| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78299SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-136-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50612-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38258-20ul | |||
![Western Blot: PSENEN/PEN2 Antibody [NBP2-24611] - Analysis of Presenilin enhancer protein 2 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse intestine lysate using NBP2-24611. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-Western-Blot-NBP2-24611-img0002.jpg "Western Blot: PSENEN/PEN2 Antibody [NBP2-24611] - Analysis of Presenilin enhancer protein 2 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse intestine lysate using NBP2-24611. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NBP1-78299] - LRRK2 antibody was tested at 1:100 in U2OS cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78299-img0002.jpg "Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NBP1-78299] - LRRK2 antibody was tested at 1:100 in U2OS cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: LRRK2 Antibody [NBP1-78299] - Analysis of LRRK2 in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunohistochemistry-NBP1-78299-img0003.jpg)
![Western Blot: NF-H Antibody (NAP4) [NB300-136] - Analysis of tissue lysates using mouse mAb to NF-H, NB300-136, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord, [7] cow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with NB300-136 antibody.](http://images.novusbio.com/fullsize/NF-H-Antibody-NAP4-Western-Blot-NB300-136-img0002.jpg "Western Blot: NF-H Antibody (NAP4) [NB300-136] - Analysis of tissue lysates using mouse mAb to NF-H, NB300-136, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord, [7] cow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with NB300-136 antibody.")
![Immunohistochemistry: NF-H Antibody (NAP4) [NB300-136] - Analysis of human cerebellar cortex section stained with mouse mAb to pNF-H, NB300-136, in brown. Paraffin-embedded, formalin-fixed tissue sections were stained with this antibody using the avidin biotin conjugate method. The sections was counterstained with Hematoxylin in blue. NB300-136 stains prominent basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image, the molecular layer at the top.](http://images.novusbio.com/fullsize/NF-H-Antibody-NAP4-Immunohistochemistry-NB300-136-img0001.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0011.jpg "Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32083-img0008.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody diluted by 1:1000](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0006.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. 293T mock transfected (-) or transfected with a construct expressing human Ataxin 3. 10 % SDS-PAGE Ataxin 3 antibody dilution: 1:20000](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32083-img0007.jpg)
![Western Blot: BACE2 Antibody [NBP2-15539] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/BACE2-Antibody-Western-Blot-NBP2-15539-img0001.jpg "Western Blot: BACE2 Antibody [NBP2-15539] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: BACE2 Antibody [NBP2-15539] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BACE2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15539-img0002.jpg)
![Immunohistochemistry-Paraffin: BACE-2 Antibody [NBP2-15539] - : Paraffin-embedded human breast carcinoma. BACE2 antibody [C1C3] dilution: 1:500.](http://images.novusbio.com/fullsize/BACE-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15539-img0005.jpg)
![Immunohistochemistry-Paraffin: BACE-2 Antibody [NBP2-15539] - Paraffin-embedded Rat brain. BACE2 antibody [C1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/BACE-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15539-img0003.jpg)
![Immunohistochemistry-Paraffin: BACE-2 Antibody [NBP2-15539] - Paraffin-embedded rat fore brain. BACE2 antibody [C1C3] dilution: 1:500.](http://images.novusbio.com/fullsize/BACE-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15539-img0004.jpg)
![Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NBP2-50612-img0002.jpg "Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP2-50612] - Immunofluorescent analysis of a section of mouse cerebellum stained with NBP2-50612 in green, and co-stained with a chicken polyclonal antibody to FOX2 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NF-L antibody labels dendrites and axons of neuronal cells predominantly axons in white matter and basket cell axons associated with Purkinje cells. The FOX2 antibody reveals protein expressed in a subset of neurons, including the Purkinje cells.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50612-img0001.jpg)
![Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - LSM image showing strong expression of 5-HT6 (red) colocalized with virally transduced (green) TH (blue)-positive neurons within the dopaminergic fetal graft. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-HT6-Antibody-Immunohistochemistry-NBP1-46557-img0002.jpg "Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - LSM image showing strong expression of 5-HT6 (red) colocalized with virally transduced (green) TH (blue)-positive neurons within the dopaminergic fetal graft. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.")
![Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - The 5-HT6 Receptor Is highly expressed in human tissue grafts originating from both fetal and hESC. Immunofluorescence staining of 5-HT6 expression in a human dopaminergic fetal graft within the rat striatum with the phenotypic staining for TH , and the identification of human NCAM, imaged using LSM. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-HT6-Antibody-Immunohistochemistry-NBP1-46557-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: 5-HT6 Antibody [NBP1-46557] - The 5-HT6 Receptor Is Highly Expressed in Human Tissue Grafts Originating from Both Fetal & hESC Sources(A–E) Immunofluorescence staining of 5-HT6 expression in a human dopaminergic fetal graft within the rat striatum (A & C) with the phenotypic staining for TH (A & B), & the identification of human NCAM (A & D), imaged using LSM. The 5-HT6 expression was confirmed to reside in DA neurons originating from the transplanted tissue (E).(F–J) Similarly, immunofluorescence staining of a paraffin-embedded section originating from the putamen of a PD patient that received a dopaminergic fetal graft, imaged using LSM, confirmed that the 5-HT6 receptor (F & H) is highly expressed in the TH-positive (F & G, with overlay in J) DA neurons originating from the fetal graft. Nuclear staining (DAPI) confirmed the 5-HT6 expression to be abundant in the neuronal soma (I).(K–O) To confirm if this receptor is also abundant in DA neurons differentiated from hESCs, a graft derived from the H9 hESC line, differentiated using the novel floor-plate-based protocol & transplanted to the parkinsonian striatum of a nude rat, was evaluated using LSM with immunofluorescence for the same genes: 5-HT6 (K & M), TH (K & L), & the human NCAM (K & N). This confirmed that this receptor is highly expressed in DA neurons also differentiated from this cell source, when transplanted to the striatum. Overlay of all three antigens confirmed the 5-HT6 receptor to reside in hESC-derived DA neurons (O). Scale bar, 100 μm (K) & 50 μm (N). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27161524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-46557_rabbit-polyclonal-5-ht6-antibody-310202416171411.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lano Antibody [NBP1-88016] - Staining of human rectum shows high expression.](http://images.novusbio.com/fullsize/Lano-Antibody-Immunohistochemistry-Paraffin-NBP1-88016-img0013.jpg)
![Immunohistochemistry-Paraffin: Lano Antibody [NBP1-88016] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Lano-Antibody-Immunohistochemistry-Paraffin-NBP1-88016-img0012.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-88016_rabbit-polyclonal-lano-antibody-65202516254511.jpg)
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-2120249393566.jpg "Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at cell membrane by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:100. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-212024941475.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-2120249415931.jpg)
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Boiled and unboiled mouse tissue extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-212024943286.jpg)
![Immunohistochemistry: Recoverin Antibody [NBP3-38258] - Immunohistochemistry analysis of paraffin-embedded Mouse retina using Recoverin Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38258_rabbit-recoverin-pab-912202414163065.jpg "Immunohistochemistry: Recoverin Antibody [NBP3-38258] - Immunohistochemistry analysis of paraffin-embedded Mouse retina using Recoverin Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Recoverin Antibody [NBP3-38258] - Immunohistochemistry analysis of paraffin-embedded Rat retina using Recoverin Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38258_rabbit-recoverin-pab-912202415161810.jpg)
![Western Blot: Recoverin Antibody [NBP3-38258] - Western blot analysis of various lysates using Recoverin Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-38258_rabbit-recoverin-pab-9122024159535.jpg)
![Immunocytochemistry/ Immunofluorescence: Recoverin Antibody [NBP3-38258] - Immunofluorescence analysis of paraffin-embedded rat eye using Recoverin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38258_rabbit-recoverin-pab-912202414123816.jpg)
![Immunocytochemistry/ Immunofluorescence: Recoverin Antibody [NBP3-38258] - Immunofluorescence analysis of paraffin-embedded mouse eye using Recoverin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38258_rabbit-recoverin-pab-91220241519545.jpg)
![Western Blot: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Western blot analysis of extracts of Mouse brain, using Glutamate Receptor 6 Rabbit mAb (NBP3-16867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Glutamate-Receptor-6-Antibody-4B4L9-Western-Blot-NBP3-16867-img0004.jpg "Western Blot: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Western blot analysis of extracts of Mouse brain, using Glutamate Receptor 6 Rabbit mAb (NBP3-16867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Western blot analysis of extracts of 293T cells, using Glutamate Receptor 6 Rabbit mAb (NBP3-16867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Glutamate-Receptor-6-Antibody-4B4L9-Western-Blot-NBP3-16867-img0005.jpg)
![Immunohistochemistry: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Immunohistochemistry analysis of Glutamate Receptor 6 in paraffin-embedded human colon carcinoma tissue using Glutamate Receptor 6 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16867_rabbit-glutamate-receptor-6-mab-4b4l9-822025201041.jpg)
![Immunohistochemistry: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Immunohistochemistry analysis of Glutamate Receptor 6 in paraffin-embedded human kidney tissue using Glutamate Receptor 6 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16867_rabbit-glutamate-receptor-6-mab-4b4l9-822025208497.jpg)
![Immunohistochemistry: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Immunohistochemistry analysis of Glutamate Receptor 6 in paraffin-embedded mouse intestin tissue using Glutamate Receptor 6 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16867_rabbit-glutamate-receptor-6-mab-4b4l9-822025209849.jpg)
![Immunohistochemistry: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Immunohistochemistry analysis of Glutamate Receptor 6 in paraffin-embedded rat kidney tissue using Glutamate Receptor 6 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16867_rabbit-glutamate-receptor-6-mab-4b4l9-822025208480.jpg)
![Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-912202414154378.jpg "Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-31120251744416.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-31120251704787.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-3112025173144.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Human lung cancer tissue using Aquaporin-4 (AQP4) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-immunohistochemistry-paraffin-1352025122932..png)