| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94633-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92868-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03458-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59339-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99308-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06040-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48104 | |||
![Immunocytochemistry/ Immunofluorescence: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Immunofluorescence analysis of HepG2 cells using Ubiquitin C Rabbit pAb (A3207) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94633_rabbit-polyclonal-ubiquitin-c-antibody-5220251930293.jpg "Immunocytochemistry/ Immunofluorescence: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Immunofluorescence analysis of HepG2 cells using Ubiquitin C Rabbit pAb (A3207) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Immunohistochemistry analysis of Ubiquitin C in paraffin-embedded rat large intestine tissue using Ubiquitin C Rabbit pAb (A3207) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-94633_rabbit-polyclonal-ubiquitin-c-antibody-52202519294580.jpg)
![Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Immunohistochemistry analysis of Ubiquitin C in paraffin-embedded human spleen tissue using Ubiquitin C Rabbit pAb (A3207) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-94633_rabbit-polyclonal-ubiquitin-c-antibody-52202519302949.jpg)
![Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Immunohistochemistry analysis of Ubiquitin C in paraffin-embedded mouse large intestine tissue using Ubiquitin C Rabbit pAb (A3207) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-94633_rabbit-polyclonal-ubiquitin-c-antibody-5220251931346.jpg)
![Western Blot: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Western blot analysis of various lysates using Ubiquitin C Rabbit pAb (A3207) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit (RM00020). Exposuretime: 45s.](http://images.novusbio.com/fullsize/nbp2-94633_rabbit-polyclonal-ubiquitin-c-antibody-5220251931389.jpg)
![Western Blot: COPG2 Antibody [NBP2-92868] - Analysis of extracts of various cell lines, using COPG2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/COPG2-Antibody-Western-Blot-NBP2-92868-img0005.jpg "Western Blot: COPG2 Antibody [NBP2-92868] - Analysis of extracts of various cell lines, using COPG2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: COPG2 Antibody [NBP2-92868] - Analysis of U-2 OS cells using COPG2 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/COPG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92868-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: COPG2 Antibody - Azide and BSA Free [NBP2-92868] - Immunofluorescence analysis of C6 cells using COPG2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92868_rabbit-polyclonal-copg2-antibody-42202514264485.jpg)
![Immunohistochemistry-Paraffin: COPG2 Antibody [NBP2-92868] - Rat brain using COPG2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/COPG2-Antibody-Immunohistochemistry-Paraffin-NBP2-92868-img0004.jpg)
![Immunohistochemistry-Paraffin: COPG2 Antibody [NBP2-92868] - Mouse brain using COPG2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/COPG2-Antibody-Immunohistochemistry-Paraffin-NBP2-92868-img0003.jpg)
![Western Blot: ERP29 Antibody (1J0D3) [NBP3-15906] - Western blot analysis of extracts of various cell lines, using ERP29 Rabbit mAb (NBP3-15906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ERP29-Antibody-1J0D3-Western-Blot-NBP3-15906-img0004.jpg "Western Blot: ERP29 Antibody (1J0D3) [NBP3-15906] - Western blot analysis of extracts of various cell lines, using ERP29 Rabbit mAb (NBP3-15906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] - Immunohistochemistry analysis of ERP29 in paraffin-embedded mouse brain tissue using ERP29 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15906_rabbit-erp29-mab-1j0d3-7220251245352.jpg)
![Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] - Immunohistochemistry analysis of ERP29 in paraffin-embedded human colon carcinoma tissue using ERP29 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15906_rabbit-erp29-mab-1j0d3-72202512435314.jpg)
![Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] - Immunohistochemistry analysis of ERP29 in paraffin-embedded human liver tissue using ERP29 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15906_rabbit-erp29-mab-1j0d3-72202512443339.jpg)
![Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] - Immunohistochemistry analysis of ERP29 in paraffin-embedded rat kidney tissue using ERP29 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15906_rabbit-erp29-mab-1j0d3-72202512443369.jpg)
![Immunohistochemistry: ERP29 Antibody (1J0D3) [NBP3-15906] - Immunohistochemistry analysis of ERP29 in paraffin-embedded human breast cancer tissue using ERP29 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15906_rabbit-erp29-mab-1j0d3-72202512432042.jpg)
![Western Blot: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Western blot analysis of lysates from 293T cells using [KO Validated] HSP40/DNAJB1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-03458_rabbit-polyclonal-hsp40-dnajb1-antibody-5220251628060.jpg "Western Blot: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Western blot analysis of lysates from 293T cells using [KO Validated] HSP40/DNAJB1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP40/DNAJB1 Antibody [NBP3-03458] - Analysis of extracts from normal (control) and DNAJB1 knockout (KO) 293T cells, using HSP40/DNAJB1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/HSP40-DNAJB1-Antibody-Knockout-Validated-NBP3-03458-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Immunofluorescence analysis of U2OS cells using [KO Validated] HSP40/DNAJB1 Rabbit pAb . Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03458_rabbit-polyclonal-hsp40-dnajb1-antibody-5220251638035.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Immunofluorescence analysis of C6 cells using [KO Validated] HSP40/DNAJB1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03458_rabbit-polyclonal-hsp40-dnajb1-antibody-5220251628034.jpg)
![Western Blot: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Western blot analysis of various lysates using [KO Validated] HSP40/DNAJB1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-03458_rabbit-polyclonal-hsp40-dnajb1-antibody-5220251638081.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody - BSA Free [HSP40/DNAJB1] - Immunofluorescence analysis of HeLa cells using [KO Validated] HSP40/DNAJB1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03458_rabbit-polyclonal-hsp40-dnajb1-antibody-52202516293954.jpg)
![Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Western-Blot-NBP2-59339-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Immunocytochemistry-Immunofluorescence-NBP2-59339-img0006.jpg)
![Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - A cell surface stain was performed on HeLa cells with Hsp70 / Hsc70 Antibody (1H11) NBP2-59339PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cell-Surface-NBP2-59339-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Hsp70 / Hsc70 Antibody (1H11) [NBP2-59339] - Clone 1H11: FITC conjugate. Tissue: Jurkat E6.1. Species: Human. Unfixed/non-permeablized cells were incubated with (A) Anti-HSP70 control antibody (not cell-surface-specific), or (B) Anti-HSP70 antibody, clone 1H11 for 40 min at 4 degrees C. Fixed/permeabilized cells were incubated with (C) Anti-HSP70 control antibody, or (D) Anti-HSP70 antibody, clone 1H11 or 40 min at 4 degrees C. Analysis shows that non-permeabilized cells show cell surface binding with 1H11 clone (NBP2-59339) but not with the control antibody, and the permeabilized cells show strong intracellular binding with the control antibody but much less with 1H11 clone (NBP2-59339). Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cytometry-NBP2-59339-img0002.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA8.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Azide-and-BSA-Free-Western-Blot-NBP2-70962-img0002.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA8.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA8 .](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70962-img0003.jpg)
![Immunohistochemistry: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70962-img0004.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Azide-and-BSA-Free-Western-Blot-NBP2-70962-img0001.jpg)
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Anti-Clathrin Heavy Chain 1/CHC17 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 191 kDa](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Western-Blot-NBP2-99308-img0007.jpg "Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Anti-Clathrin Heavy Chain 1/CHC17 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 191 kDa")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in rat kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99308-img0006.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in human adrenal gland with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99308-img0002.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in human colon with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99308-img0003.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99308-img0004.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in mouse kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99308-img0005.jpg)
![Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-288202412493458.jpg "Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32961) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-298202414161869.jpg)
![Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202410484855.jpg)
![Flow Cytometry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Flow cytometric analysis of SH-SY5Y cells labeling SNAP25. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32961, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202416372172.jpg)
![Western Blot: APBA3/Mint-3 Antibody [NBP3-06040] - Anti-APBA3 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:61 kDa](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Western-Blot-NBP3-06040-img0001.jpg "Western Blot: APBA3/Mint-3 Antibody [NBP3-06040] - Anti-APBA3 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:61 kDa")
![Immunohistochemistry-Paraffin: APBA3/Mint-3 Antibody [NBP3-06040] - Staining of human APBA3 in human adrenal gland with rabbit polyclonal antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Immunohistochemistry-Paraffin-NBP3-06040-img0004.jpg)
![Immunohistochemistry-Paraffin: APBA3/Mint-3 Antibody [NBP3-06040] - Staining of human APBA3 in human kidney with rabbit polyclonal antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Immunohistochemistry-Paraffin-NBP3-06040-img0003.jpg)
![Immunoprecipitation: APBA3/Mint-3 Antibody [NBP3-06040] - APBA3 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-APBA3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-APBA3 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa Observed band size :72 kDa](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Immunoprecipitation-NBP3-06040-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: APBA3/Mint-3 Antibody [NBP3-06040] - Staining of APBA3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06040 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06040_rabbit-polyclonal-apba3-mint-3-antibody-235202514492346.png)
![Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (NX2.1/690) - Azide and BSA Free [NBP2-47772] - Rat Lung stained with TTF-1 Monoclonal Antibody (NX2.1/690)](http://images.novusbio.com/fullsize/TTF-1-NKX2-1-Antibody-NX2-1-690-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47772-img0003.jpg "Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (NX2.1/690) - Azide and BSA Free [NBP2-47772] - Rat Lung stained with TTF-1 Monoclonal Antibody (NX2.1/690)")
![Immunohistochemistry-Paraffin: p23/PTGES3 Antibody [NBP2-19998] - Mouse testis. p23 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-Immunohistochemistry-Paraffin-NBP2-19998-img0007.jpg "Immunohistochemistry-Paraffin: p23/PTGES3 Antibody [NBP2-19998] - Mouse testis. p23 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: p23/PTGES3 Antibody [NBP2-19998] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-Western-Blot-NBP2-19998-img0005.jpg)
![Western Blot: p23/PTGES3 Antibody [NBP2-19998] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:5000](http://images.novusbio.com/fullsize/p23-PTGES3-Antibody-Western-Blot-NBP2-19998-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and p23 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p23 antibody [N1C3] (NBP2-19998) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19998_rabbit-polyclonal-p23-ptges3-antibody-western-blot-26112025196212.jpg)
![Western Blot: Hsp10 Antibody [NBP2-16890] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsp10-Antibody-Western-Blot-NBP2-16890-img0001.jpg "Western Blot: Hsp10 Antibody [NBP2-16890] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: HSP10/EPF Antibody [NBP2-16890] - Lung carcinoma. Cpn10 antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HSP10-EPF-Antibody-Immunohistochemistry-Paraffin-NBP2-16890-img0003.jpg)
![Cpn10 antibody detects Cpn10 protein at cytoplasm by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cpn10 protein stained by Cpn10 antibody (NBP2-16890) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16890_rabbit-polyclonal-hsp10-epf-antibody-immunocytochemistry-immunofluorescence-261120251843193.jpg)
![Cpn10 antibody detects Cpn10 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cpn10 protein stained by Cpn10 antibody (NBP2-16890) diluted at 1:100.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16890_rabbit-polyclonal-hsp10-epf-antibody-immunocytochemistry-immunofluorescence-261120251848487.jpg)
