| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33060 | |||
| IHC-Prediluted | NBP2-44830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78964SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22450-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47950-0.1mg | |||
| IHC-Prediluted | NBP2-48191 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74344 | |||
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0007.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Protein-Array-NBP2-29433-img0006.jpg)
![Western Blot: BIRC6 Antibody [NB110-40730] - Dual IAP-targeting ASOs knockdown BIRC6, cIAP1 or survivin proteins and lead to marked suppression of CRPC cell proliferation. Western blotting showing protein levels of BIRC6, cIAP1 and survivin in two CRPC cell lines (A) PC-3 cells and (B) C4-2 cells transfected with Mock or increasing dosages of scrambled ASO (Scrb), dASOs 6w2 and 6w5 for 72 hr. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Western-Blot-NB110-40730-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: BIRC6 Antibody [NB110-40730] - Dual IAP-targeting ASOs knockdown BIRC6, cIAP1 or survivin proteins and lead to marked suppression of CRPC cell proliferation. Western blotting showing protein levels of BIRC6, cIAP1 and survivin in two CRPC cell lines (A) PC-3 cells and (B) C4-2 cells transfected with Mock or increasing dosages of scrambled ASO (Scrb), dASOs 6w2 and 6w5 for 72 hr. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BIRC6 Antibody [NB110-40730] - Treatments with dASOs resulted in significant lower viable tumor volume. Representative images of control ASO-6w2- and 6w5-treated PC-3 xenografts using H&E, and IHC staining of BIRC6, cleaved-caspase 3 and Ki-67. 20x magnification. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-NB110-40730-img0005.jpg)
![Immunohistochemistry-Paraffin: BIRC6 Antibody [NB110-40730] - FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbitanti-BIRC6/Apollon used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB110-40730-img0003.jpg)
![Immunohistochemistry-Paraffin: BIRC6 Antibody [NB110-40730] - FFPE section of human ovarian tumor. Antibody: Affinity purified rabbit antiBIRC6/Apollon used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB110-40730-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BIRC6 Antibody [NB110-40730] - Elevated BIRC6 expression is associated with advanced stages of prostate cancer: co-upregulation of other IAP members. Representative images of correlated expressions between BIRC6 and survivin, XIAP and cIAP1. 20x magnification, scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-NB110-40730-img0004.jpg)
![Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP1-78964-img0015.jpg "Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.")
![Immunocytochemistry/Immunofluorescence: LC3A Antibody [NBP1-78964] - IF Confocal analysis of A549 cells using LC3I antibody (NBP1-78964, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78964-img0017.jpg)
![Immunohistochemistry: LC3A Antibody [NBP1-78964] - Analysis of LC3I in mouse testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-NBP1-78964-img0016.jpg)
![Western Blot: HCN1 Antibody (S70) [NBP1-22450] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Western-Blot-NBP1-22450-img0011.jpg "Western Blot: HCN1 Antibody (S70) [NBP1-22450] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Neurons.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0014.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Human Hippocampus](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0001.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0012.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0013.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0015.jpg)
![Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.Positive control:Lane 1: 293 Lane 2: MCF-7Lane 3: SK-Br-3 Lane 4: Mouse kidney](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Western-Blot-NBP2-75440-img0009.jpg "Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.Positive control:Lane 1: 293 Lane 2: MCF-7Lane 3: SK-Br-3 Lane 4: Mouse kidney")
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0004.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0008.jpg)
![Flow Cytometry: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Flow-Cytometry-NBP2-75440-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0003.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0005.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0006.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0007.jpg)
![Western Blot: Vimentin Antibody (VM1170) [NBP2-44833] - Western Blot Analysis of HEP2 cell lysate using Vimentin Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Western-Blot-NBP2-44833-img0008.jpg "Western Blot: Vimentin Antibody (VM1170) [NBP2-44833] - Western Blot Analysis of HEP2 cell lysate using Vimentin Antibody (VM1170).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Vimentin Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Angiosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Ewing's Sarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Leiomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Melanoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Rhabdomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Uterus stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM1170-Immunohistochemistry-Paraffin-NBP2-44833-img0006.jpg)
![Western Blot: SPR Antibody (4F5) [NBP2-03256] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Western-Blot-NBP2-03256-img0013.jpg "Western Blot: SPR Antibody (4F5) [NBP2-03256] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SPR Antibody (4F5) [NBP2-03256] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunocytochemistry-Immunofluorescence-NBP2-03256-img0012.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human pancreas tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0011.jpg)
![Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPR antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Flow-Cytometry-NBP2-03256-img0002.jpg)
![Western Blot: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Western-Blot-NBP2-03256-img0003.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0004.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0005.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0006.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0007.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0008.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human Kidney tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0009.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human liver tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0010.jpg)
![Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - Analysis of Jurkat cells, using anti-SPR antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Flow-Cytometry-NBP2-03256-img0001.jpg)
![Western Blot: NPY1R Antibody [NBP1-59008] - Human Fetal Brain Lysate, concentration 1ug/ml.](http://images.novusbio.com/fullsize/NPY1R-Antibody-Western-Blot-NBP1-59008-img0010.jpg "Western Blot: NPY1R Antibody [NBP1-59008] - Human Fetal Brain Lysate, concentration 1ug/ml.")
