| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05210-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40775SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92715SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87753-25ul | |||
![Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Western-Blot-NBP2-67378-img0008.jpg "Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0007.jpg)
![Flow Cytometry: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of Jurkat cells with ERK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Flow-Cytometry-NBP2-67378-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded human breast tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0005.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded human colon cancer tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0006.jpg)
![Western Blot: NF-M Antibody (3H11) [NB300-134] - Neuronal tissue lysates using mouse mAb to NF-M, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.](http://images.novusbio.com/fullsize/NF-M-Antibody-3H11-Western-Blot-NB300-134-img0004.jpg "Western Blot: NF-M Antibody (3H11) [NB300-134] - Neuronal tissue lysates using mouse mAb to NF-M, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.")
![Immunohistochemistry Free-Floating: NF-M Antibody (3H11) [NB300-134] - Adult rat frontal cortex section stained with mouse mAb to neurofilament NF-M, dilution 1:5,000 in green, and costained with chicken pAb to neurofilament NF-H, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-134 antibody labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the NF-H antibody stains the network of neuronal axons only.](http://images.novusbio.com/fullsize/NF-M-Antibody-3H11-Immunohistochemistry-NB300-134-img0003.jpg)
![Western Blot: 160kDa Neurofilament Medium Antibody (3H11) [NB300-134] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including NB300-134 (lane 3).](http://images.novusbio.com/fullsize/160kDa-Neurofilament-Medium-Antibody-3H11-Western-Blot-NB300-134-img0002.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample(30ug whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0007.jpg "Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample(30ug whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody [NBP1-31465] - Analysis of methanol-fixed HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31465-img0003.jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody [NBP1-31465] - Human ovarian cancer, using NDUFS4 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunohistochemistry-Paraffin-NBP1-31465-img0004.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0005.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - 50 ug Rat heart lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0006.jpg)
![Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Western-Blot-NBP1-05210-img0006.jpg "Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody (9B12) [NBP1-05210] - Mixed neuron/glial cultures stained with NBP1-05210 (red) and rabbit GFAP antibody NB300-141 (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue).](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Immunocytochemistry-Immunofluorescence-NBP1-05210-img0003.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody (9B12) [NBP1-05210] - Analysis of a rat brain coronal section of the third ventricle stained with mouse monoclonal antibody to phosphorylated NF-H, dilution 1:5,000 (Green). Hoechst staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibody. The antibody is a robust marker of the axons of neuronal cells.](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Immunohistochemistry-Free-Floating-NBP1-05210-img0005.jpg)
![Wallerian degeneration and mitochondrial degeneration in axon for rat’s distal nerve stump. a, Representative images of toluidine blue staining, immunohistochemistry (NF-H), electron microscopy, before (pre) and 1, 2, and 14 d after axotomy. Immunohistochemistry was obtained the same results for three individuals. Scale bar, * 10 μm, ** 2 μm. Arrows, myelinated axon; arrowheads, demyelinated Schwann cell. b, Quantification of the myelinated axon. c, d, Quantification of the G-ratio [c: cumulative G-ratio per individual, d: scatter plot, Spearman’s rank correlation coefficient and p values showing G-ratio of individual myelinated axons against axon diameter (n = 400)]. The histologic WD was observed significantly from 2 d after axotomy. In axons, before (pre) and 1 and 2 d after axotomy were evaluated, except for after 14 d with completely collapsed axon morphology (n = 4 rat per group). e, Representative images of mitochondrial findings, before (pre) and 1, 2, and 14 d after axotomy. Evaluation was performed in axons and Schwann cells separately. Scale bar, * 200 nm. f, Violin plot of mitochondrial diameter (nm; n = 200). Cumulative mitochondria diameter per individual are also shown (n = 4 rat per group). g, Quantitative analysis of atypical mitochondria (%; n = 4 rat per group). In axons, mitochondria were significantly degenerated from 1 d after axotomy, whereas in Schwann cells, mitochondria were not significantly degenerated until 14 d after axotomy. All histologic evaluations were performed 3 mm distal to the sectional end and corresponding uninjured nerve. Error bars indicate SD; *p < 0.05, ***p < 0.001, one-way ANOVA followed by the Tukey’s post hoc test (for comparison of axons and Schwann cells, respectively). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36894321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05210_mouse-monoclonal-nf-h-antibody-9b12-immunocytochemistry-immunofluorescence-132202617595724.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (7711) diluted at 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (7711) diluted at 1:500.")
![<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [7711] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0001.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Glutamine Synthetase antibody [GT7711] detects Glutamine Synthetase protein in glial cells by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron & glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine Synthetase stained by Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-910202419593549.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:250000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-91020242063368.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Glutamine Synthetase antibody [GT7711] detects Glutamine Synthetase protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Glutamine Synthetase stained by Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-91020242041239.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-paraffin-26112025199277.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-frozen-261120251823624.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-western-blot-2611202518422920.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-paraffin-261120251844100.jpg)
![Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-immunohistochemistry-paraffin-261120251995415.jpg)
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40775] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/DISC1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40775-img0004.jpg "Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40775] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).")
