| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54748-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76786-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76785-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38548-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03729-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94571-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22411-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37475-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21437-25ul | |||
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-54748] - Analysis in human cerebral cortex and pancreas tissues using Anti-GFAP antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-54748-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-54748] - Analysis in human cerebral cortex and pancreas tissues using Anti-GFAP antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-54748] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-54748-img0006.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-54748] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-54748-img0005.jpg)
![Western Blot: NPAS3 Antibody [NBP1-76786] - Rat brain tissue lysate with NPAS3 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/NPAS3-Antibody-Western-Blot-NBP1-76786-img0001.jpg "Western Blot: NPAS3 Antibody [NBP1-76786] - Rat brain tissue lysate with NPAS3 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.")
![Immunohistochemistry-Paraffin: NPAS3 Antibody [NBP1-76786] - Human brain tissue with NPAS3 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/NPAS3-Antibody-Immunohistochemistry-Paraffin-NBP1-76786-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: NPAS3 Antibody - BSA Free [NBP1-76786] - Immunofluorescence of NPAS3 in Human Brain cells with NPAS3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76786_rabbit-polyclonal-npas3-antibody-2711202419251543.jpg)
![Western Blot: NPAS3 Antibody [NBP1-76785] - SK-N-SH cell lysate with NPAS3 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/NPAS3-Antibody-Western-Blot-NBP1-76785-img0001.jpg "Western Blot: NPAS3 Antibody [NBP1-76785] - SK-N-SH cell lysate with NPAS3 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.")
![Immunohistochemistry-Paraffin: NPAS3 Antibody [NBP1-76785] - Human brain tissue with NPAS3 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/NPAS3-Antibody-Immunohistochemistry-Paraffin-NBP1-76785-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: NPAS3 Antibody - BSA Free [NBP1-76785] - Immunofluorescence of NPAS3 in human brain tissue with NPAS3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76785_rabbit-polyclonal-npas3-antibody-27112024200410.jpg)
![Western Blot: MAO-A Antibody [NB100-2417] - Analysis of MAO-A in Human Heart lysate using this antibody at 0.3 ug/mL with (B) and without (A) blocking with the immunising peptide. Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Western-Blot-NB100-2417-img0007.jpg "Western Blot: MAO-A Antibody [NB100-2417] - Analysis of MAO-A in Human Heart lysate using this antibody at 0.3 ug/mL with (B) and without (A) blocking with the immunising peptide. Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: MAO-A Antibody [NB100-2417] - Staining of paraffin embedded Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Immunohistochemistry-Paraffin-NB100-2417-img0009.jpg)
![Immunohistochemistry-Paraffin: MAO-A Antibody [NB100-2417] - Staining of paraffin embedded Human Placenta. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Immunohistochemistry-Paraffin-NB100-2417-img0008.jpg)
![Western Blot: Plakophilin 2 Antibody [NBP1-72054] - (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Western-Blot-NBP1-72054-img0002.jpg "Western Blot: Plakophilin 2 Antibody [NBP1-72054] - (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-72054] - 3.75ug/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-72054-img0003.jpg)
![Western Blot: TAF15 Antibody [NBP3-38548] - Western blot analysis of various lysates using TAF15 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38548_rabbit-taf15-pab-912202415143693.jpg "Western Blot: TAF15 Antibody [NBP3-38548] - Western blot analysis of various lysates using TAF15 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5s.")
