-37492-img0005.jpg "Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of colon cancer tissues using FOS mouse mAb with DAB staining.")
![Western Blot: c-Fos Antibody (2G2) [NBP2-37492] - Western blot analysis using FOS mouse mAb against HeLa (1), and HeLa (2) cell lysate.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Western-Blot-NBP2-37492-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of cerebellum tissues using FOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Immunohistochemistry-NBP2-37492-img0006.jpg)
![Flow Cytometry: c-Fos Antibody (2G2) [NBP2-37492] - Flow cytometric analysis of HeLa cells using FOS mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Flow-Cytometry-NBP2-37492-img0004.jpg)
![Western Blot: c-Fos Antibody (2G2) [NBP2-37492] - Western blot analysis using FOS mAb against human FOS (AA: 116-298) recombinant protein. (Expected MW is 45.8 kDa)](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Western-Blot-NBP2-37492-img0002.jpg)
![ELISA: c-Fos Antibody (2G2) [NBP2-37492] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-ELISA-NBP2-37492-img0001.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0009.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0002.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0003.jpg)
![Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322] - Western Blot analysis of Rat brain membrane lysate showing detection of CACNA1G Calcium Channel protein using Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Primary Antibody: Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody (NBP2-59322) at 1:1000.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Western-Blot-NBP2-59322-img0005.jpg "Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322] - Western Blot analysis of Rat brain membrane lysate showing detection of CACNA1G Calcium Channel protein using Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Primary Antibody: Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody (NBP2-59322) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) CACNA1G Antibody (D) Composite.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) CACNA1G Antibody. (D) Composite.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0007.jpg)
![Western Blot: Mouse Monoclonal CACNA1G Antibody (S178A-9) [NBP2-59322] - Western blot using lysates from human primary astrocytes or IGR39 melanoma cell line. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-59322_mouse-monoclonal-cacna1g-antibody-s178a-9-western-blot-1122023151422..png)
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)
![Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-288202414191539.jpg "Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33093) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-29820241433367.jpg)
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33093, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-298202414304144.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-2920241114843.jpg)
![Flow Cytometry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Flow cytometric analysis of Hela cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33093, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-392024884612.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0002.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human testis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0001.jpg)
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Western Blot Analysis of human HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1582).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Western-Blot-NBP2-53236-img0007.jpg "Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Western Blot Analysis of human HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1582).")
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta 3 Antibody (SPTBN2/1582).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Immunohistochemistry-Paraffin-NBP2-53236-img0005.jpg)
![Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Flow Cytometric Analysis of HeLa cells using Spectrin beta 3 Antibody (SPTBN2/1582). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Flow-Cytometry-NBP2-53236-img0004.jpg)
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Analysis of HeLa and 293 Cell Lysates using Spectrin beta III Monoclonal Antibody (SPTBN2/1582).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Western-Blot-NBP2-53236-img0001.jpg)
![Protein Array: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta 3 Antibody (SPTBN2/1582). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Protein-Array-NBP2-53236-img0006.jpg)
![Western Blot: HES5 Antibody [NBP2-27174] - analysis of HES5 in mouse liver tissue lysate in the 1) absence and 2) presence of immunizing peptide using HES5 antibody at 7 ug/ml. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HES5-Antibody-Western-Blot-NBP2-27174-img0002.jpg "Western Blot: HES5 Antibody [NBP2-27174] - analysis of HES5 in mouse liver tissue lysate in the 1) absence and 2) presence of immunizing peptide using HES5 antibody at 7 ug/ml. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: HES5 Antibody [NBP2-27174] - analysis of HES5 in formalin-fixed, paraffin-embedded human brain tissue using HES5 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/HES5-Antibody-Immunohistochemistry-Paraffin-NBP2-27174-img0001.jpg)
![Western Blot: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to GAPDH protein, detected mainly in the cytosolic fractions of these cells.](http://images.novusbio.com/fullsize/SF3B4-Antibody-3A1-Western-Blot-NBP1-92692-img0005.jpg "Western Blot: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to GAPDH protein, detected mainly in the cytosolic fractions of these cells.")
![Immunocytochemistry/Immunofluorescence: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of HeLa cells stained with mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The NBP1-92692 antibody reveals strong granular staining of the nuclei, while the vimentin antibody specifically labels cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/SF3B4-Antibody-3A1-Immunocytochemistry-Immunofluorescence-NBP1-92692-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SF3B4 Antibody (3A1) [NBP1-92692] - HeLa cells stained with NBP1-92692 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (blue, stained with DAPI). The SF3B4 (3A1) antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The Vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.](http://images.novusbio.com/fullsize/SF3B4-Antibody-3A1-Immunocytochemistry-Immunofluorescence-NBP1-92692-img0003.jpg)