![Immunohistochemistry: DISC1 Antibody [NB110-40775] - Staining of DISC1 in mouse brain neurons, glial cells, and white matter. 40X](http://images.novusbio.com/fullsize/DISC1-Antibody-Immunohistochemistry-NB110-40775-img0003.jpg)
![Western Blot: Secretagogin Antibody [NBP2-20286] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Secretagogin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Western-Blot-NBP2-20286-img0009.jpg "Western Blot: Secretagogin Antibody [NBP2-20286] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Secretagogin antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Secretagogin stained by Secretagogin antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20286-img0008.jpg)
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Secretagogin protein stained by Secretagogin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0010.jpg)
![Western Blot: Secretagogin Antibody [NBP2-20286] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Western-Blot-NBP2-20286-img0005.jpg)
![Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP2-20286] - Human colon carcinoma, using SCGN antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Paraffin-NBP2-20286-img0002.jpg)
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Secretagogin protein stained by Secretagogin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0006.jpg)
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Secretagogin stained by Secretagogin antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0007.jpg)
![Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Western blot shows lysates of 293T human embryonic kidney parental cell line and PARK7 knockout (KO) 293T cell line. PVDF membrane was probed with Mouse Anti-Human PARK7 Monoclonal Antibody (Catalog # NBP1-92715) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PARK7 at approximately 23 kDa (as indicated) in the parental 293T cell line, but is not detectable in the knockout 293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Knockout-Validated-NBP1-92715-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Western blot shows lysates of 293T human embryonic kidney parental cell line and PARK7 knockout (KO) 293T cell line. PVDF membrane was probed with Mouse Anti-Human PARK7 Monoclonal Antibody (Catalog # NBP1-92715) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PARK7 at approximately 23 kDa (as indicated) in the parental 293T cell line, but is not detectable in the knockout 293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Analysis of whole brain and cell lysates using mouse mAb against DJ1/Park7, NBP1-92715, dilution 1:5,000 in green. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The DJ1 antibody detects protein with apparent molecular weight of 21kDa but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken pAb to vimentin, dilution 1;5,000 in red, revealing a single band at about 50kDa present in all lanes, though at much lower levels in the tissue lysates.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Western-Blot-NBP1-92715-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Park7(DJ-1) Antibody (4H4) [NBP1-92715] - HeLa cells stained with NBP1-92715 (green), chicken antibody to Vimentin (NB300-223, red) and DNA (blue). NBP1-92715 reveals strong cytoplasmic staining for Park7(DJ-1).](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Immunocytochemistry-Immunofluorescence-NBP1-92715-img0003.jpg)
![Simple Western: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Lane view shows a specific band for Park7 (DJ-1) in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Simple-Western-NBP1-92715-img0004.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis. Sample: Paraffin-embedded dog tissues. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249383165.jpg "Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis. Sample: Paraffin-embedded dog tissues. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249351313.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-21202494086.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249362757.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis. Sample: Paraffin-embedded cat brain. SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249351325.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-212024943287.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249415919.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Non-transfected (-) and transfected (+) A549 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249425435.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-2120249403899.jpg)
![Western Blot: SERCA2 ATPase Antibody (HL2352) - Azide and BSA Free [NBP3-25678] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (NBP3-25678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25678_rabbit-serca2-atpase-mab-hl2352-azide-and-bsa-free-212024941591.jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025163536..jpg "<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-294202516917..jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025161213..jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2802) [NBP3-45161] - Tau antibody [HL2802] detects Tau protein at cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2802] diluted at 1:250.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2942025161556..jpg)
![Immunohistochemistry: Rabbit Tau mAb (HL2802) [NBP3-45161] - Tau antibody [HL2802] detects Tau protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Tau stained by Tau antibody [HL2802] diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunohistochemistry-paraffin-2942025161950..jpg)
![Western Blot: Rabbit Tau mAb (HL2802) [NBP3-45161] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2802] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-western-blot-2942025162224..jpg)
![Tau antibody [HL2802] detects Tau protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: Tau stained by Tau antibody [HL2802] (NBP3-45161) diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32618_rabbit-polyclonal-protein-phosphatase-1-beta-antibody-immunohistochemistry-frozen-141120251855420.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Analysis in human cerebral cortex and cervix, uterine tissues using NBP2-33774 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-33774_rabbit-polyclonal-gfap-antibody-immunohistochemistry-paraffin-166202374820.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Analysis in human cerebral cortex and cervix, uterine tissues using NBP2-33774 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GFAP Antibody [NBP2-33774] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NBP2-33774-img0043.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NBP2-33774] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33774-img0017.jpg)
![Western Blot: GFAP Antibody [NBP2-33774] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NBP2-33774-img0016.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] - Staining of human hippocampus shows positivity in astrocytes.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-33774-img0039.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] - Staining of human pancreas shows no positivity as expected.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-33774-img0040.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Staining of human cervix, uterine shows no cytoplasmic positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-33774_rabbit-polyclonal-gfap-antibody-immunohistochemistry-paraffin-166202375644..jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Analysis in human cerebral cortex and endometrium tissues using NBP1-87753 antibody. Corresponding NEFM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Analysis in human cerebral cortex and endometrium tissues using NBP1-87753 antibody. Corresponding NEFM RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cell.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-NBP1-87753-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NF-M Antibody [NBP1-87753] - Analysis NBP1-87753 (A) shows similar pattern to independent antibody NBP1-87754 (B).](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0013.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0018.jpg)
![Western Blot: NF-M Antibody [NBP1-87753] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: NF-M Antibody [NBP1-87753] - Analysis in human cell line HEK 293 and human cell line A-431.](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0012.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebral cortex shows strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0015.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human prostate shows strong positivity in peripheral nerve.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0017.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0019.jpg)
