| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03711-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48966-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43961 | |||
| BSA Free | NBP2-59777-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43847 | |||
| BSA Free | NBP2-61620-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90058-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61620 | |||
| BSA Free | NBP2-61620-25ul | |||
![Western Blot: Lamin B1 Antibody [NBP3-03711] - Analysis of extracts of various cell lines, using Lamin B1 antibody at 1:1000 dilution.L929 cells were treated by staurosporine(1 uM) for 3 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Western-Blot-NBP3-03711-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: Lamin B1 Antibody [NBP3-03711] - Analysis of extracts of various cell lines, using Lamin B1 antibody at 1:1000 dilution.L929 cells were treated by staurosporine(1 uM) for 3 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time: 30s.")
![<b>Biological Strategies Validation. </b>Western Blot: Lamin B1 Antibody [NBP3-03711] - Analysis of extracts of various cell lines, using Lamin B1 antibody at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37c for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Western-Blot-NBP3-03711-img0009.jpg)
![Immunoprecipitation: Lamin B1 Antibody [NBP3-03711] - Analysis of 300ug extracts of MCF7 cells using 3ug Lamin B1 antibody. Western blot was performed from the immunoprecipitate using Lamin B1 antibody at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunoprecipitation-NBP3-03711-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Immunofluorescence analysis of U2OS cells using Lamin B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-5220251639644.jpg)
![Immunohistochemistry: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Lamin B1 Rabbit pAb at dilution of 1:150 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-5220251632145.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Confocal immunofluorescence analysis of HeLa cells using Lamin B1 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-522025164022.jpg)
![Immunoprecipitation: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Immunoprecipitation of Lamin B1 in 300 ug extracts from Jurkat cells using 0.5 ug Lamin B1 Rabbit pAb . Western blot analysis was performed using Lamin B1 Rabbit pAb at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-52202516321471.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Immunofluorescence analysis of C6 cells using Lamin B1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-5220251628012.jpg)
![Chromatin Immunoprecipitation: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Chromatin immunoprecipitation was performed with cross-linked chromatin from HeLa, using Lamin-B1 Rabbit mAb and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-52202516334162.jpg)
![Chromatin Immunoprecipitation: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Lamin B1 Rabbit pAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-52202516283214.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Confocal immunofluorescence analysis of HeLa cells using Lamin B1 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-5220251640283.jpg)
![Immunohistochemistry: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Lamin B1 Rabbit pAb at dilution of 1:150 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-522025164025.jpg)
![Immunohistochemistry: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Lamin B1 Rabbit pAb at dilution of 1:150 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-52202516283291.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Immunofluorescence analysis of PC-12 cells using Lamin B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-5220251639673.jpg)
![Immunoprecipitation: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug Lamin B1 antibody . Western blot was performed from the immunoprecipitate using Lamin B1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-5220251638080.jpg)
![Western Blot: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Western blot analysis of various lysates using Lamin B1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1min.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-5220251638058.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody - Azide and BSA Free [Lamin B1] - Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03711_rabbit-polyclonal-lamin-b1-antibody-52202516283299.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Analysis in human lymph node and skeletal muscle tissue. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-48966-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Analysis in human lymph node and skeletal muscle tissue. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lamin B1 Antibody [NBP2-48966] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Western-Blot-NBP2-48966-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody [NBP2-48966] - Staining of human cell line MCF7 shows localization to nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48966-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Staining of human skeletal muscle shows moderate to strong positivity in nuclear membrane in myocytes.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-48966-img0008.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Staining of human lymph node shows moderate to strong positivity in nuclear membrane in non-germinal center cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-48966-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Staining of human skin shows moderate to strong positivity in nuclear membrane in keratinocytes.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-48966-img0006.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP2-48966] - Staining of human small intestine shows moderate to strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-48966-img0007.jpg)
![Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Western-Blot-NBP2-59777-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human liver shows cytoplasmic immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0005.jpg)
![Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2 and mouse cell line NIH-3T3.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Western-Blot-NBP2-59777-img0006.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0001.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0002.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human stomach shows strong cytoplasmic immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0003.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human prostate shows cytoplasmic positivity in the epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0004.jpg)
![Western Blot: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Western blot analysis of extracts of various cell lines, using Lamin B1 Rabbit mAb (NBP3-15393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Western-Blot-NBP3-15393-img0004.jpg "Western Blot: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Western blot analysis of extracts of various cell lines, using Lamin B1 Rabbit mAb (NBP3-15393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded mouse testis using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Immunohistochemistry-Paraffin-NBP3-15393-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded rat spleen using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Immunohistochemistry-Paraffin-NBP3-15393-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded human colon using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Immunohistochemistry-Paraffin-NBP3-15393-img0002.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Rat liver using Lamin B1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517202977.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-522025171890.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using Lamin B1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517284935.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Human colon using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517202913.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517223953.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Lamin B1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517202933.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517152732.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using Lamin B1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517172857.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517273578.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Human brain using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517251911.jpg)
