| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16924-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93840-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94362-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-75290-0.025mg | |||
![Immunoprecipitation:Striatin AntibodyNBP3-29144] -Detection of human Striatin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Striatin antibody NBP3-29144 (lot NBP3-29144-1) used for IP at 6 µg per reaction. Striatin was also immunoprecipitated by rabbit anti-Striatin antibody BL17187. For blotting immunoprecipitated Striatin, NBP3-29144 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29144_rabbit-striatin-pab-3012202416502212.jpg "Immunoprecipitation:Striatin AntibodyNBP3-29144] -Detection of human Striatin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Striatin antibody NBP3-29144 (lot NBP3-29144-1) used for IP at 6 µg per reaction. Striatin was also immunoprecipitated by rabbit anti-Striatin antibody BL17187. For blotting immunoprecipitated Striatin, NBP3-29144 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:HIP1 Related AntibodyNBP3-30685] -Detection of human HIP1R/HIP12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HIP1R/HIP12 antibody NBP3-30685 (lot NBP3-30685-1) used for IP at 6 µg per reaction. HIP1R/HIP12 was also immunoprecipitated by rabbit anti-HIP1R/HIP12.. For blotting immunoprecipitated HIP1R/HIP12, NBP3-30685 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30685_rabbit-hip1-related-pab-301220241332429.jpg "Immunoprecipitation:HIP1 Related AntibodyNBP3-30685] -Detection of human HIP1R/HIP12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HIP1R/HIP12 antibody NBP3-30685 (lot NBP3-30685-1) used for IP at 6 µg per reaction. HIP1R/HIP12 was also immunoprecipitated by rabbit anti-HIP1R/HIP12.. For blotting immunoprecipitated HIP1R/HIP12, NBP3-30685 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: GRK2 Antibody [NB100-2831] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence. Approx 80 kDa band (calculated MW of 79.7 kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48 kDa and was successfully blocked by incubation with the immunizing peptide.](http://images.novusbio.com/fullsize/GRK2-Antibody-Western-Blot-NB100-2831-img0001.jpg "Western Blot: GRK2 Antibody [NB100-2831] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence. Approx 80 kDa band (calculated MW of 79.7 kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48 kDa and was successfully blocked by incubation with the immunizing peptide.")
![Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/Tau-[p-Ser202-p-Ser199-p-Ser198]-Antibody-Western-Blot-NBP3-16924-img0001.jpg "Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.")
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP3-05429] - Western blot analysis of extracts from mouse brain tissue, using alpha-Synuclein antibody (NBP3-05429). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP3-05429-img0001.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP3-05429] - Western blot analysis of extracts from mouse brain tissue, using alpha-Synuclein antibody (NBP3-05429). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: PPP2R2A Antibody (9E5N2) [NBP3-33476] - Western blot analysis of lysates from HeLa cells, using PPP2R2A Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33476_rabbit-ppp2r2a-mab-9e5n2-912202415161827.jpg "Western Blot: PPP2R2A Antibody (9E5N2) [NBP3-33476] - Western blot analysis of lysates from HeLa cells, using PPP2R2A Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: PPT1 Antibody [NBP2-93840] - Analysis of extracts of various cell lines, using PPT1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/PPT1-Antibody-Western-Blot-NBP2-93840-img0001.jpg "Western Blot: PPT1 Antibody [NBP2-93840] - Analysis of extracts of various cell lines, using PPT1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Western Blot: Myt1 Antibody [NBP2-94362] - Analysis of extracts of various cell lines, using Myt1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/Myt1-Antibody-Western-Blot-NBP2-94362-img0001.jpg "Western Blot: Myt1 Antibody [NBP2-94362] - Analysis of extracts of various cell lines, using Myt1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.")
![Western Blot: Presenilin-1 Antibody (3W4V8) [NBP3-15665] - Western blot analysis of extracts of various cell lines, using Presenilin-1 antibody (NBP3-15665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-3W4V8-Western-Blot-NBP3-15665-img0001.jpg "Western Blot: Presenilin-1 Antibody (3W4V8) [NBP3-15665] - Western blot analysis of extracts of various cell lines, using Presenilin-1 antibody (NBP3-15665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Western Blot: TDO2 Antibody (OTI1D1) - Azide and BSA Free [NBP2-74485] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDO2.](http://images.novusbio.com/fullsize/TDO2-Antibody-OTI1D1-Azide-and-BSA-Free-Western-Blot-NBP2-74485-img0001.jpg "Western Blot: TDO2 Antibody (OTI1D1) - Azide and BSA Free [NBP2-74485] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDO2.")
![Western Blot: Tau [p Ser199] Antibody (JE46-95) [NBP3-32803] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32803) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Human brain tissue lysate, the membrane treated with pp for 1 hour Lane 5: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lane 6: Rat brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-70 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32803) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32803_rabbit-tau-p-ser199-mab-je46-95-288202412461637.jpg "Western Blot: Tau [p Ser199] Antibody (JE46-95) [NBP3-32803] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32803) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Human brain tissue lysate, the membrane treated with pp for 1 hour Lane 5: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lane 6: Rat brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-70 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32803) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")