| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25986 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26000 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25988 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55503-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP3-44067AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-44067AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-44067AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-44067AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-44067AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44067 | |||
| BSA Free | NBP2-59782-25ul | |||
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) [NBP2-61502] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61502 at 0.5 ug/ml, showed a band of histone H3 trimethylated at Lysine 4 (K4me3) in HeLa cells. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys4-Antibody-RM137-Western-Blot-NBP2-61502-img0003.jpg "Western Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) [NBP2-61502] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61502 at 0.5 ug/ml, showed a band of histone H3 trimethylated at Lysine 4 (K4me3) in HeLa cells. Observed molecular weight is ~17 kDa.")
![Multiplex Immunoassay: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP2-61502] - RM137 specifically reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/nbp2-61502_rabbit-monoclonal-histone-h3-trimethyl-lys4-antibody-rm137-multiplex-immunoassay-18920252352114.png)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP2-61502] - A Peptide dotblot shows RM137 reacts only to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).](http://images.novusbio.com/fullsize/nbp2-61502_rabbit-monoclonal-histone-h3-trimethyl-lys4-antibody-rm137-dot-blot-18920252342352.png)
![Western Blot: Histone H3 [ac Lys4] Antibody (RM149) [NBP2-61542] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61542, showed a band of Histone H3 acetylated at Lysine 4 in treated HeLa. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys4-Antibody-RM149-Western-Blot-NBP2-61542-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys4] Antibody (RM149) [NBP2-61542] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61542, showed a band of Histone H3 acetylated at Lysine 4 in treated HeLa. Observed molecular weight is ~17 kDa.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys4] Antibody (RM149) - Azide and BSA Free [NBP2-61542] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM149 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61542_rabbit-monoclonal-histone-h3-ac-lys4-antibody-rm149-immunocytochemistry-immunofluorescence-18920252337401.png)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61530 at 2 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-RM155-Western-Blot-NBP2-61530-img0004.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61530 at 2 ug/ml. Observed molecular weight is ~16 kDa.")
![Multiplex Immunoassay: Histone H3 [Trimethyl Lys36] Antibody (RM155) - Azide and BSA Free [NBP2-61530] - RM155 specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/nbp2-61530_rabbit-monoclonal-histone-h3-trimethyl-lys36-antibody-rm155-multiplex-immunoassay-1892025233710.png)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (RM155) - Azide and BSA Free [NBP2-61530] - ChIP performed on HeLa cells using RM155, 5ug. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61530_rabbit-monoclonal-histone-h3-trimethyl-lys36-antibody-rm155-chromatin-immunoprecipitation-189202523444712.png)
![Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0003.jpg "Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Histone H3 (phospho Thr3) antibody detects Histone H3 (phospho Thr3) protein by western blot analysis. Un-treated (-) and treated (+, 0.1uM Calyculin for 30 minutes + phosphatase inhibitor) HeLa whole cell extracts (2 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0001.jpg)
![Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0002.jpg)
![SDS-Page: Recombinant Human Cyclophilin B Protein [NBC1-18424]](http://images.novusbio.com/fullsize/Recombinant-Human-Cyclophilin-B-Protein-SDS-Page-NBC1-18424-img0002.jpg "SDS-Page: Recombinant Human Cyclophilin B Protein [NBC1-18424]")
![Western Blot: HSP60 Antibody (S02-5B7) [NBP3-19656] - Western blot detection of HSP60 in Hela cell lysates using NBP3-19656 (1:1000 diluted). Predicted band size: 61kDa. Observed band size: 60kDa.](http://images.novusbio.com/fullsize/HSP60-Antibody-S02-5B7-Western-Blot-NBP3-19656-img0002.jpg "Western Blot: HSP60 Antibody (S02-5B7) [NBP3-19656] - Western blot detection of HSP60 in Hela cell lysates using NBP3-19656 (1:1000 diluted). Predicted band size: 61kDa. Observed band size: 60kDa.")
![Immunohistochemistry-Paraffin: HSP60 Antibody (S02-5B7) [NBP3-19656] - Immunohistochemistry of HSP60 in paraffin-embedded Human lung cancer tissue using NBP3-19656 at dilution 1/50](http://images.novusbio.com/fullsize/HSP60-Antibody-S02-5B7-Immunohistochemistry-Paraffin-NBP3-19656-img0001.jpg)
![Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of Histone H3 unmodified at Lys4.](http://images.novusbio.com/fullsize/nbp3-26014_rabbit-histone-h3-mab-rm186-azide-and-bsa-free-226202422174413.png "Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of Histone H3 unmodified at Lys4.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Unmodified Lys4] Antibody (RM186) - Azide and BSA Free [NBP3-26014] - Immunocytochemical staining of HeLa cells using Clone RM186 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-26014_rabbit-histone-h3-mab-rm186-azide-and-bsa-free-22620242311013.png)
![Immunohistochemistry-Paraffin: Transferrin Antibody (TF/3001) - Azide and BSA Free [NBP2-79912] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Transferrin Mouse Monoclonal Antibody (TF/3001).](http://images.novusbio.com/fullsize/Transferrin-Antibody-TF-3001-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79912-img0001.jpg "Immunohistochemistry-Paraffin: Transferrin Antibody (TF/3001) - Azide and BSA Free [NBP2-79912] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Transferrin Mouse Monoclonal Antibody (TF/3001).")
![Protein Array: Transferrin Antibody (TF/3001) - Azide and BSA Free [NBP2-79912] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAbto protein X is equal to 29.](http://images.novusbio.com/fullsize/Transferrin-Antibody-TF-3001-Azide-and-BSA-Free-Protein-Array-NBP2-79912-img0002.jpg)
![Western Blot: HSP60 Antibody [NBP2-55503] - Analysis using Anti-HSPD1 antibody NBP2-55503 (A) shows similar pattern to independent antibody NBP1-89730 (B).](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP2-55503-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HSP60 Antibody [NBP2-55503] - Analysis using Anti-HSPD1 antibody NBP2-55503 (A) shows similar pattern to independent antibody NBP1-89730 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP2-55503] - Staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55503-img0001.jpg)
![Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PPIB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-5479-Western-Blot-NBP2-59782-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PPIB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-5479-Western-Blot-NBP2-59782-img0001.jpg)