| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14062 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP3-49666AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-49666AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-49666AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-49666AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-49666AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15566-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25989 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25991 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26003 | |||
![Dot Blot: Histone H3 [Dimethyl Lys23] Antibody (RM171) - Azide and BSA Free [NBP3-26005] - A peptide dotblot shows RM171 reacts only to Histone H3 Dimethyl-Lysine 23 (K23me2). No cross reactivity with nonmodified Lysine 23 (K23Ctrl), monomethylated Lysine 23 (K23me1), or dimethylated Lysine 4 (K4me2).](http://images.novusbio.com/fullsize/nbp3-26005_rabbit-histone-h3-mab-rm171-azide-and-bsa-free-24620241332440.png "Dot Blot: Histone H3 [Dimethyl Lys23] Antibody (RM171) - Azide and BSA Free [NBP3-26005] - A peptide dotblot shows RM171 reacts only to Histone H3 Dimethyl-Lysine 23 (K23me2). No cross reactivity with nonmodified Lysine 23 (K23Ctrl), monomethylated Lysine 23 (K23me1), or dimethylated Lysine 4 (K4me2).")
![Western Blot: Histone H3 [Dimethyl Lys23] Antibody (RM171) - Azide and BSA Free [NBP3-26005] - Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using RM171 at 0.5 ug/mL, showed a band of Histone H3 dimethylated at Lysine 23 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26005_rabbit-histone-h3-mab-rm171-azide-and-bsa-free-22620242311019.png)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys23] Antibody (RM171) - Azide and BSA Free [NBP3-26005] - RM171 specifically reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/nbp3-26005_rabbit-histone-h3-mab-rm171-azide-and-bsa-free-2462024132645.png)
![Western Blot: GAPDH Antibody [NBP2-56153] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NBP2-56153-img0002.jpg "Western Blot: GAPDH Antibody [NBP2-56153] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NBP2-56153] - Staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56153-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: GAPDH Antibody [NBP2-56153] - Analysis using Anti-GAPDH antibody NBP2-56153 (A) shows similar pattern to independent antibody NBP2-48721 (B). Theoretical molecular weight: 36 kDa](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NBP2-56153-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC1 Antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/HDAC1-Antibody-PCRP-HDAC1-1B7-Immunocytochemistry-Immunofluorescence-NBP3-13936-img0002.jpg "Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC1 Antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).")
![Flow Cytometry: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Flow cytometric analysis of PFA-fixed HeLa cells. HDAC1 antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/HDAC1-Antibody-PCRP-HDAC1-1B7-Flow-Cytometry-NBP3-13936-img0001.jpg)
![Protein Array: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC1 Antibody (PCRP-HDAC1-1B7).](http://images.novusbio.com/fullsize/HDAC1-Antibody-PCRP-HDAC1-1B7-Protein-Array-NBP3-13936-img0004.jpg)
![Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Western-Blot-NBP3-15566-img0002.jpg "Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Chromatin Immunoprecipitation: HDAC1 Antibody (2W4I5) [NBP3-15566] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using HDAC1 antibody (NBP3-15566) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Chromatin-Immunoprecipitation-NBP3-15566-img0003.jpg)
![Western Blot: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-Western-Blot-NBP2-59210-img0001.jpg "Western Blot: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - ChIP assays were performed using human HeLa cells, the antibody against H3K79me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a region 1 kb upstream of the promoter and the coding region of the active GAPDH gene, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NBP2-59210-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K79me1. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,500.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-ELISA-NBP2-59210-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-Immunofluorescence-NBP2-59302-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - ChIP assays were performed using human HeLa cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the EIF4A2 and GAPDH genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-Chromatin-Immunoprecipitation-NBP2-59302-img0002.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - To test the specificity an ELISA was performed using a serial dilution of the monoclonal antibody against H3K27ac. The wells were coated with peptides containing the unmodified H3K27 region as well as the acetylated H3K27 and the acetylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-ELISA-NBP2-59302-img0005.jpg)
![Western Blot: beta-Actin [p Tyr53] Antibody [NBP3-23323] - Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23323_rabbit-beta-actin-p-tyr53-pab-199202312403723.jpg "Western Blot: beta-Actin [p Tyr53] Antibody [NBP3-23323] - Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: beta-Actin [p Tyr53] Antibody [NBP3-23323] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).](http://images.novusbio.com/fullsize/nbp3-23323_rabbit-beta-actin-p-tyr53-pab-19920231911470.jpg)
![Western Blot: Histone H3 [ac Lys36] Antibody (RM154) [NBP2-61541] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using NBP2-61541, showed a band of histone H3 acetylated at Lysine 36 in treated HeLa. Observed molecular weight is ~17 kDa](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys36-Antibody-RM154-Western-Blot-NBP2-61541-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys36] Antibody (RM154) [NBP2-61541] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using NBP2-61541, showed a band of histone H3 acetylated at Lysine 36 in treated HeLa. Observed molecular weight is ~17 kDa")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys26] Antibody (RM154) - Azide and BSA Free [NBP2-61541] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM154 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61541_rabbit-monoclonal-histone-h3-ac-lys36-antibody-rm154-immunocytochemistry-immunofluorescence-189202523394320.png)
![Western Blot: Histone H3 [Monomethyl Lys79] Antibody (RM147) [NBP2-61531] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61531 at 1 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys79-Antibody-RM147-Western-Blot-NBP2-61531-img0006.jpg "Western Blot: Histone H3 [Monomethyl Lys79] Antibody (RM147) [NBP2-61531] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61531 at 1 ug/ml. Observed molecular weight is ~17 kDa.")
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys79] Antibody (RM147) - Azide and BSA Free [NBP2-61531] - RM147 specifically reacts to Histone H3 monomethylated at Lysine 79. No cross reactivity with dimethylated Lysine 79 or trimethylated Lysine 79, or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp2-61531_rabbit-monoclonal-histone-h3-monomethyl-lys79-antibody-rm147-multiplex-immunoassay-189202523512117.png)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys79] Antibody (RM147) [NBP2-61531] - ChIP performed on HeLa cells using H3K79me1 antibody (NBP2-61531). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61531_rabbit-monoclonal-histone-h3-monomethyl-lys79-antibody-rm147-chromatin-immunoprecipitation-189202523512123.png)
![Dot Blot: Histone H3 [Dimethyl Lys18] Antibody (RM168) [NBP2-61514] - A Peptide dot blot shows NBP2-61514 only reacts to Histone. H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated. Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys18-Antibody-RM168-Dot-Blot-NBP2-61514-img0006.jpg "Dot Blot: Histone H3 [Dimethyl Lys18] Antibody (RM168) [NBP2-61514] - A Peptide dot blot shows NBP2-61514 only reacts to Histone. H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated. Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2).")
![Western Blot: Histone H3 [Dimethyl Lys18] Antibody (RM168) [NBP2-61514] - Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61514 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys18-Antibody-RM168-Western-Blot-NBP2-61514-img0004.jpg)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys18] Antibody (RM168) - Azide and BSA Free [NBP2-61514] - RM168 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/nbp2-61514_rabbit-monoclonal-histone-h3-dimethyl-lys18-antibody-rm168-multiplex-immunoassay-18920252337119.png)