| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93073-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59325-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59335-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61659-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44249SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58360SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50032SS | |||
![Western Blot: PDCD10 Antibody - Azide and BSA Free [NBP2-93073] - Western blot analysis of various lysates, using PDCD10 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp2-93073_rabbit-polyclonal-pdcd10-antibody-4220251430419.jpg "Western Blot: PDCD10 Antibody - Azide and BSA Free [NBP2-93073] - Western blot analysis of various lysates, using PDCD10 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.")
![Immunohistochemistry-Paraffin: PDCD10 Antibody [NBP2-93073] - Paraffin-embedded rat spleen using PDCD10 .](http://images.novusbio.com/fullsize/PDCD10-Antibody-Immunohistochemistry-Paraffin-NBP2-93073-img0007.jpg)
![Immunohistochemistry-Paraffin: PDCD10 Antibody [NBP2-93073] - Paraffin-embedded human breast cancer using PDCD10 .](http://images.novusbio.com/fullsize/PDCD10-Antibody-Immunohistochemistry-Paraffin-NBP2-93073-img0002.jpg)
![Immunohistochemistry-Paraffin: PDCD10 Antibody [NBP2-93073] - Paraffin-embedded mouse kidney using PDCD10 .](http://images.novusbio.com/fullsize/PDCD10-Antibody-Immunohistochemistry-Paraffin-NBP2-93073-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: PDCD10 Antibody - Azide and BSA Free [NBP2-93073] - Immunofluorescence analysis of PC-12 cells using PDCD10 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93073_rabbit-polyclonal-pdcd10-antibody-42202514264480.jpg)
![Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mouse mAb against PC-12 (1), LNCAP (2) and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Western-Blot-NBP2-37309-img0004.jpg "Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mouse mAb against PC-12 (1), LNCAP (2) and NIH/3T3 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis of Hela cells using DNM1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Immunocytochemistry-Immunofluorescence-NBP2-37309-img0005.jpg)
![Flow Cytometry: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis of HEK293 cells using DNM1L mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Flow-Cytometry-NBP2-37309-img0006.jpg)
![Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mAb against human DNM1L recombinant protein. (Expected MW is 41.8 kDa)](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Western-Blot-NBP2-37309-img0002.jpg)
![Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mAb against HEK293 (1) and DNM1L (AA: 69-213)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Western-Blot-NBP2-37309-img0003.jpg)
![ELISA: DRP1 Antibody (4A4C7) [NBP2-37309] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-ELISA-NBP2-37309-img0001.jpg)
![Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA-AR alpha 2 Alpha 2 protein using Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 ug. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-2-Antibody-S399-19-Western-Blot-NBP2-59325-img0008.jpg "Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA-AR alpha 2 Alpha 2 protein using Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 ug. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-2-Antibody-S399-19-Immunocytochemistry-Immunofluorescence-NBP2-59325-img0009.jpg)
![Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA-AR alpha 2 Alpha 2 protein using Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa. Other Band(s): ~ 37 kDa, ~50 kDa, ~70 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-2-Antibody-S399-19-Western-Blot-NBP2-59325-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/GABA-AR-alpha-2-Antibody-S399-19-Immunocytochemistry-Immunofluorescence-NBP2-59325-img0002.jpg)
![Western Blot: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA-B R2 protein using Mouse Anti-GABA-B R2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-GABA-B R2 Monoclonal Antibody (NBP2-59335) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Western-Blot-NBP2-59335-img0005.jpg "Western Blot: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA-B R2 protein using Mouse Anti-GABA-B R2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-GABA-B R2 Monoclonal Antibody (NBP2-59335) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody (NBP2-59335) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Immunocytochemistry-Immunofluorescence-NBP2-59335-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Immunocytochemistry-Immunofluorescence-NBP2-59335-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody (NBP2-59335) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-B Receptor 2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Immunocytochemistry-Immunofluorescence-NBP2-59335-img0007.jpg)
![Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Western-Blot-NBP2-61659-img0004.jpg "Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Immunocytochemistry-Immunofluorescence-NBP2-61659-img0003.jpg)
![Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Raji cells using NF-M mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Flow-Cytometry-NBP2-61659-img0007.jpg)
![Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 779-916)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Western-Blot-NBP2-61659-img0005.jpg)
![Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Flow-Cytometry-NBP2-61659-img0002.jpg)
![ELISA: NF-M Antibody (1B6B12) [NBP2-61659] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-ELISA-NBP2-61659-img0001.jpg)
![ELISA: NF-M Antibody (1B6B12) [NBP2-61659] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-ELISA-NBP2-61659-img0006.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-28820241423313.jpg "Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29820241435223.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29202410484898.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-39202488464.jpg)
![Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-288202412461658.jpg "Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32834) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-298202414333688.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunocytochemistry analysis of HepG2 cells labeling PMP70 with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-29202410334056.jpg)
![Flow Cytometry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Flow cytometric analysis of NIH/3T3 cells labeling PMP70. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32834, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-2920241637212.jpg)
![Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-26820241525104.jpg "Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: SOX2 Antibody (PO00-28) [NBP3-32972] - Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-28820241249344.jpg)
![Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-298202414161844.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-29202410334048.jpg)
![Western Blot: Filamin A Antibody [NB100-58812] - Whole cell lysate (5, 15 and 50 ug for WB) from HeLa cells. Antibody used for WB at 0.04 ug/ml.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Western-Blot-NB100-58812-img0003.jpg "Western Blot: Filamin A Antibody [NB100-58812] - Whole cell lysate (5, 15 and 50 ug for WB) from HeLa cells. Antibody used for WB at 0.04 ug/ml.")
![Immunohistochemistry-Paraffin: Filamin A Antibody [NB100-58812] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Filamin A used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NB100-58812-img0005.jpg)
![Western Blot: CD133 Antibody [NBP2-44249] - Total mouse placenta protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-mCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD133-Antibody-Western-Blot-NBP2-44249-img0001.jpg "Western Blot: CD133 Antibody [NBP2-44249] - Total mouse placenta protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-mCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD133 Antibody [NBP2-44249] - NIH/3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-mCD133 (M30) (NBP2-44249) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44249-img0002.jpg)
![Western Blot: FE65 Antibody [NB110-58360] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY APBB1 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APBB1.](http://images.novusbio.com/fullsize/FE65-Antibody-Western-Blot-NB110-58360-img0006.jpg "Western Blot: FE65 Antibody [NB110-58360] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY APBB1 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APBB1.")
![Western Blot: FE65 Antibody [NB110-58360] - Protein on HEK 293 cell lysate.](http://images.novusbio.com/fullsize/FE65-Antibody-Western-Blot-NB110-58360-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Mixed neuron and glia cultures stained with NBP2-50032 (red) and chicken antibody to Vimentin (green) and DNA (blue). NBP2-50032 reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while Vimentin was visualized in astrocytes and fibroblasts.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Immunocytochemistry-Immunofluorescence-NBP2-50032-img0004.jpg "Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Mixed neuron and glia cultures stained with NBP2-50032 (red) and chicken antibody to Vimentin (green) and DNA (blue). NBP2-50032 reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while Vimentin was visualized in astrocytes and fibroblasts.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Analysis of adult rat hippocampus section stained with mouse mAb to MAP2A/B/C/D, NBP2-50032, dilution 1:5,000 in green, and chicken pAb to FOX2, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. NBP2-50032 antibody labels all isotypes of the MAP2 protein which are found in the perikarya and dendrites of neurons. The FOX2 antibody stains the nuclei of a subset of neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Immunocytochemistry-Immunofluorescence-NBP2-50032-img0006.jpg)
![Western Blot: MAP2 Antibody (2C4) [NBP2-50032] - Analysis of tissue and cell lysates using mouse mAb to MAP2C/D, NBP2-50032, dilution 1:5,000 in green, and chicken pAb to Vimentin, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH/3T3 cell lysates. A band at about 280 kDa corresponds to full length intact MAP2A/2B isotypes. The bands that appear at about 70kDa represent MAP2C/D isotypes. Multiple bands in between are likely to be in vivo fragments of MAP2A/B. Only the SH-SY5Y cells, which have neuronal properties express MAP2 protein. Vimentin antibody binds to vimentin protein showing a single band at ~50 kDa, which is present in all preparations.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Western-Blot-NBP2-50032-img0005.jpg)
