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![Western Blot: Frizzled-8 Antibody [NB100-2439] - Staining of human pancreas lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Frizzled-8-Antibody-Western-Blot-NB100-2439-img0004.jpg "Western Blot: Frizzled-8 Antibody [NB100-2439] - Staining of human pancreas lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Frizzled-8 Antibody [NB100-2439] - Staining of paraffin embedded Human Pancreas. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-detection.](http://images.novusbio.com/fullsize/Frizzled-8-Antibody-Immunohistochemistry-Paraffin-NB100-2439-img0001.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Western-Blot-NBP2-95093-img0004.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded mouse stomach using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0003.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded human colon carcinoma using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded human gastric cancer using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0002.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Western blot analysis of various lysates using Sodium Potassium ATPase Beta 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-52202516345269.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Immunofluorescence analysis of NIH/3T3 cells using Sodium Potassium ATPase Beta 1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-5220251638037.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Immunofluorescence analysis of C6 cells using Sodium Potassium ATPase Beta 1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-5220251628041.jpg)
![Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Western-Blot-NBP3-16840-img0005.jpg "Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of mouse eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0004.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Rat retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0001.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Mouse retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0002.jpg)
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of rat eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of paraffin-embedded rat eye using RPE65 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16840_rabbit-rpe65-mab-5x1f6-52202511361295.jpg)
![Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - The figure depicts Western blots of the two synaptic plasticity markers investigated. The two bands seen in the p-synapsin and synapsin Western blots (70 and 74 kDa respectively) are consistent with splice variants. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/10/6/749) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Synapsin-I-[p-Ser603]-Antibody-Western-Blot-NB300-181-img0002.jpg "Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - The figure depicts Western blots of the two synaptic plasticity markers investigated. The two bands seen in the p-synapsin and synapsin Western blots (70 and 74 kDa respectively) are consistent with splice variants. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/10/6/749) licensed under a CC-BY license.")
![Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - Rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (l-Ptase, 1200 units for 30 minutes).](http://images.novusbio.com/fullsize/Synapsin-I-[p-Ser603]-Antibody-Western-Blot-NB300-181-img0001.jpg)
![Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - Examples of Western blots of p-/synapsin 1 & p-/Ca2+-calmodulin-dependent kinase II. The figure depicts examples of the Western blots of the four synaptic plasticity markers investigated. The two bands seen in the p-synapsin & synapsin Western blots (70 & 74 kDa respectively) are consistent with splice variants as confirmed by manufacturer. P-CAMKII & CAMKII were detected around 50 kDa in accordance with previous findings. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29890692), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-181_rabbit-polyclonal-synapsin-i-p-ser603-antibody-imgenex-img-683-31020241539736.jpg)
![Western Blot: GABA-A R alpha 5 Antibody [NB300-195] - Mouse whole brain showing specific immunolabelling of the ~55kDa alpha 5-subunit of the GABAA R.](http://images.novusbio.com/fullsize/GABA-A-R-alpha-5-Antibody-Western-Blot-NB300-195-img0001.jpg "Western Blot: GABA-A R alpha 5 Antibody [NB300-195] - Mouse whole brain showing specific immunolabelling of the ~55kDa alpha 5-subunit of the GABAA R.")
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-Western-Blot-NBP2-37465-img0003.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of liver cancer tissues using ENO2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-Immunohistochemistry-NBP2-37465-img0006.jpg)
![Flow Cytometry: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-Flow-Cytometry-NBP2-37465-img0004.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa)](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-Western-Blot-NBP2-37465-img0002.jpg)
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of lung cancer tissues using ENO2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-Immunohistochemistry-NBP2-37465-img0005.jpg)
![ELISA: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-ELISA-NBP2-37465-img0001.jpg)
![Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS917] - Analysis of anti-GRM7 / MGLUR7 antibody with human adrenal at dilution 7 ug/ml.](http://images.novusbio.com/fullsize/mGluR7-Antibody-Immunohistochemistry-Paraffin-NLS917-img0005.jpg "Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS917] - Analysis of anti-GRM7 / MGLUR7 antibody with human adrenal at dilution 7 ug/ml.")
![Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS917] - Analysis of anti-GRM7 / MGLUR7 antibody with human liver at dilution 7 ug/ml.](http://images.novusbio.com/fullsize/mGluR7-Antibody-Immunohistochemistry-Paraffin-NLS917-img0004.jpg)
![Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-278202416285276.jpg "Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32207) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2982024142099.jpg)
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunocytochemistry analysis of HeLa cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2920241048483.jpg)
![Flow Cytometry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Flow cytometric analysis of HeLa cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32207, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-29202416372168.jpg)
![Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS921] - Anti-GRM7 / MGLUR7 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR7-Antibody-Immunohistochemistry-Paraffin-NLS921-img0005.jpg "Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS921] - Anti-GRM7 / MGLUR7 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS921] - Analysis of anti-mGluR7 antibody with human brain, neurons and glia.](http://images.novusbio.com/fullsize/mGluR7-Antibody-Immunohistochemistry-Paraffin-NLS921-img0001.jpg)
![Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS921] - Analysis of anti-MGLUR7 antibody with human adrenal.](http://images.novusbio.com/fullsize/mGluR7-Antibody-Immunohistochemistry-Paraffin-NLS921-img0003.jpg)
![Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS921] - Anti-GRM7 / MGLUR7 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR7-Antibody-Immunohistochemistry-Paraffin-NLS921-img0004.jpg)
![Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS921] - Brain, Hypothalamus](http://images.novusbio.com/fullsize/mGluR7-Antibody-Immunohistochemistry-Paraffin-NLS921-img0006.jpg)
![Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS921] - Eye, Retina](http://images.novusbio.com/fullsize/mGluR7-Antibody-Immunohistochemistry-Paraffin-NLS921-img0007.jpg)
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-168202375610..jpg "Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.")
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)- Human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-16820237586..jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3 treated with PDGFA 5 ug/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-168202375944.jpg)
![<b>Biological Strategies Validation. </b>Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved A431, untreated (-); treated with EGF (200 ng/mL, 15 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower).](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238120.jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 ug/mL, 5 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238442..jpg)
![Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3 treated with PDGFA (5 ug/mL, 5 min; +), using NBP3-21480 at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-136202422345072.png)
![Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5000 ng/mL, 5min; +) (line B); treated with PDGFA and -phosphatase (line C) using NBP3-21480 at 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-23520251628115.png)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-54559-img0002.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).")
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (4C4.9 + S100B/1012)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunohistochemistry-Paraffin-NBP2-54559-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-54559-img0001.jpg)
![Western Blot: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Azide-and-BSA-Free-Western-Blot-NBP2-54399-img0002.jpg "Western Blot: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).")
![Immunohistochemistry-Paraffin: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54399-img0001.jpg)