| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61121-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB13382-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47338-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41288-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03702-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56605SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21169-25ul | |||
| Azide and BSA Free | NBP3-44275 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38508-25ul | |||
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-61596-img0003.jpg "Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.")
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-61596-img0002.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Fibrils were sonicated and image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0005.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0004.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TH fluorescence in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-61596_recombinant-mouse-alpha-synuclein-active-pre-formed-fibrils-type-1-protein-immunohistochemistry-205202512497..jpg)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP2-61121-img0004.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Phospho serine 129 antibody (NBP2-61121) was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B). Read the protocol at pabmabs.com/?p=7901. Image courtesy of Trine Rasmussen, Simon Molgaard Jensen at Aarhus University.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunohistochemistry-NBP2-61121-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 g/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:500 for 24 hours at 4C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Ser129] Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0007.jpg)
![Western Blot: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Western-Blot-NBP2-70854-img0001.jpg "Western Blot: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti")
![Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Human Kidney tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70854-img0004.jpg)
![Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Glutathione Synthetase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70854-img0002.jpg)
![Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70854-img0003.jpg)
![Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70854-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (970106) [MAB13382] - Analysis using immersion fixed SK-Mel-28 human cytoplasm using SNCA antibody.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-970106-Immunocytochemistry-Immunofluorescence-MAB13382-img0001.jpg "Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (970106) [MAB13382] - Analysis using immersion fixed SK-Mel-28 human cytoplasm using SNCA antibody.")
![Western Blot: pan-Synuclein Antibody [NBP2-47338] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-SNCA antibody. Corresponding SNCA RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Western-Blot-NBP2-47338-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: pan-Synuclein Antibody [NBP2-47338] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-SNCA antibody. Corresponding SNCA RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP2-47338] - Staining in human cerebral cortex and liver tissues using anti-SNCA antibody. Corresponding SNCA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-47338-img0007.jpg)
![Western Blot: pan-Synuclein Antibody [NBP2-47338] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Western-Blot-NBP2-47338-img0002.jpg)
![Immunohistochemistry: pan-Synuclein Antibody [NBP2-47338] - Staining of human cerebral cortex shows strongly immunoreactivity neuropil and weak cytoplasmic positivity in neuronal cells, glial cells.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-NBP2-47338-img0001.jpg)
![Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP2-47338] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-47338-img0005.jpg)
![Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP2-47338] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-47338-img0006.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0004.jpg "Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.")
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (C) 5 ug alpha synuclein PFFs Inset: PBS (negative control). Primary antibody: Anti-alpha-Synuclein (NBP3-18266) at 1:10 000. Secondary antibody: anti-rabbit HRP. Mice injected with PFF displayed alpha synuclein staining in the striatum and cortex and contralateral to the injection site.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0002.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Mouse Brain showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: Mouse brain. Load: 15 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Multiple bands due to phosphorylation.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0005.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). C57/BL6 mice were injected with 5 ug sonicated mouse recombinant alpha synuclein PFFs at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) contralateral cortex. (B) ipsilateral cortex. (C) contralateral striatum. (D) ipsilateral striatum. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:10000. Secondary Antibody: anti-rabbit HRP. Courtesy of: Porsolt.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0001.jpg)
![Dot Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Dot Blot analysis using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Tissue: alpha synuclein peptide. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Phospho peptide sequence: AYEMP-pS-EEGYQ. Non-phospho peptide sequence: AYEMPSEEGYQ. This sequence is the same for human, mouse, and rat.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Dot-Blot-NBP3-18266-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody [NBP2-41288] - Analysis of 1 ng of recombinant human SNCA with SNCA antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-41288-img0004.jpg "Western Blot: alpha-Synuclein Antibody [NBP2-41288] - Analysis of 1 ng of recombinant human SNCA with SNCA antibody at 1 ug/ml.")
![Western Blot: alpha-Synuclein Antibody [NBP2-41288] - Western blot analysis of SNCA in mouse cerebellum tissue lysate with SNCA antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-41288-img0001.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody - BSA Free [NBP2-41288] - Immunohistochemistry of alpha-Synuclein in rat brain tissue with alpha-Synuclein antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41288_rabbit-polyclonal-alpha-synuclein-antibody-2711202419241375.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Synuclein Antibody - BSA Free [NBP2-41288] - Immunofluorescence of alpha-Synuclein in rat brain tissue with alpha-Synuclein antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41288_rabbit-polyclonal-alpha-synuclein-antibody-27112024201327.jpg)
![Western Blot: Glutathione Synthetase Antibody [NBP3-03702] - WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Western-Blot-NBP3-03702-img0003.jpg "Western Blot: Glutathione Synthetase Antibody [NBP3-03702] - WB image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Western Blot: Glutathione Synthetase Antibody [NBP3-03702] - Analysis of extracts from normal (control) and GSS knockout (KO) 293T cells, using Glutathione Synthetase antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Knockout-Validated-NBP3-03702-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody - Azide and BSA Free [NBP3-03702] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Glutathione Synthetase Rabbit pAb (A14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03702_rabbit-polyclonal-glutathione-synthetase-antibody-52202519313410.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody - Azide and BSA Free [NBP3-03702] - Immunofluorescence analysis of U2OS cells using [KO Validated] Glutathione Synthetase Rabbit pAb (A14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03702_rabbit-polyclonal-glutathione-synthetase-antibody-52202519302977.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody - Azide and BSA Free [NBP3-03702] - Immunofluorescence analysis of PC-12 cells using [KO Validated] Glutathione Synthetase Rabbit pAb (A14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03702_rabbit-polyclonal-glutathione-synthetase-antibody-5220251931397.jpg)
![Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Human Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Western-Blot-NBP3-18258-img0003.jpg "Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Human Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) alpha-Synuclein Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Immunocytochemistry-Immunofluorescence-NBP3-18258-img0001.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Tissue: cerebellum. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) alpha-Synuclein Antibody (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Immunohistochemistry-NBP3-18258-img0002.jpg)
![Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Mouse, Rat Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Western-Blot-NBP3-18258-img0004.jpg)
![Western Blot: PrPC Antibody [NB100-56605] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 162-170 and (B) fusion partner without these amino acids, using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-Western-Blot-NB100-56605-img0002.jpg "Western Blot: PrPC Antibody [NB100-56605] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 162-170 and (B) fusion partner without these amino acids, using this antibody at 5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: PrPC Antibody [NBP2-38508] - Staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38508-img0003.jpg "Immunocytochemistry/Immunofluorescence: PrPC Antibody [NBP2-38508] - Staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/PrPC-Antibody-Immunohistochemistry-Paraffin-NBP2-38508-img0007.jpg)
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-Immunohistochemistry-NBP2-38508-img0002.jpg)
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human cerebral cortex shows strong granuclar cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/PrPC-Antibody-Immunohistochemistry-Paraffin-NBP2-38508-img0005.jpg)
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PrPC-Antibody-Immunohistochemistry-Paraffin-NBP2-38508-img0006.jpg)