| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81817-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08936 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87753-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77179-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70854 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72642 | |||
![Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Western-Blot-NBP2-34621-img0002.jpg "Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34621-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34621-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0005.jpg)
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining in human cerebral cortex and skeletal muscle tissues using anti-MAG antibody. Corresponding MAG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP1-81817-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining in human cerebral cortex and skeletal muscle tissues using anti-MAG antibody. Corresponding MAG RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP1-81817-img0006.jpg)
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - MAG Antibody [NBP1-81817] - Image submitted by a verified customer review on mouse inner ear at 1:200 dilution.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-NBP1-81817-img0004.jpg)
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP1-81817-img0005.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Analysis in human cerebral cortex and cervix, uterine tissues using NBP2-33774 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-33774_rabbit-polyclonal-gfap-antibody-immunohistochemistry-paraffin-166202374820.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Analysis in human cerebral cortex and cervix, uterine tissues using NBP2-33774 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GFAP Antibody [NBP2-33774] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NBP2-33774-img0043.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NBP2-33774] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33774-img0017.jpg)
![Western Blot: GFAP Antibody [NBP2-33774] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NBP2-33774-img0016.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] - Staining of human hippocampus shows positivity in astrocytes.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-33774-img0039.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] - Staining of human pancreas shows no positivity as expected.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-33774-img0040.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Staining of human cervix, uterine shows no cytoplasmic positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-33774_rabbit-polyclonal-gfap-antibody-immunohistochemistry-paraffin-166202375644..jpg)
![Immunocytochemistry/Immunofluorescence: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-212024940874.jpg "Immunocytochemistry/Immunofluorescence: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249371512.jpg)
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249432830.jpg)
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Whole cell extract (30 Aug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249371526.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249425457.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249362765.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-212024940889.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3R1 antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-western-blot-141120251844343.jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025163536..jpg "<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-294202516917..jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025161213..jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2802) [NBP3-45161] - Tau antibody [HL2802] detects Tau protein at cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2802] diluted at 1:250.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2942025161556..jpg)
![Immunohistochemistry: Rabbit Tau mAb (HL2802) [NBP3-45161] - Tau antibody [HL2802] detects Tau protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Tau stained by Tau antibody [HL2802] diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunohistochemistry-paraffin-2942025161950..jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025162224..jpg)
![Tau antibody [HL2802] detects Tau protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: Tau stained by Tau antibody [HL2802] (NBP3-45161) diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32618_rabbit-polyclonal-protein-phosphatase-1-beta-antibody-immunohistochemistry-frozen-141120251855420.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0005.jpg "Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0003.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human A375 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human melanoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Analysis in human cerebral cortex and endometrium tissues using NBP1-87753 antibody. Corresponding NEFM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Analysis in human cerebral cortex and endometrium tissues using NBP1-87753 antibody. Corresponding NEFM RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cell.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-NBP1-87753-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NF-M Antibody [NBP1-87753] - Analysis NBP1-87753 (A) shows similar pattern to independent antibody NBP1-87754 (B).](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87753] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87753-img0011.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0018.jpg)
![Western Blot: NF-M Antibody [NBP1-87753] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: NF-M Antibody [NBP1-87753] - Analysis in human cell line HEK 293 and human cell line A-431.](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87753] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87753-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebral cortex shows strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0015.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human prostate shows strong positivity in peripheral nerve.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0017.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0019.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518341523.jpg "Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded rat esophagus.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-1411202518382820.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518435619.jpg)
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-141120251841520.jpg)
![AKT antibody [HL2915] detects AKT protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunocytochemistry-immunofluorescence-1411202518424111.jpg)
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-1411202518312110.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518394118.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518334523.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Analysis in EL4 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.Red: ZEB2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202419333477.gif)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, and then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0012.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - The protein levels of EMT-TFs were compared between carcinoma cell lines and non- or less-malignant tumor cell lines by using Western-blot analysis. The soft agar colony transformation or migration assay was referred to distinguish malignant degree of the experimental cancer cell lines (data not shown). Malignant degree: prostate cancer cell lines, P69 < M12; breast cancer cell lines, MCF7 < MD-MBA-231; lung cancer cell lines, A549 < H1299; liver cancer cell lines, HepG2 < SMMC7721. 'kD' (kilo-dalton) means the molecular weight of protein. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0013.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415392525.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415334920.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415395947.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241535644.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Functional domains of Fbxo45 for ubiquitination of Zeb2A. Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, & then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. B. Bacterially expressed GST or GST–Fbxo45, -Fbxo45 delta F-box, -Fbxo45 delta mid, or -Fbxo45 delta SPRY fusion proteins & Glutathione-Sepharose beads were incubated with lysates of HeLa cells transfected with Zeb2, Snai1 or Snai2, respectively. The proteins associated with GST–tagged Fbxo45 forms, bound on the Glutathione-Sepharose beads were washed five times with the RIPA buffer before immunoblotting. C. Lysates from 293T cells transfected Flag-Zeb2 with HA-Fbxo45, -Fbxo45 delta mid, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were immunoprecipitated with anti-Flag or anti-HA antibodies, & immunoprecipitates were resolved by SDS-PAGE for Western-blot analysis. D. U2OS cells transfected with Flag-Zeb2 & HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were stained using the primary antibodies of anti-Flag M2 or anti-HA, & the second antibodies of Alexa Fluor 568 anti-mouse or Alexa Fluor 488 anti-Rabbit, respectively. Scale: 25μm. E-I. Zeb2 ubiquitination assays by using IP expriments under different conditions: Flag-Zeb2 with or without HA-Ub (E); Flag-Zeb2 with HA-Ub WT, K48R or K63R (F); Flag-Zeb2 & HA-Ub K48-only with or without myc-Fbox45 (G); Flag-Zeb2 & HA-Ub K48-only with siRNA control, siRNAs for Fbxo45 or siRNAs for Pam (H); Flag-Zeb2 & HA-Ub with HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY (I). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416163752.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416175234.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241616011.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420285893.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420272496.jpg)
![Western Blot: NMDAR2B Antibody (NR2B) [NB100-74475] - Analysis of NMDA receptor type 2B in mouse brain tissue.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-NR2B-Western-Blot-NB100-74475-img0003.jpg "Western Blot: NMDAR2B Antibody (NR2B) [NB100-74475] - Analysis of NMDA receptor type 2B in mouse brain tissue.")
![Immunohistochemistry-Paraffin: NMDAR2B Antibody (NR2B) [NB100-74475] - Analysis showing staining in the cytoplasm and membrane of human breast tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-NR2B-Immunohistochemistry-Paraffin-NB100-74475-img0004.jpg)
![Western Blot: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutathione Synthetase.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Western-Blot-NBP2-03351-img0002.jpg "Western Blot: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutathione Synthetase.")
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] Staining of paraffin-embedded Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03351-img0005.jpg)
![Flow Cytometry: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Glutathione Synthetase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Flow-Cytometry-NBP2-03351-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03351-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - Staining of paraffin-embedded Human Kidney tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03351-img0004.jpg)
![Western Blot: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Meis homeobox 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Meis homeobox 3.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Western-Blot-NBP2-03933-img0015.jpg "Western Blot: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Meis homeobox 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Meis homeobox 3.")
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Human tonsil using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0014.jpg)
![Flow Cytometry: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Meis homeobox 3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Flow-Cytometry-NBP2-03933-img0016.jpg)
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0009.jpg)
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0010.jpg)
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Human Kidney tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0011.jpg)
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Human pancreas tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0012.jpg)
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Human prostate tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0013.jpg)