| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34512-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47959-0.1mg | |||
| IHC-Prediluted | NBP2-48463 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78393SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44855 | |||
| Azide and BSA Free | NBP2-34708-0.1mg | |||
![Western Blot: Kir6.2 Antibody [NBP2-76944] - Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human liver tissue lysateLane 2: Mouse liver tissue lysate](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Western-Blot-NBP2-76944-img0001.jpg "Western Blot: Kir6.2 Antibody [NBP2-76944] - Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human liver tissue lysateLane 2: Mouse liver tissue lysate")
![Immunocytochemistry/Immunofluorescence: Kir6.2 Antibody [NBP2-76944] - ICC staining of Kir6.2 in LoVo cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76944-img0002.jpg)
![Immunohistochemistry: Kir6.2 Antibody [NBP2-76944] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunohistochemistry-NBP2-76944-img0005.jpg)
![Immunohistochemistry: Kir6.2 Antibody [NBP2-76944] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunohistochemistry-NBP2-76944-img0003.jpg)
![Immunohistochemistry: Kir6.2 Antibody [NBP2-76944] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunohistochemistry-NBP2-76944-img0004.jpg)
![Western Blot: Secretagogin Antibody (JE60-48) [NBP3-32933] - Western blot analysis of Secretagogin on different lysates with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. Lane 1: TT cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse eyeball tissue lysate Lane 4: Mouse pancreas tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32933) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-288202412461645.jpg "Western Blot: Secretagogin Antibody (JE60-48) [NBP3-32933] - Western blot analysis of Secretagogin on different lysates with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. Lane 1: TT cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse eyeball tissue lysate Lane 4: Mouse pancreas tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32933) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Secretagogin Antibody (JE60-48) [NBP3-32933] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32933) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-29820241420927.jpg)
![Immunocytochemistry/ Immunofluorescence: Secretagogin Antibody (JE60-48) [NBP3-32933] - Immunocytochemistry analysis of TT cells labeling Secretagogin with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-29202410334089.jpg)
![Flow Cytometry: Secretagogin Antibody (JE60-48) [NBP3-32933] - Flow cytometric analysis of TT cells labeling Secretagogin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32933, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-392024884687.jpg)
![Western Blot: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with HL60 cell line lysates (35ug/lane).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Western-Blot-NBP1-50529-img0001.jpg "Western Blot: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with HL60 cell line lysates (35ug/lane).")
![Immunocytochemistry/Immunofluorescence: LIN-28B Antibody [NBP1-50529] - Fluorescent confocal image of SY5Y cells stained with LIN28B antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LIN28B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LIN28B immunosignal to the cytoplasm.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-50529-img0008.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-50529] - Human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-50529-img0006.jpg)
![Flow Cytometry: LIN-28B Antibody [NBP1-50529] - Flow cytometric of HL-60 cells using LIN28B Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Flow-Cytometry-NBP1-50529-img0010.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with human spleen at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-50529-img0003.jpg)
![Western Blot: CD38 Antibody (PD01-49) [NBP3-32151] - Western blot analysis of CD38 on Daudi cell lysates with Rabbit anti-CD38 antibody (NBP3-32151) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-278202416285274.jpg "Western Blot: CD38 Antibody (PD01-49) [NBP3-32151] - Western blot analysis of CD38 on Daudi cell lysates with Rabbit anti-CD38 antibody (NBP3-32151) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD38 Antibody (PD01-49) [NBP3-32151] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD38 antibody (NBP3-32151) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32151) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-29820241435231.jpg)
![Immunocytochemistry/ Immunofluorescence: CD38 Antibody (PD01-49) [NBP3-32151] - Immunocytochemistry analysis of A549 cells labeling CD38 with Rabbit anti-CD38 antibody (NBP3-32151) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD38 antibody (NBP3-32151) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-2920241192330.jpg)
![Flow Cytometry: CD38 Antibody (PD01-49) [NBP3-32151] - Flow cytometric analysis of THP-1 cells labeling CD38. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32151, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-29202416453252.jpg)
![Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-288202412461658.jpg "Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32834) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-298202414333688.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunocytochemistry analysis of HepG2 cells labeling PMP70 with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-29202410334056.jpg)
![Flow Cytometry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Flow cytometric analysis of NIH/3T3 cells labeling PMP70. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32834, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-2920241637212.jpg)
![Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-288202412493458.jpg "Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32961) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-298202414161869.jpg)
![Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202410484855.jpg)
