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![Western Blot: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.](http://images.novusbio.com/fullsize/NPM1-Antibody-7H10B9-Western-Blot-NBP1-47354-img0014.jpg "Western Blot: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.](http://images.novusbio.com/fullsize/NPM1-Antibody-7H10B9-Immunocytochemistry-Immunofluorescence-NBP1-47354-img0011.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis of human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NPM1-Antibody-7H10B9-Immunohistochemistry-Paraffin-NBP1-47354-img0012.jpg)
![Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Western-Blot-NBP2-34331-img0003.jpg "Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)")
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Analysis using the Azide and BSA Free version of NBP2-34331. Staining of human Tonsil stained with Cdk1 MAb (POH-1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Immunohistochemistry-Paraffin-NBP2-34331-img0001.jpg)
![Western Blot: UbcH10/UBE2C Antibody [NB100-1084] - (2ug/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Western-Blot-NB100-1084-img0004.jpg "Western Blot: UbcH10/UBE2C Antibody [NB100-1084] - (2ug/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic /Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1084-img0003.jpg)
![Flow Cytometry: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Flow-Cytometry-NB100-1084-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1084-img0002.jpg)
![Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NDC80-Antibody-Western-Blot-NB100-1100-img0003.jpg "Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunohistochemistry-Paraffin-NB100-1100-img0002.jpg)
![Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunohistochemistry-Paraffin-NB100-1100-img0001.jpg)
![Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in mouse testis.](http://images.novusbio.com/fullsize/Bub1-Antibody-Western-Blot-H00000699-D01P-img0007.jpg "Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in mouse testis.")
![Immunocytochemistry/Immunofluorescence: Bub1 Antibody [H00000699-D01P] - Analysis of antibody to BUB1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Bub1-Antibody-Immunocytochemistry-Immunofluorescence-H00000699-D01P-img0005.jpg)
![Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in Jurkat.](http://images.novusbio.com/fullsize/Bub1-Antibody-Western-Blot-H00000699-D01P-img0006.jpg)
![Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in transfected 293T cell line by BUB1 polyclonal antibody.Lane 1: BUB1 transfected lysate(122.40 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Bub1-Antibody-Western-Blot-H00000699-D01P-img0008.jpg)
![Proximity Ligation Assay: Bub1 Antibody [H00000699-D01P] - Analysis of protein-protein interactions between BUB1 and APC. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Bub1-Antibody-Proximity-Ligation-Assay-H00000699-D01P-img0004.jpg)
![Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) - Azide and BSA Free [NBP2-34711] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Azide-and-BSA-Free-Western-Blot-NBP2-34711-img0002.jpg "Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) - Azide and BSA Free [NBP2-34711] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)")
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) - Azide and BSA Free [NBP2-34711] - Formalin-paraffin human Tonsil stained with Cdk1 MAb (POH-1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34711-img0001.jpg)
![Western Blot: Ki67/MKI67 Antibody [NBP3-05538] - Western blot analysis of equal amounts of cell lysates probed with Ki67/MKI67 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67/MKI67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67/MKI67 is a rather unstable snd sjhort half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Western-Blot-NBP3-05538-img0002.jpg "Western Blot: Ki67/MKI67 Antibody [NBP3-05538] - Western blot analysis of equal amounts of cell lysates probed with Ki67/MKI67 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67/MKI67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67/MKI67 is a rather unstable snd sjhort half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.")
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NBP3-05538] - Immunofluorescent analysis of HeLa cells stained with Ki67/MKI67 Antibody at a dilution of 1:2,000 in red, and mouse mAb to beta-tubulin at a dilution of 1:5,000 in green. The blue is DAPI staining of nuclear DNA. The Ki67/MKI67 antibody detects Ki67/MKI67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67/MKI67 forms a coat around condensed chromosomes. Ki67/MKI67 is not detected in cells in the quiescent G0 state. The beta-tubulin antibody produces strong staining of cytoplasmic microtubules.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05538-img0001.jpg)
![Western Blot: NCAPH Antibody [NBP1-32573] - Sample(30 ug whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Western-Blot-NBP1-32573-img0005.jpg "Western Blot: NCAPH Antibody [NBP1-32573] - Sample(30 ug whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-32573] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32573-img0003.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-32573] - Human gastric cancer, using BRRN1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32573-img0004.jpg)
![Western Blot: PTTG1 Antibody [NBP2-20287] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PTTG1-Antibody-Western-Blot-NBP2-20287-img0001.jpg "Western Blot: PTTG1 Antibody [NBP2-20287] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PTTG1 Antibody [NBP2-20287] - Securin antibody detects PTTG1 protein at on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. Securin antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PTTG1-Antibody-Immunohistochemistry-Paraffin-NBP2-20287-img0004.jpg)
![Immunoprecipitation: PTTG1 Antibody [NBP2-20287] - Securin antibody immunoprecipitates Securin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of Securin protein by 2 ug of Securin antibody 12% SDS-PAGE The immunoprecipitated Securin protein was detected by Securin antibody diluted at 1:1000](http://images.novusbio.com/fullsize/PTTG1-Antibody-Immunoprecipitation-NBP2-20287-img0003.jpg)
![Immunoprecipitation: SA1 Antibody (1515) [NBP2-43751] - Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 ug of SA1 antibody [1515]. Western blot analysis was performed using SA1 antibody [1515] EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/SA1-Antibody-1515-Immunoprecipitation-NBP2-43751-img0004.jpg "Immunoprecipitation: SA1 Antibody (1515) [NBP2-43751] - Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 ug of SA1 antibody [1515]. Western blot analysis was performed using SA1 antibody [1515] EasyBlot anti-Mouse IgG was used as a secondary reagent.")
![Western Blot: SA1 Antibody (1515) [NBP2-43751] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-91020242011502.jpg)
![Western Blot: SA1 Antibody (1515) [NBP2-43751] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-91020242014628.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-western-blot-2611202518222011.jpg)
![SA1 antibody [GT1515] detects SA1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SA1 protein stained by SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-immunocytochemistry-immunofluorescence-2611202518272316.jpg)
![Immunocytochemistry/Immunofluorescence: PTTG1/2/3 Antibody [NBP2-69985] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining.](http://images.novusbio.com/fullsize/PTTG1-2-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69985-img0005.jpg "Immunocytochemistry/Immunofluorescence: PTTG1/2/3 Antibody [NBP2-69985] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining.")
![Immunohistochemistry-Paraffin: PTTG1/2/3 Antibody [NBP2-69985] - (2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PTTG1-2-3-Antibody-Immunohistochemistry-Paraffin-NBP2-69985-img0003.jpg)
![Flow Cytometry: PTTG1/2/3 Antibody [NBP2-69985] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PTTG1-2-3-Antibody-Flow-Cytometry-NBP2-69985-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PTTG1/2/3 Antibody [NBP2-69985] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining.](http://images.novusbio.com/fullsize/PTTG1-2-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69985-img0004.jpg)
![Immunohistochemistry-Paraffin: PTTG1/2/3 Antibody [NBP2-69985] - (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PTTG1-2-3-Antibody-Immunohistochemistry-Paraffin-NBP2-69985-img0002.jpg)
![Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Western-Blot-NBP2-46199-img0006.jpg "Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.")
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0005.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0001.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0002.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0003.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0004.jpg)