| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26014 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26032 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25996 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26015 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55503-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44067 | |||
| BSA Free | NBP2-59782-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79912 | |||
![Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) [NBP2-61543] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using NBP2-61543, showed a band of Histone H3 unmodified at Lys4. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Unmodified-Lys4-Antibody-RM186-Western-Blot-NBP2-61543-img0006.jpg "Western Blot: Histone H3 [Unmodified Lys4] Antibody (RM186) [NBP2-61543] - Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant Histone H3.1 protein, using NBP2-61543, showed a band of Histone H3 unmodified at Lys4. Observed molecular weight is ~17 kDa.")
![Multiplex Immunoassay: Histone H3 [Unmodified Lys4] Antibody (RM186) [NBP2-61543] - NBP2-61543 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in Histone H3.](http://images.novusbio.com/fullsize/nbp2-61543_rabbit-monoclonal-histone-h3-unmodified-lys4-antibody-rm186-multiplex-immunoassay-18920252352112.png)
![Western Blot: Histone H3 [Crotonyl Lys9] Antibody (RM339) [NBP2-77404] - Western Blot using NBP2-77404 against H3K9cr [Crotonyl-Histone H3 (Lys9)]. Anti-Histone H3 and anti-G6PDH were used as controls. A crotonylation inducing metabolite was used to increase the H3K9cr signal. Theoretical molecular weight is ~15 kDa](http://images.novusbio.com/fullsize/Histone-H3-Crotonyl-Lys9-Antibody-RM339-Western-Blot-NBP2-77404-img0001.jpg "Western Blot: Histone H3 [Crotonyl Lys9] Antibody (RM339) [NBP2-77404] - Western Blot using NBP2-77404 against H3K9cr [Crotonyl-Histone H3 (Lys9)]. Anti-Histone H3 and anti-G6PDH were used as controls. A crotonylation inducing metabolite was used to increase the H3K9cr signal. Theoretical molecular weight is ~15 kDa")
![Dot Blot: Histone H3 [Crotonyl Lys9] Antibody (RM339) [NBP2-77404] - A Peptide dot blot showing NBP2-77404 reacts specifically to Histone H3 crotonylated at Lysine 9 (H3K9-Crotonyl), and NBP2-77404's cross-reactivity with different peptides.](http://images.novusbio.com/fullsize/Histone-H3-Crotonyl-Lys9-Antibody-RM339-Dot-Blot-NBP2-77404-img0002.jpg)
![Western Blot: Histone H3 [ac Lys27] Antibody (RM172) [NBP2-61549] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61549 at 1 ug/ml, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa. Observed molecular weight is ~17 kDa](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-RM172-Western-Blot-NBP2-61549-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys27] Antibody (RM172) [NBP2-61549] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61549 at 1 ug/ml, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa. Observed molecular weight is ~17 kDa")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys27] Antibody (RM172) - Azide and BSA Free [NBP2-61549] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM172 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61549_rabbit-monoclonal-histone-h3-ac-lys27-antibody-rm172-immunocytochemistry-immunofluorescence-189202523381724.png)
![Western Blot: Histone H3 [ac Lys23] Antibody (RM169) [NBP2-61544] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant Histone H3.3 (3). using NBP2-61544. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys23-Antibody-RM169-Western-Blot-NBP2-61544-img0004.jpg "Western Blot: Histone H3 [ac Lys23] Antibody (RM169) [NBP2-61544] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant Histone H3.3 (3). using NBP2-61544. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys23] Antibody (RM169) [NBP2-61544] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using NBP2-61544 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys23-Antibody-RM169-Immunocytochemistry-Immunofluorescence-NBP2-61544-img0003.jpg)
![Western Blot: HSP60 Antibody [NBP2-55503] - Analysis using Anti-HSPD1 antibody NBP2-55503 (A) shows similar pattern to independent antibody NBP1-89730 (B).](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP2-55503-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HSP60 Antibody [NBP2-55503] - Analysis using Anti-HSPD1 antibody NBP2-55503 (A) shows similar pattern to independent antibody NBP1-89730 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP2-55503] - Staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55503-img0001.jpg)
![Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PPIB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-5479-Western-Blot-NBP2-59782-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PPIB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-5479-Western-Blot-NBP2-59782-img0001.jpg)
![Immunohistochemistry-Paraffin: Transferrin Antibody (TF/3001) [NBP2-79778] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Transferrin Antibody (TF/3001).](http://images.novusbio.com/fullsize/Transferrin-Antibody-TF-3001-Immunohistochemistry-Paraffin-NBP2-79778-img0001.jpg "Immunohistochemistry-Paraffin: Transferrin Antibody (TF/3001) [NBP2-79778] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Transferrin Antibody (TF/3001).")
![Protein Array: Transferrin Antibody (TF/3001) [NBP2-79778] - Analysis of Protein Array containing >19,000 full-length human proteins using Transferrin Antibody (TF/3001) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5](http://images.novusbio.com/fullsize/Transferrin-Antibody-TF-3001-Protein-Array-NBP2-79778-img0002.jpg)
![Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Hs-8-Flow-Cytometry-NB500-456-img0001.jpg "Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.")
![Western Blot: Histone H3 [ac Lys23] Antibody (JE46-39) [NBP3-32428] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500 ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 500 ng/mL TSA for 4 hours cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 15 kDa Observed band size: 15/14 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32428) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32428_rabbit-histone-h3-ac-lys23-mab-je46-39-278202415295541.jpg "Western Blot: Histone H3 [ac Lys23] Antibody (JE46-39) [NBP3-32428] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500 ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 500 ng/mL TSA for 4 hours cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 15 kDa Observed band size: 15/14 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32428) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys23] Antibody (JE46-39) [NBP3-32428] - Immunocytochemistry analysis of HeLa cells treated with or without 500 ng/mL TSA for 4 hours labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32428) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32428_rabbit-histone-h3-ac-lys23-mab-je46-39-2920241192342.jpg)