| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33076 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-46853SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47911-0.1mg | |||
| IHC-Prediluted | NBP2-48363 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58869-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40775SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21180-25ul | |||
| Azide and BSA Free | NBP3-44262 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81817-25ul | |||
![Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0005.jpg "Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-326). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-326-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0002.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of GAPDH in nu/nu Mouse cells. 5% BSA in PBS, 1:5,000. Secondary: Goat anti-Rabbit IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0003.jpg)
![Simple Western: GAPDH Antibody [NB300-326] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-326-img0001.jpg)
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0002.jpg "Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.")
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0001.jpg)
![Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Western-Blot-NBP2-27117-img0008.jpg "Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis of decalcified, FFPE tissue sections from the paws of mice with collagen-induced arthritis using the HMGB1 antibody. The antibody was used at 5 ug/ml. Data courtesy of Dr Ulf Andersson, Karolinska Institute.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27117-img0007.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody [NBP2-27117] - Intracellular analysis using HMGB1 antibody. Human monocytes were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-27117 and APC secondary this antibody were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Flow-Cytometry-NBP2-27117-img0006.jpg)
![Simple Western: HMGB1/HMG-1 Antibody [NBP2-27117] - Lane view shows a specific band for HMGB1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Simple-Western-NBP2-27117-img0005.jpg)
![Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46853-img0003.jpg "Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain")
![Immunohistochemistry: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN1 in human fetal brain](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-NBP1-46853-img0004.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16590] - Immunohistochemical analysis of paraffin-embedded Gastric carcinoma, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16590-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: A549 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0001.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysate B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0002.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - A. 50 ug rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16590] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0005.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16590] - Sandwich ELISA detection of recombinant full-length Galectin 3 protein using as detection antibody at concentration of 1 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16590-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)")
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - KG-1 cells using CD34 Monoclonal Antibody (HPCA1/1171) (red) & isotype control (green).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Flow-Cytometry-NBP2-44569-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0002.jpg)
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Percent of EPCs (CD133 + CD34 +) reduced in the XYK group detected by flow cytometry. Data are mean +/- SD analyzed by one-way analysis of variance. **P < 0.01. CSDH, chronic subdural hematoma; EPC, endothelial progenitor cell; XYK, Xiaoyukang Jiaonang. PE conjugated CD34 antibody used (NBP2-47911PE). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32328124/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-44569_mouse-monoclonal-cd34-antibody-hpca1-1171-177202495947.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869] - Analysis of tissue lysates using mouse mAb to UCHL1, NB110-58869, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. The single band at 24kDa corresponds to the UCHL1 protein.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-BH7-Western-Blot-NB110-58869-img0006.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869] - Analysis of tissue lysates using mouse mAb to UCHL1, NB110-58869, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. The single band at 24kDa corresponds to the UCHL1 protein.")
![Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody (BH7) [NB110-58869] - Shows human embryonic kidney cell line 293 stained with mouse monoclonal to UCHL1 (NB110-58869) (green) and rabbit antibody to neurofilament NF-M, (NB300-133), (red). Blue is a DNA stain. Note that some of the Hek293 cells, which perhaps surprisingly have some properties of neuronal lineage cells (4), are beginning to express both UCHL1 and NF-M, both markers of neurons. The majority of cells, however express neither protein. Note that the UCHL1 is cytoplasmic and diffuse while the NF-M is concentrated in cytoplasmic filaments.](http://images.novusbio.com/fullsize/PGP9-5-UCHL-1-Antibody-BH7-Immunocytochemistry-Immunofluorescence-NB110-58869-img0002.jpg)
![Immunohistochemistry Free-Floating: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869] - Analysis of rat hippocampal section stained with mouse mAb to UCHL1, NB110-58869, dilution 1:5,000 in green and costained with rabbit pAb to FOX/NeuN, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. The UCHL1 antibody stains the cell body and dendrites of hippocampal neurons, while the FOX3 antibody labels nuclei of the neuronal cells.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-BH7-Immunocytochemistry-Immunofluorescence-NB110-58869-img0005.jpg)
![Simple Western: PGP9.5 / UCHL-1 Antibody (BH7) [NB110-58869] - Simple Western lane view shows a specific band for PGP9.5/UCHL-1 in 0.02 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGP9-5-UCHL-1-Antibody-BH7-Simple-Western-NB110-58869-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40775] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/DISC1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40775-img0004.jpg "Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40775] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).")
