| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90080-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88697-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71771SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25093-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74245 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76910-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37261-0.025ml | |||
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0003.jpg "Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Mouse fore brain. SNAP25 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0006.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Rat hind brain. SNAP25 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0010.jpg)
![Immunohistochemistry-Whole-Mount: SNAP25 Antibody [NBP1-33714] - Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: SNAP25 stained by SNAP25 antibody diluted at 1:50.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70 C](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunohistochemistry-whole-mount-127202384126.jpg)
![Western Blot: SNAP25 Antibody [NBP1-33714] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-western-blot-12720238431..jpg)
![Western Blot: SNAP25 Antibody [NBP1-33714] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-western-blot-127202384456..jpg)
![SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: SNAP25 protein stained by SNAP25 antibody (NBP1-33714) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunocytochemistry-immunofluorescence-261120251832456.jpg)
![SNAP25 antibody detects SNAP25 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: SNAP25 protein stained by SNAP25 antibody (NBP1-33714) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunohistochemistry-frozen-261120251829497.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP1-90080] - Staining of human cerebral cortex shows moderate positivity.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-Immunohistochemistry-Paraffin-NBP1-90080-img0013.jpg)
![Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP1-90080] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-Immunohistochemistry-Paraffin-NBP1-90080-img0014.jpg)
![Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP1-90080] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-Immunohistochemistry-Paraffin-NBP1-90080-img0012.jpg)
![Immunohistochemistry-Paraffin: Neuroligin 3/NLGN3 Antibody [NBP1-90080] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-Immunohistochemistry-Paraffin-NBP1-90080-img0015.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - S1P inhibition induces autophagy and affects multiple UPR-related TFs. In vitro day 3 B-cells were treated with 10 uM PF-429242 or vehicle control (dH2O) for 72 h and were assessed for cell number using CountBright beads. Shown are the average cell numbers from 7 donors +/- standard deviations. Protein lysates generated from day 3 cells treated for 72 h with 10 uM PF-429242 were evaluated for CREB3L2 and ATF6 by Western blotting. Shown are representative results from 2 donors, a total of 7 donors were evaluated. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-32705-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP1-88697-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: CREB3L2 Antibody [NBP1-88697] - S1P inhibition induces autophagy and affects multiple UPR-related TFs. In vitro day 3 B-cells were treated with 10 uM PF-429242 or vehicle control (dH2O) for 72 h and were assessed for cell number using CountBright beads. Shown are the average cell numbers from 7 donors +/- standard deviations. Protein lysates generated from day 3 cells treated for 72 h with 10 uM PF-429242 were evaluated for CREB3L2 and ATF6 by Western blotting. Shown are representative results from 2 donors, a total of 7 donors were evaluated. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-32705-7), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0011.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - Analysis in control (vector only transfected HEK293T lysate) and CREB3L2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP1-88697-img0006.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - Expression of CREB3L2 in differentiating primary human B-cells. Protein expression of CREB3L2 during in vitro differentiation of primary human B-cells. The day of culture is indicated at the top. Intact and dashed arrows show migration of full-length protein and cleaved C-terminus, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-32705-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP1-88697-img0007.jpg)
![Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human kidney shows strong cytoplasmci positivity in cells in tubules.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0009.jpg)
![Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0010.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - Kinetic & dose analysis of S1P-specific inhibitor PF-429242 during the early phase of ASC generation. (A) In vitro activated human B-cells were treated with 10 μM PF-429242 or vehicle control (dH2O) at day 3 & assessed for CREB3L2 processing at 12 h intervals. Representative results are shown from a total of 7 donors. (B) Samples from part (A) were assessed for cell number at the indicated time points. Displayed are the average cell number ± standard deviation derived from 7 donors. (C) In vitro activated human B-cells from 4 donors were treated with indicated amount PF-429242 or vehicle control (dH2O) at day 3 & assessed for cell number at 48 h. Results are displayed as the percentage viable cells relative to control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30254311), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88697_rabbit-polyclonal-creb3l2-antibody-310202415382439.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - S1P-specific inhibitor PF-429242 blocks CREB3L2 processing. (A) Diagram of CREB3L2 structure with proteolytic cleavage sites. Act domain, Activation Domain. bZIP, Basic Leucine Zipper Domain. TM, Transmembrane Domain. (B) In vitro generated human ASCs were treated with 10 μM PF-429242 or vehicle control (dH2O) on the day that appears first (d6, d8, d10) & protein lysates generated on the day that appears subsequently (d8, d10, d13) were analyzed by Western blotting. Intact & dashed arrows show migration of CREB3L2 full-length protein & cleaved C-terminus, respectively. (C) In vitro differentiated human B-cells were treated with 10 μM PF-429242 or vehicle control (dH2O) at day 6 & analyzed subsequently for phenotype on the indicated days. Live cells were evaluated for expression of B-cell markers CD19 & CD20 & ASC markers CD38 & CD138. Percentages of cells are shown within the quadrant gates. The experiment was performed with 4 donors, a representative donor is shown. (D) Samples treated as in part (B) were assessed for cell number using CountBright beads. (E) In vitro activated human B-cells from 4 donors were treated with indicated amount PF-429242 or vehicle control (dH2O) at day 6 & assessed for cell number at 48 h. Results are displayed as the percentage viable cells relative to control. Supernatants from samples in part (B) were analyzed by ELISA for production of (F) IgM & (G) IgG. Significance was determined by t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30254311), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88697_rabbit-polyclonal-creb3l2-antibody-310202416121935.jpg)
![Immunocytochemistry/Immunofluorescence: GABARAP Antibody [NBP1-71771] - analysis of GABARAP in Raw 264.7 cells using anti-GABARAP antibody (red). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/GABARAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71771-img0008.jpg "Immunocytochemistry/Immunofluorescence: GABARAP Antibody [NBP1-71771] - analysis of GABARAP in Raw 264.7 cells using anti-GABARAP antibody (red). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: GABARAP Antibody [NBP1-71771] - Analysis of GABARAP in mouse liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/GABARAP-Antibody-Immunohistochemistry-NBP1-71771-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GABARAP Antibody [NBP1-71771] - GABARAP antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/GABARAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71771-img0005.jpg)
![Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - KO Validation in 293T Cells Loading: 10 ug of lysate Antibodies: beta Amyloid, 0.5 ug/mL and beta-actin, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-2711202419333416.gif "<b>Genetic Strategies Validation. </b>Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - KO Validation in 293T Cells Loading: 10 ug of lysate Antibodies: beta Amyloid, 0.5 ug/mL and beta-actin, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunohistochemistry of beta Amyloid in human brain tissue with beta Amyloid antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120241918631.gif)
![Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunohistochemistry of beta Amyloid in human brain (Alzheimer's disease) tissue with beta Amyloid antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120242016992.jpg)
![Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 ug/mL.Green: beta Amyloid Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120241939277.gif)
![Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - Western blot analysis of beta Amyloid in (A) human, (B) mouse, and (C) rat brain tissue lysates with beta Amyloid antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120242022942.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0008.jpg "Western Blot: APP Antibody [NBP2-15575] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575] - Rat brain. APP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP2-15575-img0003.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0004.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0006.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0007.jpg)
![ELISA: APP Antibody [NBP2-15575] - Detection of non-transfected and transfected 293T whole cell extracts using NBP2-15575 as capture antibody at concentration of 5 ug/mL and a mouse monoclonal anti-APP antibody as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/APP-Antibody-ELISA-NBP2-15575-img0013.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Analysis in human cerebral cortex and testis tissues using NBP1-88769 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Analysis in human cerebral cortex and testis tissues using NBP1-88769 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in human cell lines U2OS and MCF-7 using anti-SNAP25 antibody. Corresponding SNAP25 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NBP1-88769-img0017.jpg)
![Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NBP1-88769-img0025.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0020.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0021.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0022.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0023.jpg)
![Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Western-Blot-NBP2-00795-img0002.jpg "Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.")
![Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4G5) [NBP2-00795] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunocytochemistry-Immunofluorescence-NBP2-00795-img0009.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human liver tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0008.jpg)
![Flow Cytometry: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAP25 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Flow-Cytometry-NBP2-00795-img0001.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0003.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0004.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0005.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0006.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human colon tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0007.jpg)
![Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-Western-Blot-NBP1-76910-img0012.jpg "Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: APP Antibody - BSA Free [NBP1-76910] - Immunofluorescence of APP in Rat Brain cells with APP antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419451253.jpg)
![Immunocytochemistry/ Immunofluorescence: APP Antibody - BSA Free [NBP1-76910] - Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 ug/mL. Green: APP antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419541571.gif)
![Immunohistochemistry: APP Antibody - BSA Free [NBP1-76910] - Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419251561.gif)
![Immunohistochemistry: APP Antibody - BSA Free [NBP1-76910] - Immunohistochemical staining of rat brain using APP antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-271120241932669.jpg)
![Western Blot: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of 75 ug of various whole cell lysates and 10 ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Western-Blot-NBP2-22481-img0001.jpg "Western Blot: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of 75 ug of various whole cell lysates and 10 ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 in NCCIT and HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a LIN28 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0005.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the cytoplasm of human seminoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunohistochemistry-Paraffin-NBP2-22481-img0015.jpg)
![Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on H9 embryonic stem cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Flow-Cytometry-NBP2-22481-img0013.jpg)
![Chromatin Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis performed using cross-linked chromatin from rat hepatoma cells treated with insulin. IP performed using a multiplex microplate Matrix ChIP assay of LIN28 monoclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2-3 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the rat Egr-1 locus is shown; oxes represent exons (black boxes = translated, white boxes = untranslated), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Chromatin-Immunoprecipitation-ChIP-NBP2-22481-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0004.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the nucleus and cytoplasm of mouse testis tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunohistochemistry-Paraffin-NBP2-22481-img0014.jpg)
![Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on HEL 11.4 induced IPS cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Flow-Cytometry-NBP2-22481-img0012.jpg)
![Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 was performed. Antigen-antibody complexes were formed by incubating 700ug of lysate with 5 ug of an LIN28 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Agarose was loaded as a positive control for detection. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a LIN28 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunoprecipitation-NBP2-22481-img0002.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0004.jpg "Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Immunocytochemistry-Immunofluorescence-NBP2-37261-img0005.jpg)
![Flow Cytometry: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Flow-Cytometry-NBP2-37261-img0006.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mAb against human BACE1 (AA: 112-324) recombinant protein. (Expected MW is 49.9 kDa)](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0002.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mAb against HEK293 (1) and BACE1 (AA: 112-324)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0003.jpg)
![ELISA: BACE-1 Antibody (3C1C3) [NBP2-37261] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-ELISA-NBP2-37261-img0001.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
