| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82074-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86664-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92684SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54426-100ug | |||
![Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Analysis of extracts from Hela cells, treated with N oc or calf intestinal phosphates (CIP), using NFkB p105/p50 Antibody.](http://images.novusbio.com/fullsize/NFkB-p105-p50-[p-Ser337]-Antibody-Western-Blot-NB100-82074-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Analysis of extracts from Hela cells, treated with N oc or calf intestinal phosphates (CIP), using NFkB p105/p50 Antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Staining of methanol-fixed HeLa cells.](http://images.novusbio.com/fullsize/NFkB-p105-p50-[p-Ser337]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82074-img0005.jpg)
![Immunohistochemistry: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NB100-82074.](http://images.novusbio.com/fullsize/nb100-82074_rabbit-polyclonal-nfkb-p105-p50-p-ser337-antibody-10320252332101.jpg)
![Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Western blot analysis of extract from HeLa cells, using NB100-82074 (Lane 1 and 2).](http://images.novusbio.com/fullsize/nb100-82074_rabbit-polyclonal-nfkb-p105-p50-p-ser337-antibody-103202523472637.jpg)
![Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Western-Blot-NBP2-75632-img0009.jpg "Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.")
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0003.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0008.jpg)
![Flow Cytometry: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Flow-Cytometry-NBP2-75632-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0002.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0004.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0005.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0006.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0007.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Anaysis of Lgr5/GPR49 with Brain, hippocampus](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0015.jpg "Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Anaysis of Lgr5/GPR49 with Brain, hippocampus")
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of anti-GPR49 antibody with human, small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0011.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of anti-GPR49 antibody with human colon.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0012.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of Lgr5/GPR49 with brain, glioblastoma](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0013.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of Lgr5/GPR49 with Brain, Caudate](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0014.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NLS1236] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human Brain, Cortex tissue](http://images.novusbio.com/fullsize/nls1236_rabbit-lgr5-gpr49-pab-bsa-free-10920241103115.jpg)
![Western Blot: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control: Line 1: MCF-7 Line 2: A549](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Western-Blot-NBP2-66982-img0004.jpg "Western Blot: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control: Line 1: MCF-7 Line 2: A549")
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Staining NMDAR1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunocytochemistry-Immunofluorescence-NBP2-66982-img0005.jpg)
![Immunohistochemistry-Paraffin: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of paraffin-embedded rat brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunohistochemistry-Paraffin-NBP2-66982-img0003.jpg)
![Flow Cytometry: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of SH-SY5Y cells with NMDAR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Flow-Cytometry-NBP2-66982-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Staining NMDAR1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunocytochemistry-Immunofluorescence-NBP2-66982-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Staining NMDAR1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunocytochemistry-Immunofluorescence-NBP2-66982-img0006.jpg)
![Immunohistochemistry-Paraffin: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of paraffin-embedded mouse brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunohistochemistry-Paraffin-NBP2-66982-img0002.jpg)
![Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on mouse liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Western-Blot-NBP2-67572-img0007.jpg "Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on mouse liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of paraffin-embedded mouse brain tissue using anti-S100 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunohistochemistry-Paraffin-NBP2-67572-img0003.jpg)
![Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Western-Blot-NBP2-67572-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0002.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Western-Blot-NBP2-54787-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde made from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54787-img0006.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to when 100 uM of active alpha Synuclein monomer (NBP2-54788) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787), or 100 uM of control alpha Synuclein monomer (NBP2-54786) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787). Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-In-vitro-assay-NBP2-54787-img0007.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) (Catalog No. NBP2-54787) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Product-Image-NBP2-54787-img0009.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - TEM of Type 2 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54787)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Electron-Microscopy-NBP2-54787-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86664-img0012.jpg "Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.")
![Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF-1 alpha and BICD1. Cells were pretreated with MG132 (1 uM) for 30 min prior to hypoxia treatment for 24 h. Co-immunoprecipitation of HIF-1 alpha with IgG and BICD1 were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-018-0241-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0004.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Role of BICD1 in UCB-MSCs survival under hypoxia. a, b The UCB-MSCs were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment. a Cells were exposed to various durations of hypoxia (0–72 h). Cell viability of UCB-MSCs was measured by WST-1 cell viability assay. n = 8. b Cells were incubated in normoxia or hypoxia conditions for 72 h. Representative images of experimental groups at 0, 24, 48, & 72 h of normoxia or hypoxia incubation are presented. Red-marked cells in representative images indicate PI-positive cells. n = 4. Scale bars are 50 μm (Magnification, × 200). cBICD1 or NT siRNA-transfected cells were incubated in hypoxia for 24 h. Cleaved caspase-9, cleaved caspase-3, & beta -Actin protein expressions were detected by western blot analysis. n = 4. d Cells were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 48 h. The percentages of apoptotic cells were analyzed by Annexin V/PI analysis, measured by flowcytometer. Annexin V-positive cells were considered as apoptotic cells. n = 4. Quantitative data are presented as a mean ± S.E.M. All blot images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection. e The UCB-MSCs were transfected with pcDNA3.1/BICD1-cEGFP vector, pcDNA3.1/cEGFP vector, HIF1A siRNA or NT siRNA for 24 h prior to hypoxia treatment for 72 h. Cell viability data are presented as a mean ± S.E.M. n = 5. *p < 0.05 vs. normoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, #p < 0.05 vs. hypoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, @p < 0.05 vs. hypoxia control with pcDNA3.1/BICD1-cEGFP vector & NT siRNA cotransfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171413.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171451.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616184.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Involvement of Akt/GSK3 beta pathway in BICD1-mediated HIF1 alpha nuclear translocation. a–e The UCB-MSCs were transfected with GSK3 beta or NT siRNA for 24 h prior to hypoxia treatment for 24 h. a Co-immunoprecipitation of HIF1 alpha & Dynein IC with IgG & BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. b Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) was analyzed by PLA assay. Scale bars are 8 μm (Magnification, × 1,000). n = 5. c HIF1 alpha , Lamin A/C, & alpha -Tubulin in cytosolic & nuclear fractionized samples were detected by western blot. n = 4. d Cells were immunostained with HIF1 alpha -specific antibody. Scale bars are 8 μm (Magnification, × 1,000). n = 4. e HIF1 activities in NT or GSK3 beta siRNA-transfected cells were analyzed by dual luciferase reporter assay. n = 6. Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616183.jpg)
![Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.](http://images.novusbio.com/fullsize/Abeta-40-Antibody-Western-Blot-NBP1-44047-img0002.jpg "Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.")
![Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Western-Blot-NBP1-92684-img0005.jpg "Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0003.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with LIN-28A antibody diluted at 1:500.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0008.jpg "Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with LIN-28A antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (717) [NBP2-43672] - Analysis of human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: LIN28 protein stained by LIN28 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Immunocytochemistry-Immunofluorescence-NBP2-43672-img0002.jpg)
![Immunohistochemistry: LIN-28A Antibody (717) [NBP2-43672] - Analysis of U87 xenograft. Lin28A antibody dilution: 1:100.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Immunohistochemistry-NBP2-43672-img0007.jpg)
![Flow Cytometry: LIN-28A Antibody (717) [NBP2-43672] - Analysis of Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Lin28A antibody [717] dilution: 1:50](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Flow-Cytometry-NBP2-43672-img0001.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Analysis of 30 ug whole cell lysate. A: Non-transfected HeLa lysates B: LIN28 transfected HeLa lysates 12% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0004.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Analysis of 30 ug whole cell lysate. A: NT2D1 12% SDS PAGE diluted at 1:1000. .](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0005.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Analysis of A. 30 ug human ESC whole cell lysate/extract 12 % SDS-PAGE Lin28A antibody [717] dilution: 1:1000](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0006.jpg)
![Western Blot: S100B Antibody (S100B/1706R) [NBP2-53188] - Western blot analysis of human brain tissue lysate using S100B antibody (S100B/1706R).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Western-Blot-NBP2-53188-img0003.jpg "Western Blot: S100B Antibody (S100B/1706R) [NBP2-53188] - Western blot analysis of human brain tissue lysate using S100B antibody (S100B/1706R).")
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1706R) [NBP2-53188] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Rabbit Monoclonal Antibody (S100B/1706R).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Immunohistochemistry-Paraffin-NBP2-53188-img0001.jpg)
![Protein Array: S100B Antibody (S100B/1706R) [NBP2-53188] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100B Antibody (S100B/1706R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Protein-Array-NBP2-53188-img0002.jpg)