| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47911-0.1mg | |||
| IHC-Prediluted | NBP2-48363 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82001-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21180-25ul | |||
| Azide and BSA Free | NBP3-44262 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82527-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41264-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41262-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74910 | |||
![Western Blot: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19460-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: N Cadherin Antibody [NBP2-19460] - Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19460-img0002.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19460] - Paraffin-embedded hepatoma. N-Cadherin antibody [N2C1], Internal dilution: 1:500.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19460-img0003.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19460] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-91020242014643.jpg)
![N-Cadherin antibody [N2C1], Internal detects N-Cadherin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-immunohistochemistry-frozen-261120251842318.jpg)
![Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251828100.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251846100.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Western-Blot-NBP2-19952-img0004.jpg "Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP2-19952] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19952-img0002.jpg)
![Western Blot: Proteasome 19S S5A Antibody [NBP2-19952] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-19952-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 was knocked down in HCT116 cells by four different siRNAs and the cell lysates immunoprobed as indicated. Mock and scrambled control samples are included. b-actin is used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Knockdown-Validated-NBP2-19952-img0003.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241537198.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241538418.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202415291972.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113548.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113543.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241611358.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416121996.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - A C-terminal domain in hRpn10 binds E6AP AZUL.a Positions of known functional domains within hRpn10 (top) & E6AP isoform II (bottom). Question mark “?” indicates hRpn10 uncharacterized region & the E6AP catalytic cysteine C843 is indicated. b Pull-down assay of His-tagged hRpn10full-length (full), hRpn10196–377 or hRpn10196–306 without (−) or with (+) incubation of E6AP. c1H, 15N HSQC spectra of 0.2 mM 15N-hRpn10305–377 (black) & with twofold molar excess unlabeled AZUL (green). Shifted signals are labeled. d Table summarizing Kd, kon, & koff average values with standard deviations for the hRpn10305–377: AZUL interaction measured by ITC and/or SPR. N/A, not applicable. e HCT116 lysates expressing empty vector, myc-hRpn10 full length, or myc-Rpn10 with RAZUL deleted ( delta 307–377) were subjected to myc-immunoprecipitation with anti-myc-tag nanobody-coupled agarose. Whole cell extracts (WCE) & myc-immunoprecipitates were immunoprobed with the indicated antibodies. Cyclophilin B (Cyp B) is used as a loading control in e & f. f Lysates from HCT116 cells expressing HA-E6AP & the myc-hRpn10 constructs of e were subjected to HA IP followed by immunoblotting with the indicated antibodies. An asterisk “*” indicates non-specific interaction; double asterisk “**” indicates heavy chain antibody. e–f All antibodies used for immunoprobing are indicated to the left of the images. Note that the hRpn10 & E6AP antibodies recognize both endogenous & exogenously expressed protein, causing these panels to show both tagged & endogenous protein. g Schematic representation highlighting interaction domains of hRpn10 including newly identified RAZUL. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416114714.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)")
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - KG-1 cells using CD34 Monoclonal Antibody (HPCA1/1171) (red) & isotype control (green).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Flow-Cytometry-NBP2-44569-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0002.jpg)
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Percent of EPCs (CD133 + CD34 +) reduced in the XYK group detected by flow cytometry. Data are mean +/- SD analyzed by one-way analysis of variance. **P < 0.01. CSDH, chronic subdural hematoma; EPC, endothelial progenitor cell; XYK, Xiaoyukang Jiaonang. PE conjugated CD34 antibody used (NBP2-47911PE). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32328124/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-44569_mouse-monoclonal-cd34-antibody-hpca1-1171-177202495947.jpg)
![Western Blot: IRS1 Antibody [NB100-82001] - Analysis of extracts from 293 cells using NB100-82001](http://images.novusbio.com/fullsize/IRS1-Antibody-Western-Blot-NB100-82001-img0002.jpg "Western Blot: IRS1 Antibody [NB100-82001] - Analysis of extracts from 293 cells using NB100-82001")
![Immunocytochemistry/ Immunofluorescence: IRS1 Antibody - BSA Free [NB100-82001] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-82001.](http://images.novusbio.com/fullsize/nb100-82001_rabbit-polyclonal-irs1-antibody-103202523464533.jpg)
![Immunohistochemistry-Paraffin: IRS1 Antibody [NB100-82001] - Analysis of breast carcinoma tissue, using NB100-82001(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-82001_rabbit-polyclonal-irs1-antibody-103202523321013.jpg)
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer).](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NB110-74960-img0005.jpg "Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer).")
