| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12602-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98717-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89982-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50303-0.025ml | |||
![Western Blot: PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) [NBP3-16930] - Western blot analysis of extracts of various cell lines, using PAK1/2/3 Rabbit mAb (NBP3-16930) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PAK1-2-3-p-Ser141-p-Ser139-p-Ser144-Antibody-1T7A5-Western-Blot-NBP3-16930-img0001.jpg "Western Blot: PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) [NBP3-16930] - Western blot analysis of extracts of various cell lines, using PAK1/2/3 Rabbit mAb (NBP3-16930) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511] - Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/PAK1-[p-Thr84]-Antibody-Western-Blot-NBP2-29511-img0002.jpg "Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511] - Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/FANCI-[p-Ser556]-Antibody-Western-Blot-NBP2-60780-img0004.jpg "Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")
![Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of HeLa cell lysates that had been treated withUV (~254 nm) for 0' or 20' showing specific immunolabeling of the~150 kDa FANCI protein phosphorylated at Ser559.](http://images.novusbio.com/fullsize/FANCI-[p-Ser556]-Antibody-Western-Blot-NBP2-60780-img0002.jpg)
![Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/FANCI-[p-Ser559]-Antibody-Western-Blot-NBP2-60781-img0003.jpg "Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")
![Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of HeLa cell lysates that had been treated withUV (~254 nm) for 0' or 20' showing specific immunolabeling of the~150 kDa FANCI protein phosphorylated at Ser559.](http://images.novusbio.com/fullsize/FANCI-[p-Ser559]-Antibody-Western-Blot-NBP2-60781-img0001.jpg)
![Western Blot: PLK5 Antibody [NBP3-12602] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PLK5-Antibody-Western-Blot-NBP3-12602-img0001.jpg "Western Blot: PLK5 Antibody [NBP3-12602] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: PLK5 Antibody [NBP3-12602] - Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-PLK5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PLK5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size :36 kDa](http://images.novusbio.com/fullsize/PLK5-Antibody-Immunoprecipitation-NBP3-12602-img0002.jpg)
![Western Blot: Numb Antibody [NBP2-98717] - Anti-Numb rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/Numb-Antibody-Western-Blot-NBP2-98717-img0002.jpg "Western Blot: Numb Antibody [NBP2-98717] - Anti-Numb rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 70 kDa")
![Immunoprecipitation: Numb Antibody [NBP2-98717] - Numb was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-Numb rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-Numb rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/Numb-Antibody-Immunoprecipitation-NBP2-98717-img0001.jpg)
![Western Blot: Numb Antibody [NBP3-30927] - Western blot using anti-Numb antibody (Cat. No. NBP3-30927) on human brain lysate (14ug/lane). Antibody was used with (lane 1) and without (lane 2) blocking peptide at 10ug/ml.](http://images.novusbio.com/fullsize/nbp3-30927_rabbit-numb-pab-81120242059219.jpg "Western Blot: Numb Antibody [NBP3-30927] - Western blot using anti-Numb antibody (Cat. No. NBP3-30927) on human brain lysate (14ug/lane). Antibody was used with (lane 1) and without (lane 2) blocking peptide at 10ug/ml.")
![Immunohistochemistry-Paraffin: BAI1 Antibody - BSA Free [NB100-59011] - Analysis of anti-BAI1 antibody with human prostate.](http://images.novusbio.com/fullsize/BAI1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59011-img0002.jpg "Immunohistochemistry-Paraffin: BAI1 Antibody - BSA Free [NB100-59011] - Analysis of anti-BAI1 antibody with human prostate.")
![Flow Cytometry: CALML5 Antibody (158) [NBP2-89982] - Analysis of Human CALML5 expression on A431 cells. The cells were treated, stained with purified anti-Human CALML5, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CALML5-Antibody-158-Flow-Cytometry-NBP2-89982-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: CALML5 Antibody (158) [NBP2-89982] - Analysis of Human CALML5 expression on A431 cells. The cells were treated, stained with purified anti-Human CALML5, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CALML5 Antibody (158) [NBP2-89982] - Staining of Human CALML5 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89982 antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89982_rabbit-monoclonal-calml5-antibody-158-23520251125443.png)
![Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.](http://images.novusbio.com/fullsize/nbp3-23345_rabbit-profilin-1-p-tyr129-pab-19920231856966.jpg "Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.")
![Western Blot: CENPJ Antibody [NBP1-50018] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used at 0.04 ug/ml.](http://images.novusbio.com/fullsize/CENPJ-Antibody-Western-Blot-NBP1-50018-img0002.jpg "Western Blot: CENPJ Antibody [NBP1-50018] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used at 0.04 ug/ml.")