| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85438-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78133SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90205-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13426-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24138 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03768-20ul | |||
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0012.jpg "Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0002.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney, liver, placenta and testis using Anti-LIN28B antibody NBP1-85438 (A) shows similar protein distribution across tissues to independent antibody NBP2-32352 (B).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0005.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human liver.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0006.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human testis.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0007.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Analysis in human placenta and prostate tissues. Corresponding LIN-28B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney, liver, placenta and prostate using Anti-LIN-28B antibody NBP1-85438 (A) shows similar protein distribution across tissues to independent antibody NBP2-32352 (B).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0010.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0011.jpg)
![Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Western-Blot-NBP2-78133-img0002.jpg "Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NBP2-78133] - IHC analysis of a FFPE tissue section of the mouse testis using 1:200 dilution of SCP3 antibody (NBP2-78133). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated strong staining associated with chromatin in meiotic cells.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunohistochemistry-Paraffin-NBP2-78133-img0001.jpg)
![Western Blot: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with HL60 cell line lysates (35ug/lane).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Western-Blot-NBP1-50529-img0001.jpg "Western Blot: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with HL60 cell line lysates (35ug/lane).")
![Immunocytochemistry/Immunofluorescence: LIN-28B Antibody [NBP1-50529] - Fluorescent confocal image of SY5Y cells stained with LIN28B antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LIN28B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LIN28B immunosignal to the cytoplasm.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-50529-img0008.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-50529] - Human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-50529-img0006.jpg)
![Flow Cytometry: LIN-28B Antibody [NBP1-50529] - Flow cytometric of HL-60 cells using LIN28B Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Flow-Cytometry-NBP1-50529-img0010.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with human spleen at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-50529-img0003.jpg)
![Western Blot: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of CREB3L4 over-expressed 293 cell line, cotransfected with CREB3L4 Validated Chimera RNAi ( Cat # H00148327-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CREB3L4 monoclonal antibody (M01), clone 3E3 (Cat # H00148327-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-3E3-Western-Blot-H00148327-M01-img0008.jpg "Western Blot: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of CREB3L4 over-expressed 293 cell line, cotransfected with CREB3L4 Validated Chimera RNAi ( Cat # H00148327-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CREB3L4 monoclonal antibody (M01), clone 3E3 (Cat # H00148327-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Immunocytochemistry/Immunofluorescence: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of monoclonal antibody to CREB3L4 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-3E3-Immunocytochemistry-Immunofluorescence-H00148327-M01-img0007.jpg)
![Western Blot: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of CREB3L4 expression in transfected 293T cell line by CREB3L4 monoclonal antibody (M01), clone 3E3.Lane 1: CREB3L4 transfected lysate(43.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-3E3-Western-Blot-H00148327-M01-img0009.jpg)
![Proximity Ligation Assay: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of protein-protein interactions between CREB3 and CREB3L4. HeLa cells were stained with anti-CREB3 rabbit purified polyclonal 1:1200 and anti-CREB3L4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AIBZIP-Antibody-3E3-Proximity-Ligation-Assay-H00148327-M01-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AIBZIP Antibody [NBP1-90205] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear membrane & mitochondria.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90205-img0004.jpg "Immunocytochemistry/Immunofluorescence: AIBZIP Antibody [NBP1-90205] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear membrane & mitochondria.")
![Immunohistochemistry-Paraffin: AIBZIP Antibody [NBP1-90205] - Staining of human prostate shows high expression.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-Immunohistochemistry-Paraffin-NBP1-90205-img0007.jpg)
![Immunohistochemistry-Paraffin: AIBZIP Antibody [NBP1-90205] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-Immunohistochemistry-Paraffin-NBP1-90205-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: tescalcin Antibody [NBP2-13426] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/tescalcin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13426-img0003.jpg "Immunocytochemistry/Immunofluorescence: tescalcin Antibody [NBP2-13426] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: tescalcin Antibody [NBP2-13426] - Staining of human colon shows low expression as expected.](http://images.novusbio.com/fullsize/tescalcin-Antibody-Immunohistochemistry-Paraffin-NBP2-13426-img0007.jpg)
![Immunohistochemistry-Paraffin: tescalcin Antibody [NBP2-13426] - Staining of human salivary gland shows high expression.](http://images.novusbio.com/fullsize/tescalcin-Antibody-Immunohistochemistry-Paraffin-NBP2-13426-img0008.jpg)
![Western Blot: AIBZIP Antibody [NB100-1015] - Staining (0.5ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-Western-Blot-NB100-1015-img0006.jpg "Western Blot: AIBZIP Antibody [NB100-1015] - Staining (0.5ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: AIBZIP Antibody [NB100-1015] - IHC analysis of AIBZIP in paraffin embedded Human Prostate using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-Immunohistochemistry-Paraffin-NB100-1015-img0005.jpg)
![Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815] - Western blot analysis of extracts of various cell lines, using LIN-28B Rabbit mAb (NBP3-16815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-7D7F7-Western-Blot-NBP3-16815-img0002.jpg "Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815] - Western blot analysis of extracts of various cell lines, using LIN-28B Rabbit mAb (NBP3-16815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry of paraffin-embedded human placenta using LIN-28B Rabbit mAb (NBP3-16815) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-7D7F7-Immunohistochemistry-Paraffin-NBP3-16815-img0001.jpg)
![Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using LIN-28B Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16815_rabbit-lin-28b-mab-7d7f7-5220251242577.jpg)
![Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry analysis of paraffin-embedded Human seminoma tissue using LIN-28B Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16815_rabbit-lin-28b-mab-7d7f7-8220252010493.jpg)
![Western Blot: LIN-28B Antibody [NBP3-13029] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LIN-28B antibody (NBP3-13029) diluted at 1:1000.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Western-Blot-NBP3-13029-img0001.jpg "Western Blot: LIN-28B Antibody [NBP3-13029] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LIN-28B antibody (NBP3-13029) diluted at 1:1000.")
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029] - LIN-28B antibody detects LIN-28B protein at cytoplasm and nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. LIN-28B antibody (NBP3-13029) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP3-13029-img0003.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029] - LIN-28B antibody detects LIN-28B protein at cytoplasm and nucleus in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon. LIN-28B antibody (NBP3-13029) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP3-13029-img0002.jpg)
![Western Blot: espin Antibody [NBP3-03768] - Analysis of extracts of various cell lines, using espin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/espin-Antibody-Western-Blot-NBP3-03768-img0001.jpg "Western Blot: espin Antibody [NBP3-03768] - Analysis of extracts of various cell lines, using espin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunohistochemistry-Paraffin: espin Antibody [NBP3-03768] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using espin Rabbit pAb (NBP3-03768) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/espin-Antibody-Immunohistochemistry-Paraffin-NBP3-03768-img0002.jpg)
![Western Blot: OCT4 Antibody [NBP2-59164] - Nuclear extracts (15 ug) from human embryonic stem cells were analysed by Western blot using the antibody against OCT4 diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-59164-img0001.jpg "Western Blot: OCT4 Antibody [NBP2-59164] - Nuclear extracts (15 ug) from human embryonic stem cells were analysed by Western blot using the antibody against OCT4 diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: OCT4 Antibody [NBP2-59164] - ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the antibody against OCT4 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/OCT4-Antibody-Chromatin-Immunoprecipitation-NBP2-59164-img0003.jpg)
![ELISA: OCT4 Antibody [NBP2-59164] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against OCT4. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:100,000.](http://images.novusbio.com/fullsize/OCT4-Antibody-ELISA-NBP2-59164-img0002.jpg)