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer)](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NB110-74960-img0003.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518341523.jpg "Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded rat esophagus.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-1411202518382820.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518435619.jpg)
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-141120251841520.jpg)
![AKT antibody [HL2915] detects AKT protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunocytochemistry-immunofluorescence-1411202518424111.jpg)
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-1411202518312110.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518394118.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518334523.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Analysis in human cerebral cortex and cervix, uterine tissues using NBP2-33774 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-33774_rabbit-polyclonal-gfap-antibody-immunohistochemistry-paraffin-166202374820.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Analysis in human cerebral cortex and cervix, uterine tissues using NBP2-33774 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GFAP Antibody [NBP2-33774] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NBP2-33774-img0043.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NBP2-33774] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33774-img0017.jpg)
![Western Blot: GFAP Antibody [NBP2-33774] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NBP2-33774-img0016.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] - Staining of human hippocampus shows positivity in astrocytes.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-33774-img0039.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] - Staining of human pancreas shows no positivity as expected.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-33774-img0040.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Staining of human cervix, uterine shows no cytoplasmic positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-33774_rabbit-polyclonal-gfap-antibody-immunohistochemistry-paraffin-166202375644..jpg)
![Immunohistochemistry-Paraffin: NELL2 Antibody [NBP1-82527] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/NELL2-Antibody-Immunohistochemistry-Paraffin-NBP1-82527-img0008.jpg "Immunohistochemistry-Paraffin: NELL2 Antibody [NBP1-82527] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.")
![Immunohistochemistry: NELL2 Antibody [NBP1-82527] - NELL2 expression in the 3% PFA perfused mouse brain Hippocampus and Cortex using primary antibody with 1:100 dilution. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NELL2-Antibody-Immunohistochemistry-NBP1-82527-img0004.jpg)
![Immunohistochemistry-Paraffin: NELL2 Antibody [NBP1-82527] - Staining of human placenta shows very weak cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/NELL2-Antibody-Immunohistochemistry-Paraffin-NBP1-82527-img0005.jpg)
![Immunohistochemistry-Paraffin: NELL2 Antibody [NBP1-82527] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/NELL2-Antibody-Immunohistochemistry-Paraffin-NBP1-82527-img0007.jpg)
![Immunohistochemistry-Paraffin: NELL2 Antibody [NBP1-82527] - Staining of human Fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NELL2-Antibody-Immunohistochemistry-Paraffin-NBP1-82527-img0006.jpg)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - KO Validation in HeLa Cells.Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-271120241932699.gif "<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - KO Validation in HeLa Cells.Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Mouse Tissues.Loading: 15 ul of lysate Antibodies: HMGB1/HMG-1 , 1 ug/mL , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420405564.gif)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Rat Tissues.Loading: 15 ug of lysate Antibodies: HMGB1/HMG-1, 1 u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-271120242074672.gif)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysate Antibodies: HMGB1/HMG-1, 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420392765.gif)
![Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in HL60 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed HL60 cells labeling HMGB1/HMG-1 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202419432287.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Human Stomach Tissue.Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-HMGB1/HMG-1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420312979.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Mouse Spleen Tissue.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HMGB1/HMG-1 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420392762.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-HMGB1/HMG-1 antibody at 1 ul/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420333866.jpg)
![Western Blot: TRPV4 Antibody [NBP2-41262] - Analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/TRPV4-Antibody-Western-Blot-NBP2-41262-img0005.jpg "Western Blot: TRPV4 Antibody [NBP2-41262] - Analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 ug/mL.")
![Immunohistochemistry: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunohistochemistry of TRPV4 in human lung tissue with TRPV4 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-2711202419553050.jpg)
![Immunohistochemistry: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunohistochemistry of TRPV4 in human kidney tissue with TRPV4 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-2711202419553036.gif)
![Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-271120242004123.jpg)
![Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunofluorescence of TRPV4 in human kidney tissue with TRPV4 antibody at 5 ug/ml.Red: TRPV4 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-271120242004135.gif)
![Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Cross-reactivity test with ZEB2: Analysis of HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right).](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Western-Blot-NBP2-46398-img0007.jpg "Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Cross-reactivity test with ZEB2: Analysis of HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right).")
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0006.jpg)
![Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Western-Blot-NBP2-46398-img0008.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0001.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0002.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0003.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0004.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0005.jpg)