![Immunohistochemistry: NPY1R Antibody [NBP1-59008] - Analysis of mouse gut tissue.](http://images.novusbio.com/fullsize/NPY1R-Antibody-Immunohistochemistry-NBP1-59008-img0011.jpg)
![Immunohistochemistry-Paraffin: NPY1R Antibody [NBP1-59008] - Human liver.](http://images.novusbio.com/fullsize/NPY1R-Antibody-Immunohistochemistry-Paraffin-NBP1-59008-img0007.jpg)
![Immunohistochemistry-Paraffin: NPY1R Antibody [NBP1-59008] - Human Testis Tissue, 5ug/ml](http://images.novusbio.com/fullsize/NPY1R-Antibody-Immunohistochemistry-Paraffin-NBP1-59008-img0008.jpg)
![Immunohistochemistry-Paraffin: NPY1R Antibody [NBP1-59008] - Human Gut Tissue, primary dilution 10ug/ml and 2ug/ml. Secondary goat anti-rabbit Cy3 at 1:500.](http://images.novusbio.com/fullsize/NPY1R-Antibody-Immunohistochemistry-Paraffin-NBP1-59008-img0009.jpg)
![Western Blot: NPY1R Antibody [NBP1-59008] - Representative immunoblotting images showing the protein expression of NPY1R in (E) MCF7, (F) T47D and (G) ZR75-1 parental cells and their EDR and TamR derivatives. Ponceau staining was used to assess equal protein loading for visual assessment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35121782/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-59008_rabbit-polyclonal-npy1r-antibody-25520231539514.jpg)
![Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Western-Blot-NBP2-74344-img0002.jpg "Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.")
![Immunocytochemistry/Immunofluorescence: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74344-img0005.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human liver tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0012.jpg)
![Flow Cytometry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SPR antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74344-img0004.jpg)
![Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Western-Blot-NBP2-74344-img0001.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0006.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0007.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0008.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0009.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0010.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human Kidney tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0011.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human pancreas tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0013.jpg)
![Flow Cytometry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Analysis of Jurkat cells, using anti-SPR antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74344-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0012.jpg "Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31555-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Rat duodenum. NAGLU antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse brain. NAGLU antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0011.jpg)
![alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) .Blue: Nuclear staining with Hoechst 33342. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31555_rabbit-polyclonal-alpha-n-acetylglucosaminidase-naglu-antibody-immunohistochemistry-paraffin-141120251843564.jpg)
![alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha-N-acetylglucosaminidase/NAGLU stained by alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp1-32244_rabbit-polyclonal-calretinin-antibody-immunohistochemistry-frozen-141120251855418.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Western-Blot-NBP2-19952-img0004.jpg "Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP2-19952] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19952-img0002.jpg)
![Western Blot: Proteasome 19S S5A Antibody [NBP2-19952] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-19952-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 was knocked down in HCT116 cells by four different siRNAs and the cell lysates immunoprobed as indicated. Mock and scrambled control samples are included. b-actin is used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Knockdown-Validated-NBP2-19952-img0003.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241537198.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241538418.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202415291972.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113548.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113543.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241611358.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416121996.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - A C-terminal domain in hRpn10 binds E6AP AZUL.a Positions of known functional domains within hRpn10 (top) & E6AP isoform II (bottom). Question mark “?” indicates hRpn10 uncharacterized region & the E6AP catalytic cysteine C843 is indicated. b Pull-down assay of His-tagged hRpn10full-length (full), hRpn10196–377 or hRpn10196–306 without (−) or with (+) incubation of E6AP. c1H, 15N HSQC spectra of 0.2 mM 15N-hRpn10305–377 (black) & with twofold molar excess unlabeled AZUL (green). Shifted signals are labeled. d Table summarizing Kd, kon, & koff average values with standard deviations for the hRpn10305–377: AZUL interaction measured by ITC and/or SPR. N/A, not applicable. e HCT116 lysates expressing empty vector, myc-hRpn10 full length, or myc-Rpn10 with RAZUL deleted ( delta 307–377) were subjected to myc-immunoprecipitation with anti-myc-tag nanobody-coupled agarose. Whole cell extracts (WCE) & myc-immunoprecipitates were immunoprobed with the indicated antibodies. Cyclophilin B (Cyp B) is used as a loading control in e & f. f Lysates from HCT116 cells expressing HA-E6AP & the myc-hRpn10 constructs of e were subjected to HA IP followed by immunoblotting with the indicated antibodies. An asterisk “*” indicates non-specific interaction; double asterisk “**” indicates heavy chain antibody. e–f All antibodies used for immunoprobing are indicated to the left of the images. Note that the hRpn10 & E6AP antibodies recognize both endogenous & exogenously expressed protein, causing these panels to show both tagged & endogenous protein. g Schematic representation highlighting interaction domains of hRpn10 including newly identified RAZUL. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416114714.jpg)