| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47911-0.1mg | |||
| IHC-Prediluted | NBP2-48363 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48226 | |||
| Azide and BSA Free | NBP2-34705-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-46853SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-59909SS | |||
![Western Blot: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19460-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: N Cadherin Antibody [NBP2-19460] - Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19460-img0002.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19460] - Paraffin-embedded hepatoma. N-Cadherin antibody [N2C1], Internal dilution: 1:500.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19460-img0003.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19460] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-91020242014643.jpg)
![N-Cadherin antibody [N2C1], Internal detects N-Cadherin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-immunohistochemistry-frozen-261120251842318.jpg)
![Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251828100.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251846100.jpg)
![Western Blot: WWOX Antibody [NBP1-33456] - Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with WWOX antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP1-33456-img0007.jpg "Western Blot: WWOX Antibody [NBP1-33456] - Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with WWOX antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP1-33456] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33456-img0003.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456] - WWOX antibody detects WWOX protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. WWOX antibody dilution: 1:500.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP1-33456-img0009.jpg)
![Western Blot: WWOX Antibody [NBP1-33456] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP1-33456-img0004.jpg)
![Western Blot: WWOX Antibody [NBP1-33456] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP1-33456-img0005.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456] - WWOX antibody detects WWOX protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. WWOX antibody dilution: 1:100.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP1-33456-img0008.jpg)
![Immunoprecipitation: WWOX Antibody [NBP1-33456] - WWOX antibody immunoprecipitates WWOX protein in IP experiments. IP samples: 293T whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of WWOX protein by 4 µg WWOX antibody. 10 % SDS-PAGE. The immunoprecipitated WWOX protein was detected by WWOX antibody diluted at 1:500.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunoprecipitation-NBP1-33456-img0006.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Western-Blot-NBP2-19952-img0004.jpg "Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP2-19952] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19952-img0002.jpg)
![Western Blot: Proteasome 19S S5A Antibody [NBP2-19952] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-19952-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 was knocked down in HCT116 cells by four different siRNAs and the cell lysates immunoprobed as indicated. Mock and scrambled control samples are included. b-actin is used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Knockdown-Validated-NBP2-19952-img0003.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241537198.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241538418.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202415291972.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113548.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113543.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241611358.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416121996.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - A C-terminal domain in hRpn10 binds E6AP AZUL.a Positions of known functional domains within hRpn10 (top) & E6AP isoform II (bottom). Question mark “?” indicates hRpn10 uncharacterized region & the E6AP catalytic cysteine C843 is indicated. b Pull-down assay of His-tagged hRpn10full-length (full), hRpn10196–377 or hRpn10196–306 without (−) or with (+) incubation of E6AP. c1H, 15N HSQC spectra of 0.2 mM 15N-hRpn10305–377 (black) & with twofold molar excess unlabeled AZUL (green). Shifted signals are labeled. d Table summarizing Kd, kon, & koff average values with standard deviations for the hRpn10305–377: AZUL interaction measured by ITC and/or SPR. N/A, not applicable. e HCT116 lysates expressing empty vector, myc-hRpn10 full length, or myc-Rpn10 with RAZUL deleted ( delta 307–377) were subjected to myc-immunoprecipitation with anti-myc-tag nanobody-coupled agarose. Whole cell extracts (WCE) & myc-immunoprecipitates were immunoprobed with the indicated antibodies. Cyclophilin B (Cyp B) is used as a loading control in e & f. f Lysates from HCT116 cells expressing HA-E6AP & the myc-hRpn10 constructs of e were subjected to HA IP followed by immunoblotting with the indicated antibodies. An asterisk “*” indicates non-specific interaction; double asterisk “**” indicates heavy chain antibody. e–f All antibodies used for immunoprobing are indicated to the left of the images. Note that the hRpn10 & E6AP antibodies recognize both endogenous & exogenously expressed protein, causing these panels to show both tagged & endogenous protein. g Schematic representation highlighting interaction domains of hRpn10 including newly identified RAZUL. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416114714.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)")
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - KG-1 cells using CD34 Monoclonal Antibody (HPCA1/1171) (red) & isotype control (green).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Flow-Cytometry-NBP2-44569-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0002.jpg)
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Percent of EPCs (CD133 + CD34 +) reduced in the XYK group detected by flow cytometry. Data are mean +/- SD analyzed by one-way analysis of variance. **P < 0.01. CSDH, chronic subdural hematoma; EPC, endothelial progenitor cell; XYK, Xiaoyukang Jiaonang. PE conjugated CD34 antibody used (NBP2-47911PE). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32328124/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-44569_mouse-monoclonal-cd34-antibody-hpca1-1171-177202495947.jpg)
![Western Blot: Pax6 Antibody (PAX6/498) [NBP2-32893] - Analysis of PAX6 in human cerebellum lysate.](http://images.novusbio.com/fullsize/Pax6-Antibody-PAX6-498-Western-Blot-NBP2-32893-img0001.jpg "Western Blot: Pax6 Antibody (PAX6/498) [NBP2-32893] - Analysis of PAX6 in human cerebellum lysate.")
