| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38856-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61764-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96431-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34413-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-126SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59668SS | |||
| Azide and BSA Free | NBP2-80958 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19344SS | |||
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining in human heart muscle and skin tissues.. Corresponding CDH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining in human heart muscle and skin tissues.. Corresponding CDH2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody [NBP2-38856] - Disruption of N-cadherin shortens burst duration without affecting other calcium spike parameters. Immunohistochemistry showing N-cadherin (red, upper left), K-cadherin (red, lower left) and F-actin (green, middle) are abundant in the zG layer (right, merged images). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15408-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38856-img0010.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0009.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0006.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human skin shows no positivity in keratinocytes.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0007.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0008.jpg)
![Western Blot: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of 75 ug of various whole cell lysates and 10 ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Western-Blot-NBP2-22481-img0001.jpg "Western Blot: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of 75 ug of various whole cell lysates and 10 ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 in NCCIT and HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a LIN28 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0005.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the cytoplasm of human seminoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunohistochemistry-Paraffin-NBP2-22481-img0015.jpg)
![Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on H9 embryonic stem cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Flow-Cytometry-NBP2-22481-img0013.jpg)
![Chromatin Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis performed using cross-linked chromatin from rat hepatoma cells treated with insulin. IP performed using a multiplex microplate Matrix ChIP assay of LIN28 monoclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2-3 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the rat Egr-1 locus is shown; oxes represent exons (black boxes = translated, white boxes = untranslated), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Chromatin-Immunoprecipitation-ChIP-NBP2-22481-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0004.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the nucleus and cytoplasm of mouse testis tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunohistochemistry-Paraffin-NBP2-22481-img0014.jpg)
![Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on HEL 11.4 induced IPS cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Flow-Cytometry-NBP2-22481-img0012.jpg)
![Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 was performed. Antigen-antibody complexes were formed by incubating 700ug of lysate with 5 ug of an LIN28 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Agarose was loaded as a positive control for detection. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a LIN28 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunoprecipitation-NBP2-22481-img0002.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0008.jpg "Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of esophageal cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0006.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0002.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0007.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0003.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of breast cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0005.jpg)
![ELISA: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0001.jpg)
![Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Knockout-Validated-NB110-96431-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Load: 15 ug protein. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Western-Blot-NB110-96431-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (8A7) [NB110-96431] - analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Courtesy of: Mike Welsh, Umich.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunocytochemistry-Immunofluorescence-NB110-96431-img0007.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0016.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (8A7) [NB110-96431] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-Paraffin-NB110-96431-img0008.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0017.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NBP1-31258-img0009.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.")
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NBP1-31258-img0008.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-91020242001028.jpg)
![Immunocytochemistry/Immunofluorescence: GPER/GPR30 Antibody [NBP1-31258] - DIV11 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-16122024114732..png)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Mouse tissues.GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunohistochemistry-paraffin-1612202411499..png)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled AsPC-1 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518352213.jpg)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518344912.jpg)
![GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-1411202518434418.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518344921.jpg)
![GPER/GPR30 antibody detects GPER/GPR30 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPER/GPR30 stained by GPER/GPR30 antibody (NBP1-31258) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-141120251841523.jpg)
![Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM507-Western-Blot-NBP2-34365-img0004.jpg "Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).")
![Immunohistochemistry-Paraffin: GFAP Antibody (SPM507) [NBP2-34365] - Formalin-paraffin human brain stained with GFAP Ab (SPM507). Note cytoplasmic staining.](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM507-Immunohistochemistry-Paraffin-NBP2-34365-img0001.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. CRISPR knockout cell lines generated by Edigene](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-30883] - Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. CRISPR knockout cell lines generated by Edigene")
![<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-30883] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (NBP1-30883). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-30883] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 protein stained by Stathmin 1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30883-img0007.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:H129912% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0003.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE Stathmin 1 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0009.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE Stathmin 1 antibody dilution: 1:2000](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-30883] - Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 protein stained by Stathmin 1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30883-img0006.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-30883] - Sample: Paraffin-embedded human breast carcinoma. Stathmin 1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30883-img0005.jpg)
![Western Blot: CLOCK Antibody [NB100-126] - Lanes 3-5 contained alpha CLOCK immunoprecipitated mouse DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay.](http://images.novusbio.com/fullsize/CLOCK-Antibody-Western-Blot-NB100-126-img0002.jpg "Western Blot: CLOCK Antibody [NB100-126] - Lanes 3-5 contained alpha CLOCK immunoprecipitated mouse DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay.")
![Western Blot: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - Total protein from Human and Mouse Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Spectrin Beta III in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Western-Blot-NBP2-59668-img0004.jpg "Western Blot: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - Total protein from Human and Mouse Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Spectrin Beta III in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Spectrin beta 3 (1287A) at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Immunocytochemistry-Immunofluorescence-NBP2-59668-img0002.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human brain using Spectrin beta III antibody at a 1:1,000 dilution. The primary antibody bound to Spectrin beta III was detected with HRP-DAB detection method and the nuclei were counterstained with hematoxylin. This Spectrin beta III antibody generated a specific cytoplasmic staining in all the cells with strongest signal in neuronal cells.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Immunohistochemistry-Paraffin-NBP2-59668-img0005.jpg)
![Flow (Intracellular): Spectrin beta 3 Antibody (1287A) [NBP2-59668] - An intracellular stain was performed on HeLa Cells with Spectrin beta 3 (1287A) antibody NBP2-59668 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Flow-Intracellular-NBP2-59668-img0001.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser276] Antibody-Western Blot-NBP1-77807-img0010.jpg "Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.")
![Immunohistochemistry: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - Diluted to1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-NBP1-77807-img0007.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS529 shows phospho p65 staining in carcinoma cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0005.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0006.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0008.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0020.jpg "Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Mouse brain. AChE stained by AChE antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0021.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0009.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0014.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Paraformaldehyde-fixed HeLa, using AChE antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31329-img0008.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Mouse colon. AChE antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0010.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat fore brain. AChE antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0011.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat brain. AChE antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0012.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-91020242091450.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-910202420104428.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-91020242001048.jpg)
![Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein by immunofluorescent analysis.Sample: DIV9 rat hippocampal neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-immunocytochemistry-immunofluorescence-1411202518443420.jpg)
![Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Acetylcholinesterase/ACHE protein stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48812_rabbit-opsin-1-medium-wave-mab-hl3435-immunohistochemistry-frozen-14112025185548.jpg)
![Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Western-Blot-NBP1-19344-img0005.jpg "Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.")
![Immunohistochemistry: Profilin 1 Antibody [NBP1-19344] - Staining of Profilin in mouse thymus.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Immunohistochemistry-NBP1-19344-img0004.jpg)
![Simple Western: Profilin 1 Antibody [NBP1-19344] - Simple Western lane view shows a specific band for Profilin 1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Simple-Western-NBP1-19344-img0006.jpg)