| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87790-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08901 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22209-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24560SS | |||
![Western Blot: MEK1 Antibody [NBP1-87790] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/MEK1-Antibody-Western-Blot-NBP1-87790-img0009.jpg "Western Blot: MEK1 Antibody [NBP1-87790] - Analysis in human cell line U-87 MG.")
![Immunocytochemistry/Immunofluorescence: MEK1 Antibody [NBP1-87790] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/MEK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87790-img0007.jpg)
![Immunohistochemistry-Paraffin: MEK1 Antibody [NBP1-87790] - Staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/MEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-87790-img0001.jpg)
![Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemical staining of human small intestine shows no cytoplasmic positivity as expected.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunohistochemistry-NBP1-81264-img0006.jpg "Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemical staining of human small intestine shows no cytoplasmic positivity as expected.")
![Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemistry analysis in human cerebral cortex and small intestine tissues using NBP1-81264 antibody. Corresponding MAP2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunohistochemistry-NBP1-81264-img0002.jpg)
![Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunohistochemistry-NBP1-81264-img0003.jpg)
![Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity..](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunohistochemistry-NBP1-81264-img0004.jpg)
![Immunohistochemistry: MAP2 Antibody [NBP1-81264] - Immunohistochemical staining of human pancreas shows moderate to strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunohistochemistry-NBP1-81264-img0005.jpg)
![Immunofluorescence: MAP2 Antibody [NBP1-81264] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunofluorescence-NBP1-81264-img0001.jpg)
![Western Blot: CD45RA Antibody (K4B5) [NBP3-07159] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Mouse Monoclonal Antibody (K4B5)](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Western-Blot-NBP3-07159-img0004.jpg "Western Blot: CD45RA Antibody (K4B5) [NBP3-07159] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Mouse Monoclonal Antibody (K4B5)")
![Immunohistochemistry-Paraffin: CD45RA Antibody (K4B5) [NBP3-07159] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Mouse Monoclonal Antibody (K4B5).](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Immunohistochemistry-Paraffin-NBP3-07159-img0003.jpg)
![Flow Cytometry: CD45RA Antibody (K4B5) [NBP3-07159] - Flow Cytometric Analysis of Jurkat cells using CD45RA Mouse Monoclonal Antibody (K4B5) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Flow-Cytometry-NBP3-07159-img0001.jpg)
![Immunohistochemistry-Paraffin: CD45RA Antibody (K4B5) [NBP3-07159] - Formalin-fixed, paraffin-embedded human Spleen stained with CD45RA Mouse Monoclonal Antibody (K4B5).](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Immunohistochemistry-Paraffin-NBP3-07159-img0002.jpg)
![Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using JNK1 mouse mAb against A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate.](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-Western-Blot-NBP2-22209-img0004.jpg "Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using JNK1 mouse mAb against A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate.")
![Flow Cytometry: JNK1 Antibody (1E5) [NBP2-22209] - Flow cytometric analysis of HeLa cells using JNK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-Flow-Cytometry-NBP2-22209-img0001.jpg)
![Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using MAPK8 mAb against human JNK1 (AA: 227-380) recombinant protein. (Expected MW is 43.4 kDa)](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-Western-Blot-NBP2-22209-img0003.jpg)
![ELISA: JNK1 Antibody (1E5) [NBP2-22209] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-ELISA-NBP2-22209-img0002.jpg)
![Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/ERK1-[p-Thr202--p-Tyr204]-Antibody-Western-Blot-NB500-141-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.](http://images.novusbio.com/fullsize/ERK1-[p-Thr202--p-Tyr204]-Antibody-Immunohistochemistry-NB500-141-img0004.jpg)
![Western Blot: MEK1 Antibody [NBP2-24542] - Analysis of MEK1/MEK2 phosphorylation in A375 whole cell lysate. Lane A shows phospho MEK1/MEK2 (upper band), and the weakly detected nonphosphorylated form (lower band), using MEK1 phospho antibody at 3 ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 using this antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-Western-Blot-NBP2-24542-img0001.jpg "Western Blot: MEK1 Antibody [NBP2-24542] - Analysis of MEK1/MEK2 phosphorylation in A375 whole cell lysate. Lane A shows phospho MEK1/MEK2 (upper band), and the weakly detected nonphosphorylated form (lower band), using MEK1 phospho antibody at 3 ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 using this antibody.")
![Western Blot: MEK1 Antibody [NBP2-24560] - Analysis of MEK1/2 phosphorylation in A375 whole cell lysate. Lane A shows phosphorylated MEK1/2 (upper band), and the weakly detected nonphosphorylated form (lower band), using NBP2-24560 at 3 ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 using this antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-Western-Blot-NBP2-24560-img0001.jpg "Western Blot: MEK1 Antibody [NBP2-24560] - Analysis of MEK1/2 phosphorylation in A375 whole cell lysate. Lane A shows phosphorylated MEK1/2 (upper band), and the weakly detected nonphosphorylated form (lower band), using NBP2-24560 at 3 ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 using this antibody.")
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249432880.jpg "Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249403813.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Untransfected (-) and transfected (+) HeLa whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249415916.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - SEK1 / MKK4 antibody [HL1888] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-212024941452.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249355531.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249422682.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-89647_rabbit-polyclonal-rsk1-antibody-13520251661876.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249355552.jpg "Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-212024940882.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-212024938311.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat urinary bladder. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249413375.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249362735.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249403888.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0002.jpg)
![ICC/IF: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp3-13144_rabbit-tor-mtor-p-ser-2448-pab-immunocytochemistry-immunofluorescence-126202482926..jpg)
![Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Western-Blot-NBP3-16536-img0005.jpg "Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of U-2 OS cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunohistochemistry of paraffin-embedded human colon using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunohistochemistry-Paraffin-NBP3-16536-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of C6 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of NIH-3T3 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0003.jpg)