![Immunohistochemistry: DISC1 Antibody [NB110-40775] - Staining of DISC1 in mouse brain neurons, glial cells, and white matter. 40X](http://images.novusbio.com/fullsize/DISC1-Antibody-Immunohistochemistry-NB110-40775-img0003.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Western-Blot-NBP2-19952-img0004.jpg "Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP2-19952] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19952-img0002.jpg)
![Western Blot: Proteasome 19S S5A Antibody [NBP2-19952] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-19952-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 was knocked down in HCT116 cells by four different siRNAs and the cell lysates immunoprobed as indicated. Mock and scrambled control samples are included. b-actin is used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Knockdown-Validated-NBP2-19952-img0003.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241537198.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241538418.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202415291972.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113548.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113543.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241611358.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416121996.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - A C-terminal domain in hRpn10 binds E6AP AZUL.a Positions of known functional domains within hRpn10 (top) & E6AP isoform II (bottom). Question mark “?” indicates hRpn10 uncharacterized region & the E6AP catalytic cysteine C843 is indicated. b Pull-down assay of His-tagged hRpn10full-length (full), hRpn10196–377 or hRpn10196–306 without (−) or with (+) incubation of E6AP. c1H, 15N HSQC spectra of 0.2 mM 15N-hRpn10305–377 (black) & with twofold molar excess unlabeled AZUL (green). Shifted signals are labeled. d Table summarizing Kd, kon, & koff average values with standard deviations for the hRpn10305–377: AZUL interaction measured by ITC and/or SPR. N/A, not applicable. e HCT116 lysates expressing empty vector, myc-hRpn10 full length, or myc-Rpn10 with RAZUL deleted ( delta 307–377) were subjected to myc-immunoprecipitation with anti-myc-tag nanobody-coupled agarose. Whole cell extracts (WCE) & myc-immunoprecipitates were immunoprobed with the indicated antibodies. Cyclophilin B (Cyp B) is used as a loading control in e & f. f Lysates from HCT116 cells expressing HA-E6AP & the myc-hRpn10 constructs of e were subjected to HA IP followed by immunoblotting with the indicated antibodies. An asterisk “*” indicates non-specific interaction; double asterisk “**” indicates heavy chain antibody. e–f All antibodies used for immunoprobing are indicated to the left of the images. Note that the hRpn10 & E6AP antibodies recognize both endogenous & exogenously expressed protein, causing these panels to show both tagged & endogenous protein. g Schematic representation highlighting interaction domains of hRpn10 including newly identified RAZUL. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416114714.jpg)
![Western Blot: Coronin-1a Antibody (4G10) [H00011151-M01] - CORO1A monoclonal antibody (M01), clone 4G10 Analysis of CORO1A expression in K-562.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Western-Blot-H00011151-M01-img0007.jpg "Western Blot: Coronin-1a Antibody (4G10) [H00011151-M01] - CORO1A monoclonal antibody (M01), clone 4G10 Analysis of CORO1A expression in K-562.")
![Immunohistochemistry-Paraffin: Coronin-1a Antibody (4G10) [H00011151-M01] - Analysis of monoclonal antibody to CORO1A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Immunohistochemistry-Paraffin-H00011151-M01-img0005.jpg)
![Sandwich ELISA: Coronin-1a Antibody (4G10) [H00011151-M01] - Detection limit for recombinant GST tagged CORO1A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Sandwich-ELISA-H00011151-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining in human cerebral cortex and skeletal muscle tissues using anti-MAG antibody. Corresponding MAG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP1-81817-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining in human cerebral cortex and skeletal muscle tissues using anti-MAG antibody. Corresponding MAG RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP1-81817-img0006.jpg)
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - MAG Antibody [NBP1-81817] - Image submitted by a verified customer review on mouse inner ear at 1:200 dilution.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-NBP1-81817-img0004.jpg)
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP1-81817-img0005.jpg)