![Immunoprecipitation: TAF15 Antibody [NBP3-38548] - Immunoprecipitation analysis of 200 ug extracts of SKOV3 cells using 3 ug TAF15 antibody. Western blot was performed from the immunoprecipitate using TAF15 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38548_rabbit-taf15-pab-912202414201367.jpg)
![Immunohistochemistry: TAF15 Antibody [NBP3-38548] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using TAF15 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38548_rabbit-taf15-pab-912202414154393.jpg)
![Immunocytochemistry/ Immunofluorescence: Kv1.1 Antibody - Azide and BSA Free [Kv1.1] - Immunofluorescence analysis of paraffin-embedded mouse brain using Kv1.1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03729_rabbit-polyclonal-kv1-1-antibody-5220251639619.jpg "Immunocytochemistry/ Immunofluorescence: Kv1.1 Antibody - Azide and BSA Free [Kv1.1] - Immunofluorescence analysis of paraffin-embedded mouse brain using Kv1.1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: Kv1.1 Antibody - Azide and BSA Free [Kv1.1] - Western blot analysis of lysates from HeLa cells, using Kv1.1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-03729_rabbit-polyclonal-kv1-1-antibody-5220251639641.jpg)
![Western Blot: Kv1.1 Antibody - Azide and BSA Free [Kv1.1] - Western blot analysis of various lysates, using Kv1.1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-03729_rabbit-polyclonal-kv1-1-antibody-52202516321460.jpg)
![Western Blot: VGLUT2 Antibody [NBP2-94571] - Analysis of extracts of various cell lines, using VGLUT2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-Western-Blot-NBP2-94571-img0003.jpg "Western Blot: VGLUT2 Antibody [NBP2-94571] - Analysis of extracts of various cell lines, using VGLUT2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: VGLUT2 Antibody - Azide and BSA Free [NBP2-94571] - Immunofluorescence analysis of rat brain using VGLUT2 Rabbit pAb (A15177) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94571_rabbit-polyclonal-vglut2-antibody-52202519302916.jpg)
![Immunocytochemistry/ Immunofluorescence: VGLUT2 Antibody - Azide and BSA Free [NBP2-94571] - Immunofluorescence analysis of mouse brain using VGLUT2 Rabbit pAb (A15177) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94571_rabbit-polyclonal-vglut2-antibody-52202519294594.jpg)
![Western Blot: Stargazin Antibody (S245-36) [NBP2-22411] - Western Blot analysis of Rat brain lysates showing detection of StargazinCalcium Channel protein using Mouse Anti-StargazinCalcium Channel Monoclonal Antibody, Clone S245-36 (NBP2-22411). Primary Antibody: Mouse Anti-StargazinCalcium Channel Monoclonal Antibody (NBP2-22411) at 1:100, 1:250, 1:500, and 1:1000.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Western-Blot-NBP2-22411-img0003.jpg "Western Blot: Stargazin Antibody (S245-36) [NBP2-22411] - Western Blot analysis of Rat brain lysates showing detection of StargazinCalcium Channel protein using Mouse Anti-StargazinCalcium Channel Monoclonal Antibody, Clone S245-36 (NBP2-22411). Primary Antibody: Mouse Anti-StargazinCalcium Channel Monoclonal Antibody (NBP2-22411) at 1:100, 1:250, 1:500, and 1:1000.")
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (NBP2-22411). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (NBP2-22411) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (NBP2-22411). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (NBP2-22411) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Stargazin-Antibody-S245-36-Immunocytochemistry-Immunofluorescence-NBP2-22411-img0005.jpg)
![Western Blot: GluR2 Antibody (7G6) [NBP2-37475] - Analysis using GRIA2 mouse mAb against HeLa (1) cell lysate.](http://images.novusbio.com/fullsize/GluR2-Antibody-7G6-Western-Blot-NBP2-37475-img0004.jpg "Western Blot: GluR2 Antibody (7G6) [NBP2-37475] - Analysis using GRIA2 mouse mAb against HeLa (1) cell lysate.")
![Flow Cytometry: GluR2 Antibody (7G6) [NBP2-37475] - Analysis of SK-N-SH cells using GRIA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GluR2-Antibody-7G6-Flow-Cytometry-NBP2-37475-img0005.jpg)
![Western Blot: GluR2 Antibody (7G6) [NBP2-37475] - Analysis using GRIA2 mAb against human GRIA2 recombinant protein. (Expected MW is 43 kDa)](http://images.novusbio.com/fullsize/GluR2-Antibody-7G6-Western-Blot-NBP2-37475-img0002.jpg)
![Western Blot: GluR2 Antibody (7G6) [NBP2-37475] - Analysis using GRIA2 mAb against HEK293 (1) and GRIA2 (AA: 652-807)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GluR2-Antibody-7G6-Western-Blot-NBP2-37475-img0003.jpg)
![ELISA: GluR2 Antibody (7G6) [NBP2-37475] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/GluR2-Antibody-7G6-ELISA-NBP2-37475-img0001.jpg)
![Western Blot: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Western blot analysis of Pannexin-1 on different lysates with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C2C12 cell lysate (20 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32714) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-278202415212479.jpg "Western Blot: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Western blot analysis of Pannexin-1 on different lysates with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C2C12 cell lysate (20 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32714) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32714) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-298202413585529.jpg)
![Immunocytochemistry/ Immunofluorescence: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Immunocytochemistry analysis of 293T cells labeling Pannexin-1 with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-292024111486.jpg)
![Immunoprecipitation: CoCoA Antibody [NBP3-21437] - CoCoA was immunoprecipitated using: Lane A:0.5 mg NIH3T3 Whole Cell Lysate 4 µL NBP3-21437 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-21437,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 77 kDa Observed band size :100 kDa](http://images.novusbio.com/fullsize/nbp3-21437_rabbit-cocoa-pab-136202422345084.png)
![Immunocytochemistry/ Immunofluorescence: CoCoA Antibody [NBP3-21437] - Immunofluorescence staining of CoCoA in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-21437 (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-21437_rabbit-cocoa-pab-235202514492329.png)