![Flow Cytometry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Flow cytometric analysis of SH-SY5Y cells labeling SNAP25. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32961, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202416372172.jpg)
![Western Blot: Tbp7 Antibody (3G8) [H00005704-M01] - Analysis of PSMC4 expression in transfected 293T cell line by PSMC4 monoclonal antibody (M01), clone 3G8.Lane 1: PSMC4 transfected lysate(47.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Tbp7-Antibody-3G8-Western-Blot-H00005704-M01-img0012.jpg "Western Blot: Tbp7 Antibody (3G8) [H00005704-M01] - Analysis of PSMC4 expression in transfected 293T cell line by PSMC4 monoclonal antibody (M01), clone 3G8.Lane 1: PSMC4 transfected lysate(47.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Tbp7 Antibody (3G8) [H00005704-M01] - Analysis of monoclonal antibody to PSMC4 on HepG2 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Tbp7-Antibody-3G8-Immunocytochemistry-Immunofluorescence-H00005704-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Tbp7 Antibody (3G8) [H00005704-M01] - Analysis of monoclonal antibody to PSMC4 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Tbp7-Antibody-3G8-Immunohistochemistry-Paraffin-H00005704-M01-img0009.jpg)
![Western Blot: Tbp7 Antibody (3G8) [H00005704-M01] - PSMC4 monoclonal antibody (M01), clone 3G8 Analysis of PSMC4 expression in HepG2.](http://images.novusbio.com/fullsize/Tbp7-Antibody-3G8-Western-Blot-H00005704-M01-img0011.jpg)
![Sandwich ELISA: Tbp7 Antibody (3G8) [H00005704-M01] - Detection limit for recombinant GST tagged PSMC4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Tbp7-Antibody-3G8-Sandwich-ELISA-H00005704-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: ACTH Antibody (57) - N-terminal [NBP2-32912] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using ACTH monoclonal antibody (57) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/ACTH-Antibody-57-N-terminal-Immunohistochemistry-Paraffin-NBP2-32912-img0002.jpg "Immunohistochemistry-Paraffin: ACTH Antibody (57) - N-terminal [NBP2-32912] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using ACTH monoclonal antibody (57) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: ACTH Antibody (57) - N-terminal [NBP2-32912] - Formalin-fixed, paraffin-embedded human Pituitary Gland stained with ACTH Monoclonal Antibody (57).](http://images.novusbio.com/fullsize/ACTH-Antibody-57-N-terminal-Immunohistochemistry-Paraffin-NBP2-32912-img0001.jpg)
![Immunohistochemistry-Paraffin: ACTH Antibody (57) - N-terminal [NBP2-32912] - Formalin-fixed, paraffin-embedded human pituitary gland stained with ACTH Mouse Monoclonal Antibody (57).](http://images.novusbio.com/fullsize/ACTH-Antibody-57-N-terminal-Immunohistochemistry-Paraffin-NBP2-32912-img0003.jpg)
![Western Blot: BMAL1 Antibody [NB100-129] - Detection of BMAL in 3T3/L1 lysates.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Western-Blot-NB100-129-img0003.jpg "Western Blot: BMAL1 Antibody [NB100-129] - Detection of BMAL in 3T3/L1 lysates.")
![Simple Western: BMAL1 Antibody [NB100-129] - Image shows a specific band for BMAL1 in 0.5 mg/mL of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Simple-Western-NB100-129-img0004.jpg)
![Western Blot: CD45RB Antibody (PTPRC/1147) [NBP2-44847] - Western Blot Analysis of human Spleen tissue lysates using CD45RB Antibody (PTPRC/1147).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PTPRC-1147-Western-Blot-NBP2-44847-img0004.jpg "Western Blot: CD45RB Antibody (PTPRC/1147) [NBP2-44847] - Western Blot Analysis of human Spleen tissue lysates using CD45RB Antibody (PTPRC/1147).")
![Immunohistochemistry-Paraffin: CD45RB Antibody (PTPRC/1147) [NBP2-44847] - Human Tonsil stained with CD45RB Monoclonal Antibody (PTPRC/1147).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PTPRC-1147-Immunohistochemistry-Paraffin-NBP2-44847-img0002.jpg)
![Western Blot: CD45RB Antibody (PTPRC/1147) [NBP2-44847] - Analysis of Daudi Cell Lysate using CD45RB Monoclonal Antibody (PTPRC/1147).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PTPRC-1147-Western-Blot-NBP2-44847-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45RB Antibody (PTPRC/1147) [NBP2-44847] - Human Lymphoma stained with CD45RB Monoclonal Antibody (PTPRC/1147).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PTPRC-1147-Immunohistochemistry-Paraffin-NBP2-44847-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SERF1A Antibody [NBP1-78393] - Serf1a antibody was tested in Ntera cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SERF1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78393-img0005.jpg "Immunocytochemistry/Immunofluorescence: SERF1A Antibody [NBP1-78393] - Serf1a antibody was tested in Ntera cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: SERF1A Antibody [NBP1-78393] - Analysis of SERF1A in human Alzheimer's brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/SERF1A-Antibody-Immunohistochemistry-NBP1-78393-img0003.jpg)
![Western Blot: CD45RB Antibody (PD7/26) [NBP2-32937] - Western Blot Analysis of human Spleen tissue lysates using CD45RB Antibody (PD7/26).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PD7-26-Western-Blot-NBP2-32937-img0005.jpg "Western Blot: CD45RB Antibody (PD7/26) [NBP2-32937] - Western Blot Analysis of human Spleen tissue lysates using CD45RB Antibody (PD7/26).")
![Immunohistochemistry-Paraffin: CD45RB Antibody (PD7/26) [NBP2-32937] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD45RB MAb (PD7/26).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PD7-26-Immunohistochemistry-Paraffin-NBP2-32937-img0002.jpg)
![Flow Cytometry: CD45RB Antibody (PD7/26) [NBP2-32937] - Flow Cytometric Analysis of PFA-fixed Raji cells using CD45RB Antibody (PD7/26) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PD7-26-Flow-Cytometry-NBP2-32937-img0004.jpg)
![Immunohistochemistry-Paraffin: CD45RB Antibody (PD7/26) [NBP2-32937] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD45RB MAb (PD7/26).](http://images.novusbio.com/fullsize/nbp2-32937_mouse-monoclonal-cd45rb-antibody-pd7-26-17720249362610.jpg)