![Western Blot: COX4 Antibody (CL3515) [NBP2-61620] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Western-Blot-NBP2-61620-img0006.jpg "Western Blot: COX4 Antibody (CL3515) [NBP2-61620] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2")
![Immunocytochemistry/Immunofluorescence: COX4 Antibody (CL3515) [NBP2-61620] - Staining of MCF7 cells using the Anti-COX4I1 monoclonal antibody, showing specific staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunocytochemistry-Immunofluorescence-NBP2-61620-img0007.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human skin shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-Paraffin-NBP2-61620-img0005.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human prostate shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0001.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human placenta shows strong cytoplasmic immunoreactivity in trophoblasts.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0002.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0003.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0004.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0004.jpg "Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP2-54621] - HeLa cells were stained with the antibody against HDAC1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunofluorescence-NBP2-54621-img0006.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP2-54621] - ChIP was performed with the antibody against HDAC1 on sheared chromatin from 4,000,000 HeLa cells. An antibody titration consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54621-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (25 ug, lane 1) and nuclear extracts (25 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0003.jpg)
![ELISA: HDAC1 Antibody [NBP2-54621] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against HDAC1, crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:75,000.](http://images.novusbio.com/fullsize/HDAC1-Antibody-ELISA-NBP2-54621-img0002.jpg)
![Protein Array: HDAC1 Antibody [NBP2-54621] - The specificity of the antibody against HDAC1 was demonstrated using a human protein microarray containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. The figure shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 5 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Protein-Array-NBP2-54621-img0005.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0004.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human breast carcinoma with rabbit monoclonal antibody at 1:200).](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0001.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human liver with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human colon carcinoma with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0003.jpg)
![Western Blot: COX4 Antibody (9X9I0) [NBP3-15416] - Western blot analysis of extracts of various cell lines, using COX4 Rabbit mAb (NBP3-15416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/COX4-Antibody-9X9I0-Western-Blot-NBP3-15416-img0002.jpg "Western Blot: COX4 Antibody (9X9I0) [NBP3-15416] - Western blot analysis of extracts of various cell lines, using COX4 Rabbit mAb (NBP3-15416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: COX4 Antibody (9X9I0) [NBP3-15416] - Immunohistochemistry of paraffin-embedded human colon carcinoma using COX4 Rabbit mAb (NBP3-15416) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/COX4-Antibody-9X9I0-Immunohistochemistry-Paraffin-NBP3-15416-img0001.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-5220251726031.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-52202517223979.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human colon using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-52202517284940.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human kidney using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-52202517162399.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-5220251726025.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89504] - Staining of human colon, liver, lymph node and testis using Anti-PCNA antibody NBP1-89504 (A) shows similar protein distribution across tissues to independent antibody NBP1-89503 (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-89504-img0016.jpg "Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89504] - Staining of human colon, liver, lymph node and testis using Anti-PCNA antibody NBP1-89504 (A) shows similar protein distribution across tissues to independent antibody NBP1-89503 (B).")
![Western Blot: PCNA Antibody (BLR075G) [NBP2-78712] - Detection of human PCNA by WB of A-549, Jurkat, HEK293T, RKO, K-562, MCF-7, HeLa, U2OS, TCMK-1, and NIH 3T3 lysate using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).](http://images.novusbio.com/fullsize/PCNA-Antibody-BLR075G-NBP2-78712-img0004.jpg "Western Blot: PCNA Antibody (BLR075G) [NBP2-78712] - Detection of human PCNA by WB of A-549, Jurkat, HEK293T, RKO, K-562, MCF-7, HeLa, U2OS, TCMK-1, and NIH 3T3 lysate using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).")
![Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712] - Antibody: Rabbit anti-PCNA recombinant monoclonal antibody [BLR075G]Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain:DAPI.](http://images.novusbio.com/fullsize/PCNA-Antibody-BLR075G-Immunohistochemistry-Paraffin-NBP2-78712-img0005.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712] - Human ovarian carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/PCNA-Antibody-BLR075G-NBP2-78712-img0001.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712] - Human prostate carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/PCNA-Antibody-BLR075G-NBP2-78712-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712] - Hhuman OVCAR-3 cells by ICC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB](http://images.novusbio.com/fullsize/PCNA-Antibody-BLR075G-NBP2-78712-img0003.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2](http://images.novusbio.com/fullsize/nbp3-43847_mouse-cox4-mab-cl3515-azide-and-bsa-free-282202512104221.jpg "Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2")
![Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2682024158269.jpg "Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL")
![Immunohistochemistry: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-PCNA antibody (NBP3-32725) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32725) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2982024149267.jpg)
![Immunocytochemistry/ Immunofluorescence: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunocytochemistry analysis of HeLa cells labeling PCNA with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-29202410484872.jpg)
![Western Blot: PCNA Antibody (A6-G11-R) [NBP3-32725] - Western blot analysis of PCNA on different lysates with Mouse anti-PCNA antibody (NBP3-32725) at 1/20,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: HCT 116 cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: RAW264.7 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: L6 cell lysate (15 µg/Lane) Lane 8: Mouse spleen tissue lysate (15 µg/Lane) Lane 9: Rat spleen tissue lysate (15 µg/Lane) Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 11 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-3920248485013.jpg)