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0012.jpg "Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31555-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Rat duodenum. NAGLU antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse brain. NAGLU antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0011.jpg)
![alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) .Blue: Nuclear staining with Hoechst 33342. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31555_rabbit-polyclonal-alpha-n-acetylglucosaminidase-naglu-antibody-immunohistochemistry-paraffin-141120251843564.jpg)
![alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha-N-acetylglucosaminidase/NAGLU stained by alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp1-32244_rabbit-polyclonal-calretinin-antibody-immunohistochemistry-frozen-141120251855418.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (7711) diluted at 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (7711) diluted at 1:500.")
![<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [7711] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0001.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Glutamine Synthetase antibody [GT7711] detects Glutamine Synthetase protein in glial cells by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron & glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine Synthetase stained by Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-910202419593549.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:250000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-91020242063368.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Glutamine Synthetase antibody [GT7711] detects Glutamine Synthetase protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Glutamine Synthetase stained by Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-91020242041239.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-paraffin-26112025199277.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-frozen-261120251823624.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-western-blot-2611202518422920.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-paraffin-261120251844100.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-paraffin-261120251995415.jpg)
![Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46853-img0003.jpg "Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain")
![Immunohistochemistry: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN1 in human fetal brain](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-NBP1-46853-img0004.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16590] - Immunohistochemical analysis of paraffin-embedded Gastric carcinoma, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16590-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: A549 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0001.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysate B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0002.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - A. 50 ug rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16590] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0005.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16590] - Sandwich ELISA detection of recombinant full-length Galectin 3 protein using as detection antibody at concentration of 1 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16590-img0006.jpg)
![Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Western-Blot-NBP2-27117-img0008.jpg "Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis of decalcified, FFPE tissue sections from the paws of mice with collagen-induced arthritis using the HMGB1 antibody. The antibody was used at 5 ug/ml. Data courtesy of Dr Ulf Andersson, Karolinska Institute.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27117-img0007.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody [NBP2-27117] - Intracellular analysis using HMGB1 antibody. Human monocytes were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-27117 and APC secondary this antibody were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Flow-Cytometry-NBP2-27117-img0006.jpg)
![Simple Western: HMGB1/HMG-1 Antibody [NBP2-27117] - Lane view shows a specific band for HMGB1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Simple-Western-NBP2-27117-img0005.jpg)
![Western Blot: PGD2 Synthase/PTGDS Antibody [NB110-59909] - Detection of Prostaglandin D Synthase/Lipocalin in human brain lysates (NB820-59177) using NB110-59909.](http://images.novusbio.com/fullsize/PGD2-Synthase-PTGDS-Antibody-Western-Blot-NB110-59909-img0004.jpg "Western Blot: PGD2 Synthase/PTGDS Antibody [NB110-59909] - Detection of Prostaglandin D Synthase/Lipocalin in human brain lysates (NB820-59177) using NB110-59909.")
![Simple Western: PGD2 Synthase/PTGDS Antibody [NB110-59909] - Simple Western lane view shows a specific band for PGD2/Synthase/PTGDS in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGD2-Synthase-PTGDS-Antibody-Simple-Western-NB110-59909-img0005.jpg)
![Western Blot: Secretagogin Antibody [NBP2-20286] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Secretagogin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Western-Blot-NBP2-20286-img0009.jpg "Western Blot: Secretagogin Antibody [NBP2-20286] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Secretagogin antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Secretagogin stained by Secretagogin antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20286-img0008.jpg)
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Secretagogin protein stained by Secretagogin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0010.jpg)
![Western Blot: Secretagogin Antibody [NBP2-20286] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Western-Blot-NBP2-20286-img0005.jpg)
![Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP2-20286] - Human colon carcinoma, using SCGN antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Paraffin-NBP2-20286-img0002.jpg)
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Secretagogin protein stained by Secretagogin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0006.jpg)
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Secretagogin stained by Secretagogin antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0007.jpg)